Determination of Sulfide with Acidic Permanganate Chemiluminescence for Development of Deep-sea in-situ Analyzers

Fujimori, Kunihide; Tsujimoto, Kenta; Moriuchi‐Kawakami, Takayo; Shibutani, Yasuhiko; Ueda, Masato; Suzue, Takahiko; Kimoto, Hideshi; Okamura, Kei

doi:10.2116/analsci.27.183

Cited by 11 publications

(4 citation statements)

References 26 publications

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“…Further, while the research here is centered on superoxide, DISCO will be amenable to the analysis of an extensive range of chemical species that have high-sensitivity chemiluminescent probes . These include species with half-lives too short for ex situ analysis [e.g., nitric oxide (NO), adenosine triphosphate (ATP)] and species that are sensitive to even subtle changes in the redox environment [e.g., both aqueous ferrous (Fe 2+ ) and ferric (Fe 3+ ) iron, and hydrogen sulfide (H 2 S)]. ,− DISCO may also enable the measures of other inorganic [e.g., phosphate (PO 4 3+ ), copper (Cu 2+ ), cobalt (Co 2+ ), bromide (Br – ), chromium (Cr 3+ )] − and organic compounds [including key metabolites (e.g., riboflavin), contaminants, and pharmaceuticals]. Thus, this platform will open new scientific paths of exploration beyond ROS and corals, which remain out of reach by other approaches (e.g., mass spectrometers and electrochemical sensors) and will thereby pave the way for numerous applications for measuring chemical species within shallow ecosystems in situ.…”

Section: Resultsmentioning

confidence: 99%

Development of a Handheld Submersible Chemiluminescent Sensor: Quantification of Superoxide at Coral Surfaces

Grabb

Kapit

Wankel

et al. 2019

Environ. Sci. Technol.

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Reactive oxygen species (ROS) are produced via various photochemical, abiotic, and biological pathways. The low concentration and short lifetime of the ROS superoxide (O2 •–) make it challenging to measure in natural systems. Here, we designed, developed, and validated a DIver-operated Submersible Chemiluminescent sensOr (DISCO), the first handheld submersible chemiluminescent sensor. The fluidic system inside DISCO is controlled by two high-precision pumps that introduce sample water and analytical reagents into a mixing cell. The resultant chemiluminescent signal is quantified by a photomultiplier tube, recorded by a miniature onboard computer and monitored in real time via a handheld underwater LED interface. Components are contained within a pressure-bearing housing (max depth 30 m), and an external battery pack supplies power. Laboratory calibrations with filtered seawater verified instrument stability and precision. Field deployment in Cuban coral reefs quantified background seawater-normalized extracellular superoxide concentrations near coral surfaces (0–173 nM) that varied distinctly with coral species. Observations were consistent with previous similar measurements from aquaria and shallow reefs using a standard benchtop system. In situ quantification of superoxide associated with corals was enabled by DISCO, demonstrating the potential application to other shallow water ecosystems and chemical species.

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Section: Resultsmentioning

confidence: 99%

Development of a Handheld Submersible Chemiluminescent Sensor: Quantification of Superoxide at Coral Surfaces

Grabb

Kapit

Wankel

et al. 2019

Environ. Sci. Technol.

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“…The CL intensities were measured using an EN‐21E CL analyser (Kimoto Electric Co., Ltd., Japan) fitted with an H6780–06 photomultiplier tube (λ = 185–650 nm; Hamamatsu Photonics, Japan) and an autofilter changer (FC8F‐25A; Asahi Spectra Co. Ltd., Japan). A scroll CL reaction cell (quartz glass, diameter: 3 cm, ID: 1.3 mm, volume: 0.23 mL) was set up inside the analyser as reported previously . Tygon tubing (LMT‐55; ID: 1.59 × 3.18 mm; Saint‐Gobain K.K., France) and a peristaltic pump (MPe; Tokyo Rikakikai Co. Ltd., Japan) were used to transport the solutions into the analyser.…”

Section: Methodsmentioning

confidence: 99%

“…A scroll CL reaction cell (quartz glass, diameter: 3 cm, ID: 1.3 mm, volume: 0.23 mL) was set up inside the analyser as reported previously. [22] Tygon tubing (LMT-55; ID: 1.59 × 3.18 mm; Saint-Gobain K.K., France) and a peristaltic pump (MPe; Tokyo Rikakikai Co. Ltd., Japan) were used to transport the solutions into the analyser. The pump was connected to the solution vessels and CL reaction cell, and drainage was provided by Teflon tubing (Iwase Co., Japan).…”

Section: Apparatus and Proceduresmentioning

confidence: 99%

Enhanced chemiluminescence for trazodone trace analysis based on acidic permanganate oxidation in concurrent presence of rhodamine 6G

et al. 2017

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A new sensitized chemiluminescence method by acidic permanganate oxidation was developed for the sensitive determination of trazodone. A fluorescent dye as used rhodamine 6G to increase a chemiluminescence intensity. Under optimum conditions, the liner range of the calibration curve was obtained for 1-5000 nmol/L. The limit of detection was calculated from 3σ of a blank was 0.23 nmol/L. The coexistent ions and substances had no interference with the chemiluminescence measurement. The chemiluminescence spectra were measured to elucidate a possible mechanism for the system. The present method was satisfactorily used in the determination of the drugs in pharmaceutical samples and animal serums.

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“…Further, while the research here is centered on superoxide, DISCO will be amenable to analysis of an extensive range of chemical species that have high sensitivity chemiluminescent probes (Fletcher et al, 2001). These include species with half-lives too short for ex situ analysis (e.g., nitric oxide (NO), adenosine triphosphate (ATP)) and species that are sensitive to even subtle changes in the redox environment (e.g., both aqueous ferrous (Fe 2+ ) and ferric (Fe 3+ ) iron, and hydrogen sulfide (H 2 S) (Bowie et al, 2002;Safavi, 2002;King et al, 2007;Fujimori et al, 2011). DISCO may also enable measures of other inorganic (e.g., phosphate (PO 3− 4 ), copper (Cu 2+ ), cobalt (Co 2+ ), bromide (Br − ), chromium (Cr 3+ )) (Coale et al, 1992;Escobar et al, 1993;Rehman et al, 2010) and organic compounds (including key metabolites (e.g., riboflavin), contaminants, and pharmaceuticals).…”

Section: Future Research Within and Beyond Corals And Rosmentioning

confidence: 99%

Exploring the role of reactive oxygen species (ROS) in marine ecosystem health and function

Grabb¹

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With the rapid decline of coastal ecosystems such as coral reefs and seagrasses, it is crucial to better understand the health of these ecosystem to prevent future loss. Reactive oxygen speices (ROS), such as superoxide and hydrogen peroxide, play an underappreciated role in both organism health and ecosystem biogeochemical cycles. This thesis lays the foundation to measure and identify ROS production by coral in situ and through genomic analysis while also highlighting the important role that ROS can play within biogeochemical cycling within seagrass ecosystems. To measure in situ extracellular superoxide, we develop the first DIver-operated Submersible Chemiluminescent sensOr (DISCO), enabling high resolution, non-invasive measurements in real time. We further refine DISCO by making it more compact, user-friendly, adaptable, and robust, enabling measurements of superoxide across a diversity of environments. Using DISCO, I observe species-specific variation in extracellular superoxide concentrations associated with healthy coral. Despite these variations across species, bioinformatic analysis of coral proteins reveal that nearly all coral species have the extracellular superoxide-producing enzyme NADPH oxidase (NOX), and thus the genetic potential to produce extracellular superoxide. This suggests that coral species likely exhibit differential NOX regulation and expression as a function of physiological responses to external stressors, which may play a role in coral immunity. I then turn to seagrass ecosystems, where I observe rapid hydrogen peroxide production and decay through predominantly reductive pathways. This has implications on the environmental redox state and biogeochemical cycling, impacting the ecosystem services that seagrasses provide to marine environments and coastal communities. Overall, this thesis highlights the potential role that ROS may be playing in organism and ecosystem health and lays the groundwork to further develop ROS as a tool to protect these coastal ecosystems against further degradation.

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Determination of Sulfide with Acidic Permanganate Chemiluminescence for Development of Deep-sea in-situ Analyzers

Cited by 11 publications

References 26 publications

Development of a Handheld Submersible Chemiluminescent Sensor: Quantification of Superoxide at Coral Surfaces

Development of a Handheld Submersible Chemiluminescent Sensor: Quantification of Superoxide at Coral Surfaces

Enhanced chemiluminescence for trazodone trace analysis based on acidic permanganate oxidation in concurrent presence of rhodamine 6G

Exploring the role of reactive oxygen species (ROS) in marine ecosystem health and function

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