Determination of Size and Concentration of Gold Nanoparticles from UV−Vis Spectra

Haiss, Wolfgang; Thanh, Nguyễn Thị Kim; Aveyard, Jenny; Fernig, David G.

doi:10.1021/ac0702084

Cited by 3,223 publications

(2,732 citation statements)

References 22 publications

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“…Therefore, smaller AuNPs were prepared by sodium borohydride reduction. 15 A mixture of 6.75 ml of 0.075 wt % sodium borohydride (Sigma-Aldrich, USA) and 18.75 ml of 38.8 mM sodium citrate was added to 250 ml of 250 µM HAuCl 4 in an ice bath, and the reaction mixture was stirred for 10 min. The average particle diameter of the AuNPs prepared by the sodium borohydride reduction method was estimated to be approximately 7 nm (AuNP 7 ) based on the UV-vis spectrum of the AuNP solution.…”

Section: Preparation Of Aunp-modified Electrodesmentioning

confidence: 99%

The Effect of Particle Size on the Direct Electron Transfer Reactions of Metalloproteins Using Au Nanoparticle-Modified Electrodes

et al. 2012

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For cyt c, the reduction and oxidation currents for the AuNP 7 -modified electrode were small compared to the other AuNP sizes (AuNP 15 and AuNP 70 ) with the same roughness factor (R f ). For BOD and FDH, the AuNP 70 -modified electrode had the best performance in terms of the catalytic current at the same R f . The results demonstrate that the size of spaces on the electrode surface is a very important factor governing the electron transfer reactions of proteins on an AuNP-modified electrode.

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Section: Preparation Of Aunp-modified Electrodesmentioning

confidence: 99%

The Effect of Particle Size on the Direct Electron Transfer Reactions of Metalloproteins Using Au Nanoparticle-Modified Electrodes

et al. 2012

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“…The ability to detect trace amounts of target deoxyribonucleic acid (DNA) sequences in a highly selective manner is of evergrowing interest for rapid detection of disease [1][2][3][4][5][6][7][8][9][10][11]. Herein, we report a method for rapid detection of DNA with ultrahigh sensitivity and specificity based on a novel strategy involving a combination of a polymerase chain reaction (PCR) and Au nanoparticles (Au NPs).…”

Section: Introductionmentioning

confidence: 99%

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Cai

Zhang

et al. 2010

Nano Res.

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We report a novel method for rapid, colorimetric detection of a specific deoxyribonucleic acid (DNA) sequence by carrying out a polymerase chain reaction in the presence of gold nanoparticles functionalized with two primers. Extension of the primers when the target DNA is present as a template during the polymerase chain reaction process affords the complementary sequences on the gold nanoparticle surfaces and results in the formation of gold nanoparticle aggregates with a concomitant color change from red to pinkish/purple. This method provides a convenient and straightforward solution for ultrasensitive DNA detection without any further post-treatment of the polymerase chain reaction products being necessary, and is a promising tool for rapid disease diagnostics and gene sequencing.

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“…Then, the AuNPs aqueous solution has been concentrated by centrifugation for 10 min (7,800 rpm-10,000g). After this centrifugation step, a relatively high concentration of AuNPs has been estimated about 0.96±0.12 nmol/L from optical absorption spectra [23].…”

Section: Methodsmentioning

confidence: 99%

Nanostructured and nanopatterned gold surfaces: application to the surface-enhanced Raman spectroscopy

et al. 2013

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Surface-enhanced Raman spectroscopy (SERS) has enormous potential for a range of applications where high sensitivity needs to be combined with good discrimination between molecular targets. However, the SERS technique has trouble finding its industrial development, as was the case with the surface plasmon resonance technology. The main reason is the difficulty to produce stable, reproducible, and highly efficient substrates for quantitative measurements. In this paper, we report a method to obtain two-dimensional regular nanopatterns of gold nanoparticles (AuNPs). The resulting patterns were evaluated by SERS. Our bottom-up strategy was divided into two steps: (a) nanopatterning of the substrate by e-beam lithography and (b) electrostatic adsorption of AuNPs on functionalized substrates. This approach enabled us to highlight the optimal conditions to obtain monolayer, rows, or ring of AuNPs, with homogeneous distribution and high density (800 AuNPs/μm 2 ). The nanostructure distributions on the substrates were displayed by scanning electron microscopy and atomic force microscopy images. Optical properties of our nanostructures were characterized by visible extinction spectra and by the measured enhancements of Raman scattering. Finally, we tried to demonstrate experimentally that, to observe a significant enhancement of SERS, the gold diffusers must be extremely closer. If electron beam lithography is a very attractive technique to perform reproducible SERS substrates, the realization of pattern needs a very high resolution, with distances between nanostructures probably of less than 20 nm.

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Determination of Size and Concentration of Gold Nanoparticles from UV−Vis Spectra

Cited by 3,223 publications

References 22 publications

The Effect of Particle Size on the Direct Electron Transfer Reactions of Metalloproteins Using Au Nanoparticle-Modified Electrodes

The Effect of Particle Size on the Direct Electron Transfer Reactions of Metalloproteins Using Au Nanoparticle-Modified Electrodes

One-pot polymerase chain reaction with gold nanoparticles for rapid and ultrasensitive DNA detection

Nanostructured and nanopatterned gold surfaces: application to the surface-enhanced Raman spectroscopy

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