Determination of the enantiomers of salbutamol and its 4‐O‐sulphate metabolites in biological matrices by chiral liquid chromatography tandem mass spectrometry

Joyce, Karina B.; Jones, Anne E; Scott, Rebecca J.; Biddlecombe, Robert A.; Pleasance, Stephen

doi:10.1002/(sici)1097-0231(19981215)12:23<1899::aid-rcm417>3.3.co;2-9

Cited by 22 publications

(33 citation statements)

References 7 publications

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“…The plasma levels of d-threo-MPH were substantially higher than those of the l-threo-MPH, which is in accord with previous findings. 1 Recently, Joyce et al 29 reported the determination of salbutamol enantiomers and its 4-O-metabolite using a teicoplanin-based CSP coupled to a triple quadrupole mass spectrometer. Teicoplanin (Chirobiotic T) is also a macrocyclic glycopeptide, which exhibits complementary stereoselectivity to the vancomycin CSP.…”

Section: Resultsmentioning

confidence: 99%

Liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry enantiomeric separation ofdl-threo-methylphenidate, (Ritalin®) using a macrocyclic antibiotic as the chiral selector

Ramos

Bakhtiar

Majumdar

et al. 1999

Rapid Commun. Mass Spectrom.

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Vancomycin, a macrocyclic antibiotic, is an amphoteric glycopeptide produced by Streptomyces orientalis which has proven to be a viable chiral selector for high performance liquid chromatograph (HPLC) (D. W. Armstrong, Y. Tang, S. Chen, Y. Zhou, C. Bagwill and J-R. Chen, Anal. Chem. (1994; 66: 1473). While it is related to other glycopeptide antibiotics, vancomycin has a number of unique structural features, including 18 stereogenic centers, five aromatic rings, and two side chains one of which is a carbohydrate dimer. Therefore, a vancomycin-based stationary phase appears to be multimodal in that it can be utilized in both normal-phase and reversed-phase liquid chromatography. Consequently, the enantiomeric separation may be operative via several mechanisms, including pi-pi complexation, dipole stacking, inclusion, hydrogen bonding, or combinations of these interactions. LC/MS/MS is a powerful tool for quantitative analysis when evaluated on the basis of speed, specificity, reliability and sensitivity. For these reasons, the present paper explored the feasibility of bonded macrocyclic glycopeptide phases for chiral LC/MS/MS quantitative analysis. Methylphenidate was used as a model compound. A rapid chiral bioanalytical method (<7.5 min) for the determination of the enantiomers of methylphenidate was developed. A lower limit of quantification (LLOQ) of 87 pg/mL was attained for the human plasma assay. This is to our knowledge the first example of enantioselective reversed-phase LC/MS/MS for methylphenidate. The chiral column was relatively cost effective and exhibited excellent performance with no separation deterioration observed after approximately 2500 injections.

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Section: Resultsmentioning

confidence: 99%

Liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry enantiomeric separation ofdl-threo-methylphenidate, (Ritalin®) using a macrocyclic antibiotic as the chiral selector

Ramos

Bakhtiar

Majumdar

et al. 1999

Rapid Commun. Mass Spectrom.

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“…Serum salbutamol concentrations were measured with liquid chromatography-mass spectrometry by Glaxo Wellcome as reported previously. 18 Standard curves were generated using calibration standards at 0.1, 2, 4, 8, 16, 25, and 50 ng/ml salbutamol. For all standard concentrations, the inter-and intra-assay precision (%CV) and the bias were all less than 15% over the limits of quantification.…”

Section: Methodsmentioning

confidence: 99%

Two administration methods for inhaled salbutamol in intubated patients

Garner

Wiest²,

Bradley³

et al. 2002

Archives of Disease in Childhood

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Aims: To compare serum concentrations and effects on respiratory mechanics and haemodynamics of salbutamol administered by small volume nebuliser (SVN) and metered dose inhaler (MDI) plus spacer. Methods: Blinded, randomised, crossover study in 12 intubated infants and children (mean age 17.8 months) receiving inhaled salbutamol therapy. Subjects received salbutamol as 0.15 mg/kg by SVN and four puffs (400 µg) by MDI plus spacer at a four hour interval in random order. Passive respiratory mechanics were measured by a single breath/single occlusion technique, and serum salbutamol concentrations by liquid chromatography-mass spectrometry at 30 minutes, 1, 2, and 4 hours after each dose. Haemodynamics (heart rate and blood pressure) were recorded at each measurement time.Results: There was no difference in percentage change in respiratory mechanics or haemodynamics between the two methods of administration. Mean area under the curve (AUC 0-4 ) was 5.86 for MDI plus spacer versus 4.93 ng/ml × h for SVN. Conclusions: Serum concentrations and effects on respiratory mechanics and haemodynamics of salbutamol were comparable with the two administration methods under the conditions studied. Future studies are needed to determine the most effective and safe combination of dose and administration method of inhaled salbutamol in mechanically ventilated infants and children.

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“…But the sensitivity of the method with UV detector was not adequate to support bioanalytical studies. The advent of combined LC‐MS has revolutionized bioanalysis providing sensitive and specific methods with fast analysis time . A few LC‐MS/MS methods have been reported for the determination of trantinterol in biological matrices .…”

Section: Introductionmentioning

confidence: 99%

Determination of L‐trantinterol in rat plasma by using chiral liquid chromatography‐tandem mass spectrometry

Jing

Qin

et al. 2012

J of Separation Science

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A simple, sensitive, and rapid method for determination of L-trantinterol in rat plasma was developed for the first time by using LC coupled to MS/MS based on chiral stationary phase. A baseline separation of the enantiomers of trantinterol was achieved on a Chirobiotic V column, using a mixture of acetonitrile-methanol-ammonia-acetic acid (80:20:0.01:0.02, v/v/v/v) as the mobile phase. The detection was performed on a triple-quadrupole tandem mass spectrometer by multiple reaction monitoring mode via ESI. The calibration curve was linear in concentration range from 0.270 to 108 ng/mL in plasma with the lower limit of quantification of 0.270 ng/mL. The intra- and interday precision (relative standard deviation) values were within 10.9% and the accuracy (relative error) was from 2.6 to 9.2% at all quality control levels. The method has been successfully applied to a study of L-trantinterol pharmacokinetics in rats.

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Determination of the enantiomers of salbutamol and its 4‐O‐sulphate metabolites in biological matrices by chiral liquid chromatography tandem mass spectrometry

Cited by 22 publications

References 7 publications

Liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry enantiomeric separation ofdl-threo-methylphenidate, (Ritalin®) using a macrocyclic antibiotic as the chiral selector

Liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry enantiomeric separation ofdl-threo-methylphenidate, (Ritalin®) using a macrocyclic antibiotic as the chiral selector

Two administration methods for inhaled salbutamol in intubated patients

Determination of L‐trantinterol in rat plasma by using chiral liquid chromatography‐tandem mass spectrometry

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