Determination of the Dexlansoprazole in Bulk and Spiked Human Plasma by Extraction Spectrophotometry

Abstract

ABSTRACT. This paper describes a simple extraction spectrophotometric method for the quantification of dexlansoprazole in bulk and spiked human plasma. This method involves formation of stable yellow colored chloroform extractable ion-pair complex of the amino derivative of dexlansoprazole with acid dye, namely methyl orange in acidic medium. The ion-pair complexes exhibit absorption maxima at 425 nm. Dexlansoprazole can be determined up to 4-40 μg/mL by the proposed method. The effect of optimum reagent concentration was studied. The relative standard deviations (≤1. 246%) obtained in the intra-day and inter-day analyses were found to be satisfactory. The accuracy results exhibited the mean recovery and percentage error in the range of 99.137%-100.574% and 0.012%-0.863%. When applied for the assay of the dexlansoprazole in spiked human plasma sample, recovery mean values ranged from 96.495-98.960%. The proposed method is useful for the estimation of the dexlansoprazole in bulk and human plasma samples.

Proton Pump Inhibitor Dexlansoprazole in Pure Form and Pharmaceutical Formulations Using Alizarin Derivatives

“…A through literature search has revealed that only few spectrophotometric methods available for determination of DXL in pure and dosage forms. 10,11 However, many of the above methods suffered from one or other disadvantage like poor sensitivity, require high cost solvents in addition to elaborate treatment, need tedious extraction procedures, measurements done at shorter wavelengths, heating or cooling step, use of expensive chemical and/or complicated experimental set-up. Spectrophotometric technique, because of simplicity and low cost, sensitivity and selectivity, significant accuracy and precision and broad availability and applicability for pharmaceutical analysis.…”