Determination of the complete genome sequence of infectious hematopoietic necrosis virus (IHNV) Ch20101008 and viral molecular evolution in China

Jia, Peng; Zheng, Xuelian; Shi, Xiujie; Kan, Shihai; Wang, Jinjin; He, Jianping; Zheng, Wei; Yu, Li; Lan, Wensheng; Qun-yi, Hua; Liu, Hong; Jin, Ningyi

doi:10.1016/j.meegid.2014.08.013

Cited by 23 publications

(17 citation statements)

References 22 publications

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“…According to the World Animal Health Information Database (WAHID—OIE), authorities from Sweden, Norway and Iraq have notified IHN outbreaks in their countries to the OIE (http://www.oie.int/wahis). A number of publications could already demonstrate the importance of trade in the spread of IHN (Cieslak et al, ; Dixon, Paley, Alegria‐Moran, & Oidtmann, ; Enzmann et al, ; Enzmann, Kurath, Fichtner, & Bergmann, ; Haenen et al, ; Jia et al, ; Oidtmann, Pearce et al, ; Oidtmann, Peeler et al, ; Yoshimizu, ). Germany plays a central role in trade of rainbow trout because of its geographical position in Europe.…”

Section: Discussionmentioning

confidence: 99%

“…Another concern of our work was optimizing the processes in daily laboratory routine diagnostics. (Cieslak et al, 2017Dixon, Paley, Alegria-Moran, & Oidtmann, 2016;Enzmann et al, 2010;Enzmann, Kurath, Fichtner, & Bergmann, 2005;Haenen et al, 2016;Jia et al, 2014;Oidtmann, Pearce et al, 2014a;Oidtmann, Peeler et al, 2014b;Yoshimizu, 1996). Germany plays a central role in trade of rainbow trout because of its geographical position in Europe.…”

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confidence: 99%

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Improvement of a diagnostic procedure in surveillance of the listed fish diseases IHN and VHS

Hoferer

Akimkin

Skrypski

et al. 2019

Journal of Fish Diseases

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Infectious haematopoietic necrosis (IHN) and viral haemorrhagic septicaemia (VHS) are OIE‐listed and notifiable viral fish diseases which are controlled by eradication and surveillance programmes globally. The present study provides improved RT‐qPCR procedures based on recently described OIE protocols. Improvements comprise the design of a new TaqMan® probe, replacing a TaqMan® MGB probe that turned out to show impaired binding. Reason for this is SNPs detected in the nucleoprotein N gene sequences of IHNV strains targeted by the RT‐qPCR. Furthermore, the IHNV and VHSV RT‐qPCR assays were realized as one‐step and one‐run procedures supplemented by an endogenous control system. The IHNV and VHSV RT‐qPCR assays are characterized by a technical sensitivity of 19 and 190 gene equivalents (cRNA) and an analytical sensitivity of 2–7 and 13 TCID50/ml, respectively. For verification purposes, 105 IHNV and 165 VHSV isolates and several non‐targeted viral and bacterial pathogens were included and returned adequate results. However, in field samples divergent results left 14 samples of 154 undetected for IHNV and one sample of 127 for VHSV using cell culture. The study shows that RT‐qPCR assays ensure facilitated and reliable testing on IHNV and VHSV in eradication and surveillance programmes.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Improvement of a diagnostic procedure in surveillance of the listed fish diseases IHN and VHS

Hoferer

Akimkin

Skrypski

et al. 2019

Journal of Fish Diseases

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“…The world’s IHNV isolates have been divided into five genogroups, M, L, U, E, and J, based on the glycoprotein gene sequence. All Chinese IHNV isolates belong to genogroup J, together with the other Asian IHNV strains 35, 36 . Although there is only one IHN viral serotype 37 , mutations in the G gene can cause low virulence or produce neutralization-resistant variant IHNV strains 38 .…”

Section: Discussionmentioning

confidence: 99%

Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

Zhao

Liu

et al. 2017

Sci Rep

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Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are important pathogens of salmon and trout. An active bivalent DNA vaccine was constructed with the glycoprotein gene of Chinese IHNV isolate Sn1203 and VP2–VP3 gene of Chinese IPNV isolate ChRtm213. Rainbow trout (5 g) were vaccinated by intramuscular injection with 1.0 µg of the bivalent DNA vaccine and then challenged with an intraperitoneal injection of IHNV, IPNV, or both, at 30 and 60 days post-vaccination (d.p.v.). High protection rates against IHNV were observed, with 6% and 10% cumulative mortality, respectively, compared with 90–94% in the mock-vaccinated groups. IPNV loads (531-fold and 135-fold, respectively) were significantly reduced in the anterior kidneys of the vaccinated trout. Significant protection against co-infection with IHNV and IPNV was observed, with cumulative mortality rates of 6.67% and 3.33%, respectively, compared with 50.0% and 43.3%, respectively, in the mock-vaccinated groups. No detectable infective IHNV or IPNV was recovered from vaccinated trout co-infected with IHNV and IPNV. The bivalent DNA vaccine increased the expression of Mx-1 and IFN-γ at 4, 7, and 15 d.p.v, and IgM at 21 d.p.v., and induced high titres (≥160) of IHNV and IPNV neutralizing antibodies at 30 and 60 d.p.v.

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“…In regards to wild fish of Japan, the incidence of PRV‐2 in wild native salmon species remains unknown. In salmon and trout farming industry of China and Korea, viral diseases such as infectious haematopoietic necrosis and infectious pancreatic necrosis (Ji et al., 2017; Jia et al., 2014; Kim et al., 2007; Suebsing, Kim, Kim, Park, & Oh, 2011) have been documented; however, neither HSMI‐ nor EIBS‐like diseases have been reported from salmon of this region suggesting that if PRV is present it has not been associated with significant disease.…”

Section: Prevalencementioning

confidence: 99%

Piscine orthoreovirus: Biology and distribution in farmed and wild fish

Polinski

Vendramin

Cuenca

et al. 2020

Journal of Fish Diseases

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Piscine orthoreovirus (PRV) is a common and widely distributed virus of salmonids. Since its discovery in 2010, the virus has been detected in wild and farmed stocks from North America, South America, Europe and East Asia in both fresh and salt water environments. Phylogenetic analysis suggests three distinct genogroups of PRV with generally discrete host tropisms and/or regional patterns. PRV-1 is found mainly in Atlantic (Salmo salar), Chinook (Oncorhynchus tshawytscha) and Coho (Oncorhynchus kisutch) Salmon of Europe and the Americas; PRV-2 has only been detected in Coho Salmon of Japan; and PRV-3 has been reported primarily in Rainbow Trout (Oncorhynchus mykiss) in Europe. All three genotypes can establish high-load systemic infections by targeting red blood cells for principal replication. Each genotype has also demonstrated potential to cause circulatory disease. At the same time, high-load PRV infections occur in non-diseased salmon and trout, indicating a complexity for defining PRV's role in disease aetiology. Here, we summarize the current body of knowledge regarding PRV following 10 years of study.

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Determination of the complete genome sequence of infectious hematopoietic necrosis virus (IHNV) Ch20101008 and viral molecular evolution in China

Cited by 23 publications

References 22 publications

Improvement of a diagnostic procedure in surveillance of the listed fish diseases IHN and VHS

Improvement of a diagnostic procedure in surveillance of the listed fish diseases IHN and VHS

Bivalent DNA vaccine induces significant immune responses against infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus in rainbow trout

Piscine orthoreovirus: Biology and distribution in farmed and wild fish

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