Determination of some banned stimulants in sports by micellar liquid chromatography

Gil-Agustı́, Mayte; Capella‐Peiró, María‐Elisa; Martinavarro-Domı́nguez, Adria; Esteve‐Romero, Josep

doi:10.1007/bf02497477

Cited by 25 publications

(6 citation statements)

References 20 publications

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“…Micellar liquid chromatography (MLC) is a rather uncommon mode of reversed‐phase high‐performance liquid chromatography (RP‐HPLC) that employs aqueous solution of surfactants above the critical micellar concentration as mobile phase. Micellar mobile phases have several advantages over conventional hydro‐organic mobile phases, for example low cost, low toxicity, low volatility, the possibility of simultaneous separation of ionic and nonionic compounds without the need of gradient elution, and direct injection of physiological samples because of the solubility of proteins in the micelles 16–20.…”

Section: Introductionmentioning

confidence: 99%

Separation optimization of quercetin, hesperetin and chrysin in honey by micellar liquid chromatography and experimental design

Hadjmohammadi¹,

Nazari²

2010

J of Separation Science

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The chemometrics approach was applied for the separation optimization of flavonoid markers (quercetin, hesperetin and chrysin) in honey using micellar liquid chromatography (MLC). The investigated method combines SPE of flavonoids from honey using C(18) cartridge and their separation and quantification by micellar liquid chromatography. A two level full factorial design was carried out to evaluate the effect of four experimental factors including concentration of SDS, alkyl chain length of the alcohol used as the organic modifier (N), volume percentage of the organic modifier (V(m)) and volume percentage of acetic acid (AcOH) in mobile phase on analytes retention times. Experiments for analytes retention times modeling and optimization of separation were performed according to central composite design. Multiple linear regression method was used for the construction of the best model based on experimental retention times. Pareto optimal method was used to find suitable compatibility between resolution and analysis time of analytes in honey. The optimum mobile phase composition for separation and determination of analytes in honey were [SDS]=0.124 mol/L; 7.8% v/v ethanol and 5.0% v/v AcOH. Limits of detection and linear range of flavonoid markers were 0.0079-0.0126, 0.05-50.0 mg/L, respectively.

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Section: Introductionmentioning

confidence: 99%

Separation optimization of quercetin, hesperetin and chrysin in honey by micellar liquid chromatography and experimental design

Hadjmohammadi¹,

Nazari²

2010

J of Separation Science

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“…Another advantage is that the use of micellar mobile phases is non-toxic, non-flammable, biodegradable and relatively inexpensive, in comparison to aqueous-organic solvents [16]. Two selected studies are an example of using micellar mobile phases with direct injection applied to the determination of stimulants in urine [17] and barbiturates in serum [18].…”

Section: Introductionmentioning

confidence: 99%

Direct determination of verapamil in urine and serum samples by micellar liquid chromatography and fluorescence detection

Rambla-Alegre

Gil-Agustı́

Capella‐Peiró

et al. 2006

Journal of Chromatography B

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“…Furthermore, banned drugs in sport, ephedrine and norephedrine, were also detected by micellar liquid chromatography [25,26]. However, the retention time of E and norephedrine was 48.13 and 58.28, respectively, and the theoretical plate number of E and norephedrine was less than 2000.…”

Section: Introductionmentioning

confidence: 99%

Development micellar HPLC method for simultaneous determination of ephedrine, pseudoephedrine, and methylephedrine in Ephedra Herb and traditional Chinese medicinal preparations

Dong

et al. 2015

Acta Chromatographica

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Summary.A micellar high-performance liquid chromatography (HPLC) method has been described for simultaneous determination of ephedrine, pseudoephedrine, and methylephedrine in Ephedra Herb and two traditional Chinese preparations. The separation and determination of ephedrine, pseudoephedrine, and methylephedrine were performed using a mobile phase containing 1.75 × 10 −1 mol·L −1 sodium dodecyl sulphate and 0.02 mol·L −1 potassium hydrogen phosphate with 10% (v/v) methanol at pH 3.0, running at 1.5 mL·min −1 by a Venusil XBP C18 (250 × 4.6 mm, 5 μm) column at 40 °C. The detected wavelength was set at 210 nm. The method was validated according to the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines. The main analytical parameters were linearity (r > 0.9990), intra-and inter-day precisions (relative standard deviation [RSD %], 0.33-1.63, and RSD %, 1.26-2.20, respectively), limit of quantifications [LOQs], and limit of detections [LODs] (2.6 × 10 −4 and 7.8 × 10 −5 mg·mL −1 for ephedrine, 6.8 × 10 −4 and 2.0 × 10 −4 mg·mL −1 for pseudoephedrine, and 5.0 × 10 −4 and 1.5 × 10 −4 mg·mL −1 for methylephedrine). RSDs of recoveries were <5.5% in the three samples. Based on the optimized chromatographic conditions and the eluted orders, a model of separation mechanism for the analytes was established. The results indicated that the proposed method was an accurate, "green" and cheap method.

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Determination of some banned stimulants in sports by micellar liquid chromatography

Cited by 25 publications

References 20 publications

Separation optimization of quercetin, hesperetin and chrysin in honey by micellar liquid chromatography and experimental design

Separation optimization of quercetin, hesperetin and chrysin in honey by micellar liquid chromatography and experimental design

Direct determination of verapamil in urine and serum samples by micellar liquid chromatography and fluorescence detection

Development micellar HPLC method for simultaneous determination of ephedrine, pseudoephedrine, and methylephedrine in Ephedra Herb and traditional Chinese medicinal preparations

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