Determination of Tetracycline and Streptomycin in Mixed Fungicide Products by Capillary Zone Electrophoresis

Abstract

A method of capillary zone electrophoresis (CZE) was used to determine tetracycline and streptomycin content in commercial agriculture products. The results indicated that this method was capable of analyzing the mixed fungicide in formulated products with instrument detection limit (IDL) of 0.50 microg/mL and a method detection limit (MDL) of 0.52 microg/mL for tetracycline, and IDL of 1.00 microg/mL and MDL of 1.22 microg/mL for streptomycin. Precision expressed by relative standard deviation (RSD) ranged from 1.44 to 4.37% of tetracycline and 1.00 to 4.20% of streptomycin. Recoveries were in the region of 98.2-102.5% for tetracycline and 95.3--103.0% for streptomycin. The low detection limit, the low RSD values, and the high percentage of recovery confirmed that the CZE technique is a sensitive and selective method. And the CZE method can analyze both tetracycline and streptomycin at the same time without complicated extraction and further derivative reaction.

Analysis of antibiotics by capillary electrophoresis

“…Three different brands of 10% wettable powder were tested, and all three were declared to contain 1.0% tetracycline and 9.0% streptomycin. Using the CE method, all three brands were found to contain between 0.8% and 1.1% tetracycline, with recoveries between 98% and 103% [1]. The same products, however, only contained between 3.5% and 3.8% streptomycin, well outside official limits, with recoveries between 95% and 103% [1].…”

“…Products containing streptomycin and tetracycline have a variety of applications within the agricultural industry [1]. A 0.1 M phosphate buffer at pH 2.5 was used for the separation of these antibiotics, with detection at 195 nm.…”

“…The limits of detection (LODs) were 0.50 mg/mL for tetracycline and 1.0 mg/mL for streptomycin. Using standard calibration curves of 10-50 mg/mL for tetracycline, and 50-250 mg/mL for streptomycin, commercial products, in the form of wettable powders, were analyzed [1]. Kanamycin was derivatized with o-phthalaldehyde (OPA) and mercaptoacetic acid (MAA) prior to injection, in order to separate and detect a number of related compounds [2].…”

“…CE was applied to the analysis of oxytetracycline in pig tissue samples [44]. The combination of tetracycline and streptomycin in agricultural products [1] was discussed in Section 2.1. The analysis of oxytetracycline in the presence of hydrocortisone and other corticosteroids and antibacterials [25] was discussed in Section 2.8.…”

“…Using a 0.1 M phosphate buffer at pH 2.5, wettable powders containing the combination of streptomycin and tetracycline were analyzed [1]. Three different brands of 10% wettable powder were tested, and all three were declared to contain 1.0% tetracycline and 9.0% streptomycin.…”