Determination of stability characteristics for electrochemical biosensors via thermally accelerated ageing

Panjan, Peter; Virtanen, Vesa; Sesay, Adama M.

doi:10.1016/j.talanta.2017.04.011

Cited by 50 publications

(35 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Glucose depletion was measured by a glucose biosensor based on a GOx enzymatic electrochemical biosensor as reported in Panjan et al [25] and its integration and operation is described in Lladó Maldonado et al [22].…”

Section: Glucose Concentrationmentioning

confidence: 99%

Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus

Maldonado

Krull

Rasch

et al. 2019

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

Bioreactors at the microliter-scale offer a promising approach to accelerate bioprocess development. Advantages of such microbioreactors include a reduction in the use of expensive reagents. In this study, a chemostat operation mode of a cuvette-based microbubble column-bioreactor made of polystyrene (working volume of 550 μL) was demonstrated. Aeration occurs through a nozzle (Ø ≤ 100 μm) and supports submerged whole-cell cultivation of Staphylococcus carnosus. Stationary concentrations of biomass and glucose were determined in the dilution rate regime ranging from 0.12 to 0.80 1/h with a glucose feed concentration of 1 g/L. For the first time reaction kinetics of S. carnosus were estimated from data obtained from continuous cultivation. The maximal specific growth rate (µ max = 0.824 1/h), Monod constant (K S = 34•10 3 g S /L), substrate-related biomass yield coefficient (Y X/S = 0.315 g CDW /g S), and maintenance coefficient (m S = 0.0035 g S /(g CDW •h)) were determined. These parameters are now available for further studies in the field of synthetic biology.

show abstract

Section: Glucose Concentrationmentioning

confidence: 99%

Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus

Maldonado

Krull

Rasch

et al. 2019

Bioprocess Biosyst Eng

View full text Add to dashboard Cite

show abstract

“…Stability time of the genosensor is an essential parameter that allows the feasibility of its commercialization. Decrease in sensitivity over time is referred to as aging of the biosensor, having a complex mechanism that affects several parameters, such as the polymer layers and biomolecules . Stability of the electrochemical genosensor was monitored (storage at 8 °C) by differential pulse voltammetry and the response was evaluated every 10 days, for two months.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical Detection of Zika Virus in Biological Samples: A Step for Diagnosis Point‐of‐care

et al. 2019

View full text Add to dashboard Cite

This work describes an electrochemical genosensor for detection of genomic RNA of Zika virus in real samples of infected patients, using a new platform based on graphite electrodes modified with electrochemically reduced graphene oxide and polytyramine‐conducting polymer. The developed genosensor was suitable for differentiation between samples of healthy and infected patients with Zika virus by differential pulse voltammetry, detecting up to 0.1 fg/mL (1.72 copies/mL), showing good stability (about 60 days), rapid analysis (about 20 min) and potential for filling the lack of practical diagnostic methods for Zika virus.

show abstract

“…The observations showed that the relative average sensitivity was maintained at 80% or above during the first 15 days. On the 22 nd day, the relative sensitivity of the biosensor was 58.27% because of the decrement of the enzyme activity and the destruction of the membrane [42]. Finally, the relative average sensitivity of the biosensor was measured again on the 29 th day and it had dropped to 26.82%.…”

Section: F Lifetime Of Mbs-nafion-gox/go/azo Glucose Biosensormentioning

confidence: 99%

Integrating a Plastic Glucose Biosensor Based on Arrayed Screen-Printed Electrodes Utilizing Magnetic Beads with a Microfluidic Device

Chou

Lin

Kuo

et al. 2019

IEEE J. Electron Devices Soc.

View full text Add to dashboard Cite

In this study, a novel potentiometric glucose biosensor based on magnetic beads-Nafionglucose oxidase/graphene oxide/aluminium-doped zinc oxide (MBs-Nafion-GOx/GO/AZO) membranes on a PET substrate with arrayed screen-printed electrodes (SPEs) was proposed. The morphologies, elemental analysis, and surface roughnesses of the sensing membranes were characterized. The sensing parameters of the biosensor were investigated by using the voltage-time (V-T) measurement system, which measured sensitivity, linearity, anti-interference ability, reversibility, temperature effect, and lifetime. A microfluidic device using polydimethylsiloxane (PDMS) was integrated with the V-T measurement system to analyze the response characteristics. The results under static solution and microfluidic flow conditions were compared. The results showed that the average sensitivity and linearity of the biosensor depended on the microfluidic flow rate. The best average sensitivity of the glucose biosensor based on MBs-Nafion-GOx/GO/AZO membranes was 20.35 mV/mM at a flow rate of 15 µL/min. INDEX TERMS Aluminium-doped zinc oxide (AZO), graphene oxide (GO), magnetic beads (MBs), glucose biosensor, screen-printed electrodes (SPEs), microfluidics.

show abstract

Determination of stability characteristics for electrochemical biosensors via thermally accelerated ageing

Cited by 50 publications

References 37 publications

Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus

Application of a multiphase microreactor chemostat for the determination of reaction kinetics of Staphylococcus carnosus

Electrochemical Detection of Zika Virus in Biological Samples: A Step for Diagnosis Point‐of‐care

Integrating a Plastic Glucose Biosensor Based on Arrayed Screen-Printed Electrodes Utilizing Magnetic Beads with a Microfluidic Device

Contact Info

Product

Resources

About