Direct determination of verapamil in urine and serum samples by micellar liquid chromatography and fluorescence detection

Rambla-Alegre, Maria; Gil-Agustı́, Mayte; Capella‐Peiró, María‐Elisa; Carda‐Broch, Samuel; Esteve‐Romero, Josep

doi:10.1016/j.jchromb.2006.03.054

Cited by 33 publications

(20 citation statements)

References 18 publications

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“…22 However, in this case, the addition of 1-butanol produces a too fast elution (near the dead volume), and 1-propanol does not provide any significant advantage. Therefore, no alcohol was added to the mobile phase.…”

Section: Mobile Phase Selection and Chromatographic Optimizationmentioning

confidence: 95%

Use of micellar mobile phases for the chromatographic determination of melamine in dietetic supplements

Beltrán-Martinavarro

Péris-Vicente

Marco-Peiró

et al. 2012

Analyst

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Melamine is a nitrogen-rich industrial chemical which is occasionally used to increase the apparent protein content of different products destined for human and animal consumption. In this work, a liquid chromatographic procedure that uses micellar mobile phases of sodium dodecyl sulfate (SDS) buffered at pH 3, a C18 column and UV detection is reported for the determination of melamine in dietetic supplements. Samples were reconstituted with a SDS solution and were directly injected, thus avoiding long extraction and experimental procedures. Melamine was eluted in less than 10 min with no interference by other compounds of the matrices. The optimum mobile phase composition was taken by a chemometrical approach that considers the retention factor, efficiency and peak shape. Validation was performed following the indications of the European Commission (Decision 2002/657/EC). The following parameters were considered: linearity (0.02-100 mg mL À1 ; R 2 ¼ 0.9996), intra-and inter-day precisions (<12.4%), accuracy (90.0-101.3%), and robustness (less than 9.8% and 5.1%, for retention time and peak area, respectively). The limits of detection and quantification were 9 and 20 ng mL À1 , respectively. Recoveries for several spiked samples were in the 85.8-114.3% range. These results indicate that the proposed methodology is useful for routine analysis of control quality of infant formula and adult dietetic supplements.

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Section: Mobile Phase Selection and Chromatographic Optimizationmentioning

confidence: 95%

Use of micellar mobile phases for the chromatographic determination of melamine in dietetic supplements

Beltrán-Martinavarro

Péris-Vicente

Marco-Peiró

et al. 2012

Analyst

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“…Atenoretic capsules (manufactured by Sigma Pharmaceutical Industries for Queen Pharm International) were labeled to contain 50 mg of AT, 2.5 mg of AM, and 25 mg of HZ. Urine samples were obtained from nine volunteers (25)(26)(27)(28)(29)(30) years old) administered with the ternary combined chemotherapy. All urine samples were used without any pretreatment.…”

Section: Experimental Materialsmentioning

confidence: 99%

“…Miceller liquid chromatography (MLC) was reported as a suitable technique for eco-friendly (bio)pharmaceutical analysis of drugs [29]. Miceller solution can replace conventional aqueous organic mobile phase with good results.…”

Section: Surfactants As Mobile Phase Additivesmentioning

confidence: 99%

“…They provide as well availability for the direct injection of biological fluids by solubilizing the protein components [28]. Micelles of sodium dodecyl sulphate (SDS) were used with different bonded stationary phases (mostly C8, C18, and cyanopropyl) for the quantification of compounds in biological matrices [29]. Furthermore, cyclodextrins (CDs) are seminatural compounds produced from intramolecular transglycosylation reactions during the enzymatic degradation of starch.…”

Section: Introductionmentioning

confidence: 99%

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Eco-Friendly Simultaneous Chromatographic Determination of Amiloride Hydrochloride, Atenolol, and Hydrochlorothiazide in Urine

Albishri

El‐Hady

Tayeb

2015

Acta Chromatographica

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Summary. Pharmaceutical industry concerned recently with eco-friendly analytical methods to reduce the environmental pollution. The using of toxic organic solvents for the analysis of drugs is critical. In the current work, several simple and less costly approaches such as micellar and/or cyclodextrin liquid chromatography were discussed. A new eco-friendly and simple chromatographic analysis of the ternary mixture of amiloride hydrochloride (AM), atenolol (AT), and hydrochlorothiazide (HZ) in urine by hydroxypropyl-beta-cyclodextrin (HP-β-CD) bonded stationary phase was investigated. The experimental conditions were optimized and validated based on International Conference on Harmonization (ICH) Q2R1 guidelines to detect analytes by isocratic mobile phase of phosphate buffer (5.0 mmol L −1 , pH 7.0) in the presence of 0.5 mL min −1 flow rate, 25.0 °C, and 280 nm. Linearity, accuracy, and precision were found to be acceptable over the concentration range of 0.05-20.0 μg mL −1 for AM, 0.05-50.0 μg mL −1 for AT and 0.05-50.0 μg mL −1 for HZ. The proposed method was precise, selective, and sensitive enough for the routine analysis of ternary mixture at therapeutic urine levels. The inclusion complexation and the appendant hydroxyl groups of HP-β-CD were considered the main reasons for assisting in adequate separation of the drugs. On the other hand, the presence of kosmotropic phosphate ions could solubilize the protein and could strengthen the selective inclusion of drugs inside HP-β-CD cavity. Urinary excretion studies showed that the detection of drugs is possible up to 24 h after their ingestion.

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“…8 Several high-performance liquid chromatographic (HPLC) method have been reported for the determination of verapamil in biological samples by UV detection [9][10][11] or more frequently fluorescence detection [12][13][14][15][16][17] owing to the native fluorescence properties of this compound.…”

mentioning

confidence: 99%

Method Development of Verapamil in Presence of NSAIDs using RP-HPLC Technique

Sultana¹,

Arayne²,

Waheed³

2011

Bulletin of the Korean Chemical Society

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Verapamil is a calcium channel blocker and is classified as a class IV anti-arrhythmic agent. It is used in the control of supra ventricular tachyarrhythmias, and in the management of classical and variant angina pectoris. It is also used in the treatment of hypertension and used as an important therapeutic agent for angina pectoris, ischemic heart disease, hypertension and hypertrophic cardiomyopathy. Verapamil commonly co-administered with NSAIDs (non-steroidal anti-inflammatory drugs) i.e. diclofenac sodium, flurbiprofen, Ibuprofen, mefanamic acid and meloxicam. A simple and rapid RP-HPLC method for simultaneous determination and quantification of verapamil and NSAIDs was developed and validated. The mobile phase constituted of acetonitrile: water (55:45) whose pH was adjusted at 2.7 and pumped at a flow rate of 2.0 mL min −1 at 230 nm. The proposed method is simple, precise, accurate, low cost and least time consuming for the simultaneous determination of verapamil and NSAIDs which can be effectively applied for the analysis of human serum.

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Direct determination of verapamil in urine and serum samples by micellar liquid chromatography and fluorescence detection

Cited by 33 publications

References 18 publications

Use of micellar mobile phases for the chromatographic determination of melamine in dietetic supplements

Use of micellar mobile phases for the chromatographic determination of melamine in dietetic supplements

Eco-Friendly Simultaneous Chromatographic Determination of Amiloride Hydrochloride, Atenolol, and Hydrochlorothiazide in Urine

Method Development of Verapamil in Presence of NSAIDs using RP-HPLC Technique

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