Determination of the Antioxidant Activity of Prasman Leaf Extracts (Ayapana Triplinervis [Vahl]) and the Total Flavonoid and Phenol Contents of the Most Active Extracts

Yunitrianti,; Elya, Berna; Noviani, Arikadia

doi:10.22159/ijap.2020.v12s1.ff021

Cited by 3 publications

(2 citation statements)

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“…The phytomolecule(s) such as alkaloid, tannin, phenol, flavonoid, and saponin were present or not in hydromethanolic extract of leaves of A . triplinervis was evaluated qualitatively according to the protocols (Closa & Folch‐Puy, 2004; Yunitrianti et al., 2020) described below.…”

Section: Methodsmentioning

confidence: 99%

Gastroprotective effect of hydromethanolic extract of Ayapana triplinervis leaves on indomethacin‐induced gastric ulcer in male Wistar rats

et al. 2021

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This study investigated the gastroprotective effect of Ayapana triplinervis leaves against indomethacin‐induced gastric ulcer in male albino rat. Gastric ulceration was developed by single oral dose of indomethacin (30 mg/kg). Experimental rats were pretreated with omeprazole (positive control 20 mg/kg), hydromethanolic extract of A. triplinervis (200 mg/kg) for 28 days just before the indomethacin treatment. Free acidity, total acidity, pepsin activity and gastric volume, gastric pH, tumor necrosis factor‐alpha (TNF‐α), interleukin‐6 (IL‐6) in stomach and serum prostaglandin E2 levels were assessed in control, ulcerated group and A. triplinervis pretreated groups. Oxidative stress biomarkers, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in serum also evaluated. All the parameters were deviated from control in indomethacin‐treated group but significantly protected in A. triplinervis–pretreated group. The active ingredient(s) present in the A. triplinervis have antioxidant and gastroprotective properties that prevent the indomethacin‐induced gastric ulcer. Practical applications A. triplinervis has been widely consumed from earlier time as traditional medicine for the treatment of gastric problem and gastric ulcer in India. This is the first report that hydromethanolic extract of A. triplinervis has potent therapeutic properties against gastric ulcer. This work will provide a clue to the pharmaceutical industry to develop an effective gastroprotective agent.

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Section: Methodsmentioning

confidence: 99%

Gastroprotective effect of hydromethanolic extract of Ayapana triplinervis leaves on indomethacin‐induced gastric ulcer in male Wistar rats

et al. 2021

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“…Reaksi tersebut disertai perubahan warna dari ungu tua menjadi kuning muda (Alisi et al, 2018). Derajat perubahan warna menunjukkan potensi radikal bebas dari sampel/antioksidan (Mirfat et al, 2016) (Mirfat et al, 2016;Yunitrianti et al, 2020). (Norliyanti et al, 2018) dan fraksi etil asetatnya (Khairiah et al, 2018).…”

Section: Kadar Total Flavonoidunclassified

FENOL-FLAVONOID DAN AKTIVITAS ANTIOKSIDAN FRAKSI AIR DAN ETIL ASETAT DARI DAUN BINJAI (Mangifera caesia Jack. Ex. Wall)

Ramadhan

Sayakti

Ulya

et al. 2022

jiphar

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Binjai (Mangifera caesia Jack. Ex. Wall) adalah tanaman endemik Kalimantan yang berpotensi antioksidan. Penggunaan metanol sebagai pelarut dalam ekstraksi daun Binjai menghasilkan aktivitas antioksidan yang sangat kuat dibanding penggunaan etanol. Tujuan penelitian untuk mengetahui aktivitas antioksidan, kadar total fenol dan flavonoid pada fraksi etil asetat dan fraksi air dari ekstrak metanol daun Binjai. Daun binjai diekstraksi dengan metanol menggunakan soxhlet dan selanjutnya difraksinasi. Aktivitas antioksidan secara kualitatif dan kuantitatif diuji dengan metode DPPH (2,2-difenil-1-pikrilhidrazil). Kadar total fenol dan flavonoid ditentukan secara spektrofotometri menggunakan reagen Folin-Ciocalteu dan AlCl3. Hasil penelitian menunjukkan fraksi air (782 Â± 0,983 mg GAE/g fraksi) memiliki kadar total fenol lebih tinggi dibandingkan fraksi etil asetat (430 Â± 0,288 mg GAE/g fraksi). Sedangkan kadar total flavonoid fraksi etil asetat (274,32 Â± 0,082 mg QE/g fraksi) lebih tinggi dari fraksi air (118,8 Â± 0,013 mg QE/g fraksi). Aktivitas antioksidan dengan Kromatografi Lapis Tipis pada kedua fraksi menghasilkan noda kuning berlatarbelakang ungu setelah disemprot DPPH. Fraksi etil asetat menunjukkan aktivitas peredaman DPPH yang sangat kuat (IC50 = 5,356 ppm) dibandingkan fraksi air (IC50 = 23,175 ppm). Kesimpulannya yaitu fraksi etil asetat dan fraksi air dari ekstrak metanol daun Binjai menghasilkan aktivitas antioksidan yang sangat kuat dapat dikembangkan menjadi obat tradisional.

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Total Flavonoid, Total Phenolic contents and Antioxidant activity of Methanol and n-hexane extract from purple passion fruit peel

Ngibad

Herawati

Aisyah

et al. 2023

RJPT

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The utilization of passion fruit (Passiflora edulis f. edulis Sims) is still focused on the fruit, while the passion fruit peel is still not widely applied. The passion fruit peel has the potential as an antioxidant. The polarity of solvents used in the extraction process can affect the antioxidant activity of a medicinal plant. This study aims to determine in vitro antioxidant activity using DPPH and total flavonoid and phenol content of methanol and n-hexane extract from purple passion fruit peel. The powder of purple passion fruit peel was extracted using the maceration method with methanol and n-hexane solvent and a ratio of 1:12. The total flavonoids and phenols from each extract were determined using UV-Vis spectrophotometry. The in vitro antioxidant activity was performed using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) method by determining inhibitory concentration (IC50) values. The results showed that methanol and n-hexane extract yields were 4.53% and 0.76%, respectively. Furthermore, the total phenolic content of 35.95mg GA E/g extract in purple passion fruit peel was higher than the total flavonoid content of 3.25mg QE/g extract. There was an absence of total flavonoids and total phenols in n-hexane extracts. The antioxidant activity assay using the DPPH method based on IC50 values showed that methanol extract (14.63mg/L) had better antioxidant activity than n-hexane extract (37.39mg/L). In conclusion, the results suggest that methanol and n-hexane extract from Passiflora edulis f. edulis Sims can decrease free radicals and could be used as a natural antioxidant.

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Determination of the Antioxidant Activity of Prasman Leaf Extracts (Ayapana Triplinervis [Vahl]) and the Total Flavonoid and Phenol Contents of the Most Active Extracts

Cited by 3 publications

References 12 publications

Gastroprotective effect of hydromethanolic extract of Ayapana triplinervis leaves on indomethacin‐induced gastric ulcer in male Wistar rats

Gastroprotective effect of hydromethanolic extract of Ayapana triplinervis leaves on indomethacin‐induced gastric ulcer in male Wistar rats

FENOL-FLAVONOID DAN AKTIVITAS ANTIOKSIDAN FRAKSI AIR DAN ETIL ASETAT DARI DAUN BINJAI (Mangifera caesia Jack. Ex. Wall)

Total Flavonoid, Total Phenolic contents and Antioxidant activity of Methanol and n-hexane extract from purple passion fruit peel

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