Research subject. The estimations of oil resources in Western Siberia obtained using a basin modeling of the Bazhenov formation as an oil source stratum indicated a significant deficit of its potential, calculated based on the pyrolytic kinetic parameters of catagenesis. Aim. To search for possible additional oil generating mechanisms parallel to catagenesis, thus clarifying the conceptual foundations of the modern theory of the organic origin of oil. Key points. Based on a review of literature publications, a hypothesis was formulated about the evolution of organic matter from sedimentary flow floccules to the injection of proto-oil into the microcracks of the autofluid fracturing of source rocks. In the upper unconsolidated bottom sediment layers, biofilms appear whose microbial matrix forms a basis for future kerogen. Proto-oil bitumoids appear as a by-product of biofilm microbial metabolism under the anaerobic conditions at diagenesis stage. Рroto-oil bitumoids are encapsulated and absorbed on the surfaces and inside the chambers and conducting channels of the biofilm polymer matrix (future kerogen). Local ultra-high pressure (LUHP) inside kerogen grains appears at the final “smectite – illite” transition stage of host clays, acting as a driving force for encapsulated bitumoid expulsion from kerogen grains. On the walls of microcracks, proto-oil bitumoids undergo hydropyrolysis, consuming cohesive film water and changing the surface philicity. Microphotographs and SEM images of thin sections are provided in order to illustrate LUHP-expulsion traces. Two types of oil source rocks are differentiated based on the possibility of proto-oil LUHP expulsion: those with a plastic mineral framework and a rigid mineral framework. Conclusion. The presence of porous spaces filled with bitumoids was established in the kerogen of immature potentially oil source rocks. This mass of encapsulated bitumoids is an additional resource of oil generation within the “oil window”. Their expulsion volume can be estimated and attributed to a certain temperature range. Directions for further research are outlined.