Determination of streptomycin in serum by high-performance liquid chromatography

Kurosawa, Nahoko; Kuribayashi, Shoichi; Owada, Eiji; Ito, Keiji; Nioka, Masanori; Arakawa, Masako; Fukuda, Ryohei

doi:10.1016/s0378-4347(00)84606-x

Cited by 17 publications

(14 citation statements)

References 9 publications

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“…Both methods required laborious derivatization. Two other HPLC methods [4,5] with direct UV detection at 195 nm utilise acetonitrile-water (8:92, v/v) as a mobile phase. Under these circumstances the quantification limits were 500 ppb for human serum [4] and 250 ppb for assays in drugs [5].…”

Section: Resultsmentioning

confidence: 99%

“…These residues may pose a health threat to consumers, depending on the type of food and the amount of residue present. Antibiotic residues can be found in food products made from meat and their levels, must by quantified by law [2][3][4][5][6]. The Codex Committee on Residues of Veterinary Drugs determines priorities for the consideration of residues of veterinary drugs in foods and recommends Maximum Residue Limits (MRLs) for veterinary drugs.…”

Section: Introductionmentioning

confidence: 99%

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Determination of streptomycin in eggs yolk by capillary electrophoresis

Kowalski

Olędzka

Okoniewski³

et al. 1999

Chromatographia

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KeyWords Capillary electrophoresis Streptomycin Eggs yolk Maximum residue limits SummaryA capillary electrophoresis method for the determination of streptomycin in eggs is described. Analyses were performed on an uncoated silica capillary using a buffer solution of 30mM sodium dihydrophosphate, 5 mM boric acid and 5 mM sodium tetraborate. Analytes were detected at 200 nm an the calibration curve was linear over the range of0.16 to 2.0/~g g 1 (r = 0.999). The total analysis time was 7 min. The method has been successfully applied to the quantitative determination of streptomycin in hen eggs after drug ingestion and could used for evaluation of maximum residue limits.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of streptomycin in eggs yolk by capillary electrophoresis

Kowalski

Olędzka

Okoniewski³

et al. 1999

Chromatographia

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“…Streptomycin has no important chromophore group and is generally derivatized in order to obtain ultraviolet (UV)-absorbing or fluorescent compounds (Kurosawa et al, 1985;Kubo et al, 1986;Gerhardt et al, 1994).…”

Section: Methods Performancementioning

confidence: 99%

Monitoring of streptomycin and dihydrostreptomycin residual levels in porcine meat press juice and muscle via solid‐phase fluorescence immunoassay and confirmatory analysis by liquid chromatography after post‐column derivatization

Pyun

El‐Aty

Hashim

et al. 2007

Biomedical Chromatography

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A solid-phase fluorescence immunoassay (SPFIA) that was primarily developed for detection of antibiotic residues in milk was qualitatively applied for the pre-screening of the residues of aminoglycoside antibiotics, streptomycin and dihydrostreptomycin, in meat press juice. The confirmation of both analytes was performed using a validated method of highperformance liquid chromatography with post-column derivatization. The analytical performance was demonstrated by the analysis of pork meat samples spiked at three concentration levels, ranging from 0.25 to 2.5 ppm for each analyte. In general, the recoveries ranged from 80.4 to 81.5% and from 79.6 to 84.4% for streptomycin and dihydrostreptomycin, respectively, with relative standard deviations lower than 6%. The limits of detection were 0.1 and 0.15 ppm for streptomycin and dihydrostreptomycin, respectively, and the limits of quantification of 0.35 and 0.5 ppm are below the maximum residue limits of Codex, the European Union, and the Korean Food and Drug Administration (ranging from 0.5 to 0.6 ppm). Eight real samples collected from the Seoul area were first monitored using SPFIA, and none of them were found positive. These findings are in good accordance with those observed by HPLC analysis. To the best of our knowledge, this is the first report to monitor the aminoglycoside residues in pork meat press juice using SPFIA.

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“…Limits of detection (signal-to-noise ratio of 3) were 0.5 and 1.2 g/mL for STP and STD, respectively, and the limit of quantification (signal-to-noise ratio of 10) for STP was 3 and 1.9 g/mL for STD using an injection volume of 20 L. The limits of quantification reported by Whall were 2.0 and Table 2 Intraday and interday assay precision and accuracy for STP sulfate and STD sulfate in human serum (n = 3) [23]. Furthermore, our HPLC method is significantly more sensitive, requires less sample and permits the identification and measurement of STD, a metabolic derivative by enzymatic hydrolysis of STP in human serum of STP-treated patients.…”

Section: Limits Of Detection and Quantificationmentioning

confidence: 94%