Determination of T‐2 toxin in grain and grain products by HPLC and TLC

Abstract

The purpose of this study was to investigate the T-2 toxin contaminated grain and grain products consumed especially by Turkish population. The T-2 toxin was detected using the high performance liquid chromatography (HPLC) with UV detector at 208 nm and the identify of T-2 was further confirmed by thin layer chromatography (TLC). The recovery was 91 +/- 4.24% for corn flour fortified with the known amount of T-2 toxin (1 ppm). The detection limits of T-2 toxin for the HPLC and the TLC were 25 ng and 50 ng, respectively. A total of 30 commercially available grain and grain product samples were analyzed. Two corn flour samples were found to contain detectable levels of T-2 toxin at a level of 1.60 ppm and 4.08 ppm.

Mycotoxigenic moulds and mycotoxins in flours consumed in Turkey

“…Our results are confirmed by previous studies showing that grains and grain‐based foods from Turkey are contaminated by AFs and OTA . There is also substantial information on the incidence of mycotoxins in different food commodities from Turkey . However, studies on AF and OTA concentrations in flour products are limited and this study has presented comprehensive data on mycotoxin problems in different grain‐based flours from Turkey.…”

Determinatıon of ochratoxin A and total aflatoxin levels in corn samples from Turkey by enzyme-linked immunosorbent assay

“…Moreover, it is also a major livestock feed ingredient. There is substantial information on the incidence of mycotoxins in different food commodities in Turkey (Aycicek et al 2002;Baydar et al 2005;Giray et al 2007;Gokmen and Acar 2000;Nizamlıoglu and Oguz 2003;Omurtag and Beyoglu 2003;Omurtag and Yazicioglu 2000;Simsek and Bozkurt 1992). However, studies on AF and OTA concentrations in corn and corn products (Baydar et al 2005;Alp et al 1997;Karagozlu and Karapinar 2000;Nizamlioglu and Oguz 2003;Sevikturk and Gonulalan 2007) are limited and only restricted to those for animal consumption, with data indicating low contamination levels.…”

Occurrence of T-2 toxin in processed cereals and pulses in Turkey determined by HPLC and TLC

“…For the mycotoxins T-2 toxin, fumonisins B1 and B2 and the a¯atoxins a guideline appears only for the latter in Turkey (Omurtag et al 1998, Omurtag 2001. There is also a lack of legislation on the acceptable levels of TCTCs in crop products since there are no limits for the TCTC toxins except for deoxynivaleno l (Canada, Russia, and the United States) (Stratton et al 1993).…”