Determination of the content and identity of lidocaine solutions with UV–visible spectroscopy and multivariate calibration

Abstract

A method is proposed for the determination of the content and identity of the active compound in pharmaceutical solutions by means of ultraviolet-visible (UV-Vis) spectroscopy, orthogonal signal correction (OSC) and multivariate calibration with soft independent modelling of class analogy (SIMCA) classification and partial least squares (PLS) regression. The content was determined with PLS regression and the identity with PLS regression and SIMCA classification. The method was tested on the local anaesthetic compound lidocaine. For the validation, external test sets of both manufactured sample solutions and samples from a stability study were used. For comparison with this new method, liquid chromatography was used as a reference method. The results show that in respect of accuracy, precision and repeatability, the new method is comparable to the reference method. The main advantage over liquid chromatography is the much shorter time of analysis and the simpler analytical procedure. An estimate of the analysis time saved with the proposed method compared with using liquid chromatography, together with practical considerations, is given.

Monitoring substrate and products in a bioprocess with FTIR spectroscopy coupled to artificial neural networks enhanced with a genetic-algorithm-based method for wavelength selection

“…On the other hand, chemometric applications to improve information obtained from modern instrumental data (i.e. spectral and electrochemical data) in different fields have acquired a routine character [12][13][14][15][16][17][18][19][20][21][22]. Partial least-squares (PLS) has become a usual tool for multivariate calibration because of the quality of the obtained calibration models, the ease of its implementation and the availability of software [23,24].…”

Preliminary Study for the Preparation of Transmucosal or Transdermal Patches with Acyclovir and Lidocaine

“…Figure 9 a,b show the absorbance obtained in the case of the membranes studied compared to the standard of the active substance. Av and L were visible in all membranes having the maximum absorbance for L at 266 nm and membranes with Av content at 288 nm [ 38 , 39 ]. Following the analysis by UV-Vis spectroscopy, the presence of the active substance in the synthesized membranes is observed in all cases.…”

Development of a Fast Simultaneous Analysis Method for Determination of Middle Rare-Earth Elements in Monazite Samples

“…They had an overlapping spectrum (shown in Figure 2), which informed that not possible determined by univariate analysis. Wiberg et al [ 27 ] reported that the uninformative shape of UV-vis absorption spectra was possible with multivariate data analysis to obtain good results for both quantitative and qualitative determinations.…”

“…Although inductively coupled plasmaoptical emission spectrometry (ICP-OES) is quite good in determining the REEs [ 24 , 25 , 26]; however, it is timeconsuming and expensive. On the other hand, modern automatic analysis methods provide opportunities to collect large amounts of data very easily and are considered valuable and promising ones due to their fast, robustness, cost-effectiveness [ 27 ]. Thus, developing an analysis method for the determination of the REEs based on the modern automatic analysis is desirable.…”