Determination of the Infective Strain of Hydatid Cyst in Iraqi Cattle by Using Co1 Gene

Muhaidi, Mohammed J.

doi:10.36103/ijas.v48i2.432

Cited by 3 publications

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“…These investigations found the G1, G3, and G7 genotypes in humans and G1 and G3 genotypes in sheep and cattle. The most common genotypes in Iraq are G1 and G3, affecting humans, sheep, and cattle [ 30 ]. Al-Asadi et al [ 31 ] addressed a human infection with the G1 genotype, whereas Fadhil and A’aiz [ 32 ] investigated animal infections with the genotypes (G1-G3).…”

Section: Discussionmentioning

confidence: 99%

G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq

Al-Ardi

2024

J Vet Sci

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Background Infections of cats with Echinococcus granulosus is uncommon because the cat is not part of the parasite life cycle that a carnivorous and another herbivore represent. Nevertheless, it occurs incidentally when eating food or drinking water contaminated with the worm’s larva, especially with the presence of the definitive host (dogs), in this case, the infections are concentrated in stray or outside cats. For this reason, this study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection. Objective This study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection. Methods Four of the 37 cats that had died in different accidents developed cystic echinococcosis (CE). The cytochrome c oxidase subunit I (COX1) gene was initially amplified and sequenced to determine if these cysts belonged to E. granulosus, in beginning. The DNA fragments resulting from sequencing were then compared and aligned with other sequences using the Gene Bank database. Finally, a phylogenetic tree was drawn according to the sequence data obtained from cox1 genes sequencing, and the MEGA 7.0 phylogenetic analysis program was utilized. Results Four different sequences were deposited in the Gen Bank with accession numbers (ON795961 to ON795964), all of which belong to the G1 genotype. Approximately 84% and 100% of these sequences aligned with G1 (AB622277.1) and G1 (MG722980.1), respectively. Conclusions G1 is the dominant genotype that causes cat infections, even though the cat’s EC infection was incidental.

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Section: Discussionmentioning

confidence: 99%

G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq

Al-Ardi

2024

J Vet Sci

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“…These results are in accordance with previous studies. 15,18,24,26,27 It is the main reason of being such strain as more frequent and spread in the intermediate hosts for these reasons. 16,28 E. granolosus strain detection in domestic animals as well as in wild animal in this region will be epidemiologically significant.…”

Section: Discussionmentioning

confidence: 99%

Molecular Study Of Hydatid Disease In Iraqi Goats By using The Mitochondrial Cytochrome C Oxidase Subunit 1 Gene

Muhaidi¹

2022

J. Pure Appl. Microbiol.

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The aim of the present study was to identify and recognize the genotype for cystic Echinococcosis that infects goats (n=19) in Iraq. The hydatid cyst was collected from different parts of the body, i.e. lungs, liver, heart, spleen and peritoneal cavity. The current study was conducted in five different regions of Iraq (Anbar, Baghdad, Saladdin, Karkuk, Babylon during October 2018 to July 2019. The mitochondria DNA was extracted and screened for the presence of (CO1) cytochrome C oxidase subunit 1 gene using polymer chain reactions (PCR). Amplification size was around 450bp. The amplicon was isolated and purified. The isolated CO1 amplicon was sequenced. The CO1 putative sequence was BLAST with available sequence from NCBI. The genetic tree was deduced. The genotype G1 is the most frequently spread strains and considered as a key source for infection in goats of Iraq.

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Morphological and Bioinformatics Study for Radix Auricularia Snails in Freshwater in Basrah Province, Iraq

AL-Asadi¹

2021

Iraqi J. Agric. Sci.

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Radix auricularia (Lymnaea auricularia) snails are the main intermediate host of Fasciola gigantica in the middle and South of Iraq. Recently, some studies suggest these snails are not present in Iraq, in particular Basrah province. Thus, the current study was aimed to use the bioinformatics analysis in the determination of Radix sp. in Basrah and also aimed to identify morphological characteristics of this species in the AL-Sewaib river, Basrah. Four hundred R. auricularia snails were collected from the AL-Sewaib river during May 2018 to March 2019. The results of the bioinformatics analysis showed according to the mitochondrial cytochrome c oxidase subunit I, the presence of R. auricularia snails in Basrah province and these snails are more closely related to Iranian R. auricularia snails than those for European counties and Russia. This study also displayed the existence of the polymorphism phenomenon in R. auricularia snails collected from the AL-Sewaib river which there were six different forms (A, B, C, D, E and F). These forms had variations in the colour and form of shells along with a variation in the number of eggs (30-56) per an egg mass. In addition to this, these R. auricularia forms had variations in their distributions. Form E had the highest spread percentage (33%) followed by 17, 15, 14, 11 and 10% for forms B, D, C, A and F, respectively. These forms had also variations in their morphological characteristics. The largest form was the form D whereas the smallest form was the form B but other forms A, C, E and F were similar and had moderate sizes.Thus, the current study concludes the existence of R. auricularaia snails in Basrah as a representative city for the southern part of Iraq and also confirmed the presence of the polymorphism phenomenon in the members of R. auricularaia obtained from the AL-Sewaib river.

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Determination of the Infective Strain of Hydatid Cyst in Iraqi Cattle by Using Co1 Gene

Cited by 3 publications

References 0 publications

G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq

G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq

Molecular Study Of Hydatid Disease In Iraqi Goats By using The Mitochondrial Cytochrome C Oxidase Subunit 1 Gene

Morphological and Bioinformatics Study for Radix Auricularia Snails in Freshwater in Basrah Province, Iraq

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