Determination of the active transport of fucoidan derived from Okinawa Mozuku across the human intestinal Caco-2 cells as assessed by size-exclusion chromatography

Nagamine, Takahiko; Hayakawa, Kou; Nakazato, Kyoumi; Iha, Masahiko

doi:10.1016/j.jchromb.2015.05.026

Cited by 13 publications

(11 citation statements)

References 15 publications

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“…The serum profiles after fucoidan administration varied among patients. This result was consistent with our previous findings (16,35). Since subjects continued with the same medicine(s) as before, the potential pharmacokinetic interactions between fucoidan and medicines should be clarified in the future.…”

Section: Discussionsupporting

confidence: 92%

Activation of NK cells in male cancer survivors by fucoidan extracted from Cladosiphon okamuranus

et al. 2019

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Cancer survivors are highly motivated to seek information about the use of dietary supplements and complementary nutritional therapies to improve their quality of life. Fucoidan, a sulfated polysaccharide extracted from brown marine alga, exhibits a wide range of bioactivities, including anticancer activity. As natural killer (NK) cells serve an important role in defenses against tumor cells, the present study examined the effects of fucoidan extracted from Cladosiphon Okamuranus on NK cell activity in cancer survivors. A prospective, open-label clinical study was conducted on cancer survivors treated with fucoidan via oral administration; 11 cancer survivors with a performance status of 0 or 1 participated and consumed 3 g of fucoidan for 6 months. No significant changes were observed in the mean activities of NK cells in total subjects following the ingestion of fucoidan. An analysis of each sex revealed that NK cell activity was significantly increased by the ingestion of fucoidan in male, yet not female subjects. Serum fucoidan levels were markedly increased following the ingestion of fucoidan and the peak levels ranged between 30 and 198 ng/ml. Tumor markers remained within the reference range during the trial period in subjects, in whom primary tumors were eradicated by treatment. The basal values of tumor markers were elevated in three cases; tumor markers were increased in two cases and decreased in one by the ingestion of fucoidan. These findings suggest that fucoidan enhances the activation of NK cells in male cancer survivors.

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Section: Discussionsupporting

confidence: 92%

Activation of NK cells in male cancer survivors by fucoidan extracted from Cladosiphon okamuranus

et al. 2019

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“…The uptake of fucoidan through the intestinal tract seemed to be low, but was measurable by our ELISA method (18). The transport of fucoidan across Caco-2 cells was demonstrated in a dose-dependent manner (21,22) and reached a maximum after 1 h, followed by a rapid decrease. Immunohistochemical staining revealed that fucoidan accumulated in jejunal macrophages and hepatic Kupffer cells in fucoidan-treated rats (22).…”

Section: Discussionmentioning

confidence: 80%

Detection of Fucoidan in Urine after Oral Intake of Traditional Japanese Seaweed, <i>Okinawa mozuku</i> (<i>Cladosiphon okamuranus</i> Tokida)

Tokita

Hirayama

Nakajima

et al. 2017

J Nutr Sci Vitaminol

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Summary Seaweed has been considered an indigestible food. Fucoidan, which is found abundantly in seaweed, especially in Cladosiphon okamuranus (Okinawa mozuku), has a high molecular weight and has been long believed to be hardly absorbed in the human digestive system due to a lack of certain digestive enzymes. We previously reported that fucoidan can be detected in serum and urine after oral intake of purified fucoidan in humans and rats. However, it is unclear whether the fucoidan in mozuku can be absorbed after digestion of mozuku. Therefore, we attempted to detect fucoidan in urine before and after mozuku intake. We determined the fucoidan concentration in urine after oral intake of Okinawa mozuku and urinary fucoidan was detected in several volunteers. In conclusion, these results suggest that fucoidan in mozuku can be absorbed after ingestion of mozuku.

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“…The recent work by Nagamine et al [ 19 ] uses a chromatography method in which size exclusion columns are used, along with UV absorbance detection, to analyze samples. The peaks observed as the fucoidan elutes from the column can differentiate it from other compounds, providing the components of the biological system are known.…”

Section: Methods For Quantifying and Identifying Fucoidan In Biolomentioning

confidence: 99%

“…Histology using an antibody to the fucoidan indicated presence in the small intestine, jejunal epithelial cells, mononuclear cells in lamina propria and in sinusoidal non-parenchymal cells of the liver. In nitrosamine-enhancer-fed rats, fucoidan was found in Kupffer cells (specialised tissue macrophages that line blood vessels in the liver and function as scavengers, filtering out pathogens and raising immune responses) The same research group demonstrated transport of fucoidan across Caco2 cell monolayers in vitro and urinary excretion in a human after ingestion of Cladosiphon okamuranus fucoidan [ 19 ]. The fucoidan concentration was measured using a chromatography method, in which the fucoidan was excluded from the column and appears as an early peak.…”

Section: Bioavailability: Uptake and Distribution Of Fucoidanmentioning

confidence: 99%

Therapies from Fucoidan: An Update

Fitton¹,

Stringer²,

Karpiniec³

2015

Marine Drugs

338

236

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Fucoidans are a class of sulfated fucose-rich polysaccharides found in brown marine algae and echinoderms. Fucoidans have an attractive array of bioactivities and potential applications including immune modulation, cancer inhibition, and pathogen inhibition. Research into fucoidan has continued to gain pace over the last few years and point towards potential therapeutic or adjunct roles. The source, extraction, characterization and detection of fucoidan is discussed.

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Determination of the active transport of fucoidan derived from Okinawa Mozuku across the human intestinal Caco-2 cells as assessed by size-exclusion chromatography

Cited by 13 publications

References 15 publications

Activation of NK cells in male cancer survivors by fucoidan extracted from Cladosiphon okamuranus

Activation of NK cells in male cancer survivors by fucoidan extracted from Cladosiphon okamuranus

Detection of Fucoidan in Urine after Oral Intake of Traditional Japanese Seaweed, <i>Okinawa mozuku</i> (<i>Cladosiphon okamuranus</i> Tokida)

Therapies from Fucoidan: An Update

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