Determination of Tadalafil in Pure Powder and Tablet Dosage Form by High-Performance Liquid Chromatography

Barot, Tushar G; Patel, P. K.

doi:10.1093/jaoac/93.2.516

Cited by 9 publications

(4 citation statements)

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“…Analysis of Tadalafil from pharmaceutical preparations using UV spectrophotometry [17][18][19][20][21][22][23][24][25][26] and 1 th Degree Derivative Spectrophotometry [27,28] are reported in the literature as spectroscopic analysis. In terms of chromatographic analysis; HPLC determinations from food and conventional pharmaceutics [29][30][31][32][33][34] , from human plasma and serum [35,38] and from pharmaceutics [37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53] are also took place. To the authors' knowledge, experimental pKa values for CAB and TAD are not reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometry, potentiometry and HPLC in determination of acidity constant for Cabergoline and Tadalafil

Polat¹,

Doğan²,

Başcı³

2019

jrp

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Acidic dissociation constant (pKa) is an important physicochemical parameter in absorption, dissociation and elimination mechanisms of drugs in body. Various analytical methods are utilized for the determination of pKa values of pharmaceutical active ingredients, and potentiometry, spectrophotometry and HPLC are the most common methods. Cabergoline is dopaminergic ergoline derivative having a powerful and long-term prolactin reducing effect, which is used for the treatment of Parkinson disease. Tadalafil leads an increasing cGMP level in Corpus cavernous, during secretion of nitric oxide in sexual arousal. In the presented study, detection of pKa values for Cabergoline and Tadalafil by using potentiometry, spectrophotometry and HPLC was investigated. The pKa value for Cabergoline was respectively found to be 6.42, 6.05 and 6.20 by spectrophotometry, potentiometry and HPLC. Spectrophotometric pKa value was significantly different (p>0.05) from others, and potentiometry and spectrophotometry were appropriate for pKa value determination of Cabergoline. The pKa values for Tadalafil by potentiometry and spectrophotometry were found to be 3.52 and 3.44, respectively. But, in HPLC, no differentiation was observed in retention times of Tadalafil by increasing pH value of mobile phase. Developed methods for determination of pKa values for Cabergoline and Tadalafil demonstrated high repeatability values (RSD<%1). In this study, experimental pKa values for Cabergoline and Tadalafil from developed methods were compared with the values calculated by the common softwares and high-level divergences were observed.

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Section: Introductionmentioning

confidence: 99%

Spectrophotometry, potentiometry and HPLC in determination of acidity constant for Cabergoline and Tadalafil

Polat¹,

Doğan²,

Başcı³

2019

jrp

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“…Few reports for the determination of VARD in pure, tablet dosage forms and biological fluids have been developed with the help of a variety of analytical tools including high performance liquid chromatography (HPLC) [3][4][5][6][7][8][9][10][11][12] , gas chromatography 13,14 , capillary electrophoresis 15,16 , electrochemical methods 17,18 and atomic emission spectrometry [19][20][21] . Several analytical methods have been reported for the estimation of TDF in biological fluids or pharmaceutical dosage forms include HPLC [22][23][24][25][26][27][28][29][30][31][32][33][34] , liquid chromatography-tandem mass spectrometry with electrospray ionization [35][36][37] , micellar electro kinetic capillary chromatography 38 and atomic emission spectrometry 20,21 .…”

Section: Introductionmentioning

confidence: 99%

Sensitive Spectrophotometric Assay of Two Phosphodiesterase Type 5-Inhibitors in Pure and Dosage forms Using Potassium Permanganate

2016

Chem Sci Trans

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Rapid, simple and sensitive and validated spectrophotometric methods have been developed for the assay of two phosphodiesterase type 5-inhibitors; vardenafil HCl (VARD) and tadalafil (TDF) in pure and dosage forms. The proposed methods were based on the oxidation of both drugs by a known excess of potassium permanganate (KMnO 4) in acidic medium and estimating the unreacted permanganate by the reaction with a fixed amount of three dyes, amaranth, indigo carmine and methylene blue, in the same acid medium followed by measuring the absorbance at λ max =520, 610 and 664 nm, respectively. Different variables affecting the reaction were studied and optimized. The beer's law is obeyed in the concentration ranges of 2.0-12, 2.0-15 and 2.0-12 µg mL-1 for VARD and 2.0-15, 2.0-20 and 2.0-12 µg mL-1 for TDF using amaranth, indigo carmine and methylene blue methods, respectively with a correlation coefficient ≥ 0.9992. The apparent molar absorptivity values are in the range 2.0956×10 4 , 1.2138×10 4 and 1.7502×10 4 L mol-1 cm-1 for VARD and 1.0769×10 4 , 0.7922×10 4 and 1.0918×10 4 L mol-1 cm-1 for TDF, using amaranth, indigo carmine and methylene blue methods, respectively. The limits of detection and quantification are reported. Intra-day and inter-day accuracy and precision of the methods have been evaluated. No interference was observed from the common tablet excipients. The methods were successfully applied to the assay of VARD and TDF in tablet preparations and the results were statistically compared with those of the reported methods by applying Student's t-test and F-test. The reliability of the methods was further ascertained by performing recovery studies using the standard addition method.

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“…The chemical structure of vardenafil HCl (VARD) and tadalafil (TDF) Few reports for the determination of VARD in pure, tablet dosage forms and biological fluids have been developed with the help of a variety of analytical tools including high performance liquid chromatography (HPLC) [3][4][5][6][7][8][9][10][11][12] , gas chromatography 13,14 , capillary electrophoresis 15,16 , electrochemical methods 17,18 and atomic emission spectrometry [19][20][21] . Several analytical methods have been reported for the estimation of TDF in biological fluids or pharmaceutical dosage forms include HPLC [22][23][24][25][26][27][28][29][30][31][32][33][34] , liquid chromatography-tandem mass spectrometry with electrospray ionization [35][36][37] , micellar electrokinetic capillary chromatography 38 and atomic emission spectrometry 20,21 .…”

Section: Introductionmentioning

confidence: 99%

Simple Spectrophotometric Methods for the Determination of Two Phosphodiesterase Type 5-Inhibitors in Pure and Tablets Dosage Forms Using N-Bromosuccinimide as an Oxidant

2016

Chem Sci Trans

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Three simple, sensitive spectrophotometric methods were proposed for the quantification of two phosphodiesterase type 5-inhibitors; vardenafil HCl (VARD) and tadalafil (TDF) in pure forms as well as in tablets dosage forms. The methods use N-bromosuccinimide (NBS) as an analytical reagent and three dyes, amaranth methylene blue, and indigocarmine or orange G, as auxiliary reagents. The three methods are based on oxidation reaction of VARD or TDF with a known excess of N-bromosuccinimide (NBS) in acid medium, followed by determination of unreacted NBS by the reaction with a fixed amount of three dyes, amaranth, methylene blue and indigocarmineor orange G followed by the measurement of the absorbance at 520, 664 and 610 or 478 nm, respectively. Under the optimum conditions, the three methods are applicable over the concentration ranges of 1.0-16, 1.0-12 and 1.0-10 µgmL-1 for VARD using amaranth methylene blue and indigocarmine methods, respectively and 2.0-12, 2.0-15 and 1.0-10 µgmL-1 for TDF using amaranth methylene blue and orange G, respectively. The molar absorptivities, Sandell's sensitivity values, correlation coefficients, limits of detection and quantification are reported. Intra-day and inter-day accuracy and precision of the methods have been evaluated. No interference was observed from the common tablet excipients. The methods were successfully applied to the assay of VARD and TDF in tablets preparations and the results were statistically compared with those of the reference methods by applying Student's t-test and F-test. The reliability of the methods was further ascertained by performing recovery studies using the standard addition method.

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Determination of Tadalafil in Pure Powder and Tablet Dosage Form by High-Performance Liquid Chromatography

Cited by 9 publications

References 0 publications

Spectrophotometry, potentiometry and HPLC in determination of acidity constant for Cabergoline and Tadalafil

Spectrophotometry, potentiometry and HPLC in determination of acidity constant for Cabergoline and Tadalafil

Sensitive Spectrophotometric Assay of Two Phosphodiesterase Type 5-Inhibitors in Pure and Dosage forms Using Potassium Permanganate

Simple Spectrophotometric Methods for the Determination of Two Phosphodiesterase Type 5-Inhibitors in Pure and Tablets Dosage Forms Using N-Bromosuccinimide as an Oxidant

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