Determination of stigmasterol, β-sitosterol and stigmastanol in oral dosage forms using high performance liquid chromatography with evaporative light scattering detection

Nair, Vipin D. P.; Kanfer, Isadore; Hoogmartens, Jos

doi:10.1016/j.jpba.2005.12.030

Cited by 59 publications

(37 citation statements)

References 29 publications

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“…The most common phytosterol compounds is stigma sterol. Those compounds comprise 98% of all the vegetable sterols identified in plants [5].…”

Section: Introductionmentioning

confidence: 99%

Gc-MS Analysis of Invasive Aquatic Weed, Pistia Stratiotes L. And Eichhornia Crassipes (Mart.) Solms

Tyagi

2017

Int J Curr Pharm Sci

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Objective: To investigate the bioactive components of an invasive aquatic weed, Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms vegetative parts by using Gas Chromatography-Mass Spectrometer (GC-MS). Methods:The chemical compositions of the ethanol extract of whole plant Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms was investigated using Agilent Technologies GC-MS (GC-7890A, MS 5975C). Results:The results of GC-MS analysis of the ethanolic extract revealed the existence of 28 phytochemical compounds in Pistia stratiotes L. nHexadecanoic acid,-11-Hexadecenoic acid, ethyl ester, Hexadecanoic acid, ethyl ester, Octadecanoic acid, ethyl ester, 2-Cyclopenten-1-one, 5-hydroxy-2,3-dimethyl, L-Glutamine, 2-Pentadecanone, 6,10,14-trimethyl, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, methyl ester,(Z,Z,Z), Nonadecane, 12,15-Octadecadiynoic acid, methyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, Diisooctyl phthalate, Docosanoic acid, ethyl ester, Stigmasterol, Bis(2-ethylhexyl) phthalate, 1-Monolinoleoylglycerol trimethylsilyl ether, Ethyl iso-allocholate are the major compound.The ethanolic extract of Eichhornia crassipes (Mart.) Solms contains 43 phytochemical compounds of high and low molecular weight nHexadecanoic acid, E-11-Hexadecenoic acid, ethyl ester, Palmitic acid, Phytol, 9,12,15-Octadecatrienal, 9,12-Octadecadienoic acid, ethyl ester, Linolenic acid, ethyl ester, Stearic acid, ethyl ester, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, α-Glyceryl linolenate, 1-Monolinoleoylglycerol trimethylsilyl ether, Linoleic acid, 2,3-bis-(O-TMS)-propyl ester, Stigmasterol, Linolelaidic acid, methyl ester, 9,12,15-Octadecatrienoic acid, ethyl ester, (Z,Z,Z), Ethyl iso-allocholate, Cholesta-22,24-dien-5-ol, 4,4-dimethyl are the major compounds.These results indicates Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms possess potent antioxidant, anti-inflammatory, anticancer, antitumour, antiarthritic, cancer preventive, antibacterial effects so can be recommended as a plant of phytopharmaceutical importance. Conclusion:The ethanol extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) Solms proves as a potential source of bioactive compounds of pharmacological importance.

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“…The most common phytosterol compounds is stigma sterol. Those compounds comprise 98% of all the vegetable sterols identified in plants [5].…”

Section: Introductionmentioning

confidence: 99%

Gc-MS Analysis of Invasive Aquatic Weed, Pistia Stratiotes L. And Eichhornia Crassipes (Mart.) Solms

Tyagi

2017

Int J Curr Pharm Sci

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“…A Luna C 18 (2) (5μm, 150 x 4.6 mm ID) column (Phenomenex, Torrance, CA, USA) was used at 23 ± 2 ºC. A mobile phase consisting of acetonitrile: water in isocratic mode (20: 80, v/v) was used at a flow rate of 1 mL/min using a detection wavelength of 260 nm (24,26).…”

Section: Instrumentationmentioning

confidence: 99%

“…Although assay methods for use in the quality control (QC) of AP products have been developed and described (24)(25)(26), a further important QC criterion, namely, dissolution testing still needs to be addressed. The maintenance of the release properties required to effect consistent absorption from the gastrointestinal tract (GIT) is considered as an important feature and valuable quality control (QC) parameter.…”

Section: Introductionmentioning

confidence: 99%

“…During the analysis of some commercially available AP products it was found that BSS was the major component present in some of those AP products when compared to the content of other sterols and stanols (24). This sterol was consequently chosen as the marker for the dissolution testing of AP products with labelled content of BSS.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, hypoxoside was chosen as a marker for products which were assayed (25) and found to contain quantifiable amounts of this norlignan component. Analyses of the phytochemicals were carried out using the equipment and validated analytical methods previously reported (24,25).…”

Section: Introductionmentioning

confidence: 99%

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Development of dissolution tests for the quality control of complementary/alternate and traditional medicines: Application to African potato products

Nair

Kanfer

2008

J Pharm Pharm Sci

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-PURPOSE. Unlike orthodox medicines, specific guidelines for dissolution testing of complementary/alternate (CAMs) and traditional medicines (TMs) have not been developed nor is dissolution testing a requirement for the quality control of such products. In this report, the dissolution of African Potato (AP) products, an African traditional medicine (ATM) which has been ingested by man for a diversity of ailments, has been investigated. A norlignan glycoside namely hypoxoside and a sterol, β-sitosterol (BSS) are purported to be the most important phytochemicals in marketed products of AP. Dissolution testing of AP products containing labelled content of sterols and those containing only hypoxoside is proposed whereby BSS and hypoxoside are monitored as markers for the release of the contents of the abovementioned products, respectively. METHODS. The FDA dissolution guidance for industry was used to study the best dissolution condition for several formulations of AP. Buffers in the range of pH 1.2 to 7.5 were used to investigate the dissolution of AP products containing hypoxoside as a marker compound. Similarly, biorelevant dissolution media such as fasted state simulation fluid (FaSSIF) and fed state simulation fluid (FeSSIF) at different pH were used to investigate the release of BSS in AP formulations labelled to contain sterols which exhibited poor water solubility. RESULTS. Dissolution testing of AP products containing hypoxoside, conducted at pH 1.2 using USP Apparatus 1 indicated that more than 75% of hypoxoside was released within 1 hr. Dissolution testing of products containing sterols, conducted in FeSSIF at a pH of 5.0 resulted in a release of at least 75% of BSS after 1 hr for all but one of the products tested. CONCLUSIONS. Dissolution testing conditions have been developed for AP products containing two different marker compounds where one of the components, hypoxoside, is water soluble, whereas another component, BSS is poorly water soluble. This necessitated the use of different dissolution media and pHs in order to monitor the respective release of hypoxoside and BSS from AP products.The results of this study indicate the necessity and possibility of developing appropriate dissolution testing procedures for use in the quality control of CAMs/TMs.

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Processing Effects on Functional Components in Cereals and Grains

Yadav

Joyner

2014

Functional Foods and Dietary Supplements

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Determination of stigmasterol, β-sitosterol and stigmastanol in oral dosage forms using high performance liquid chromatography with evaporative light scattering detection

Cited by 59 publications

References 29 publications

Gc-MS Analysis of Invasive Aquatic Weed, Pistia Stratiotes L. And Eichhornia Crassipes (Mart.) Solms

Gc-MS Analysis of Invasive Aquatic Weed, Pistia Stratiotes L. And Eichhornia Crassipes (Mart.) Solms

Development of dissolution tests for the quality control of complementary/alternate and traditional medicines: Application to African potato products

Processing Effects on Functional Components in Cereals and Grains

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