Determination of the length of sedimentation reaction in blood using the TEST 1 system: comparison with the Sedimatic 100 method, turbidimetric fibrinogen levels, and the influence of M-proteins

Ajubi, N.E.; Bakker, A.; Berg, G.

doi:10.1515/cclm.2006.151

Cited by 12 publications

(13 citation statements)

References 9 publications

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“…Erythrocyte sedimentation rate is elevated in various infectious diseases, infarctions, malignancies, rheumatic disorders, autoimmune diseases, and mono‐ or polyclonal gammopathies . The common denominator is the increased production of plasma (monoclonal) proteins that stimulate RBC aggregation which, in turn, increase the ESR.…”

Section: Discussionmentioning

confidence: 99%

“…ESR refers to the distance (not the rate) that the red blood cells (RBCs) settle toward the bottom of a vertical tube of well‐mixed venous blood during a given time interval (normally 1 hour); it is expressed in mm/h . The ESR increases during inflammation owing to the accelerated sedimentation of RBCs following an increase in the plasma concentration of acute phase proteins of large molecular size . The Westergren method for measuring ESR is recommended by the International Council for Standardization in Haematology (ICSH), and modified Westergren method using EDTA‐anticoagulated blood is known to correlate well with the Westergren method …”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11][12] The ESR increases during inflammation owing to the accelerated sedimentation of RBCs following an increase in the plasma concentration of acute phase proteins of large molecular size. [1][2][3][4][5][6][7][8][9][10][11][12][13] The Westergren method for measuring ESR is recommended by the International Council for Standardization in Haematology (ICSH), and modified Westergren method using EDTA-anticoagulated blood is known to correlate well with the Westergren method. 8,9 The measurement of ESR consists of 3 stages of sedimentation reactions: the red cell rouleaux formation in which erythrocytes aggregate for the first 10 minutes; sedimentation, which occurs over the next 40 minutes; and cell packing, which occurs in the last 10 minutes.…”

Section: Introductionmentioning

confidence: 99%

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Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases

Kim

Lee

et al. 2018

Int J Lab Hematology

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases

Kim

Lee

et al. 2018

Int J Lab Hematology

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“…In most evaluations, new analyser methods were compared with the standardized method with diluted samples (Thomas & Karpic, 1993; Happe et al. , 2002; Romero, Munoz & Ramirez, 2003; Al‐Fadhli & Al‐Awadhi, 2005; Ajubi, Bakker & van den Berg, 2006; Mahlangu & Davids, 2008; Shelat, Chacosky & Shibutani, 2008; Alexy, Pais & Meiselman, 2009; Hardeman et al. , 2010a,b; Perovic, Bakovic & Valcic, 2010).…”

Section: Review Of the Literature: Use Of The Esr Reference And Standmentioning

confidence: 99%

ICSH review of the measurement of the erythocyte sedimentation rate

Jou¹,

Lewis²,

Briggs³

et al. 2011

International Journal of Laboratory Hematology

120

130

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In recognition of the need for a standardization of the measurement of the erythrocyte sedimentation rate (ESR), the International Council for Standardization in Haematology makes the following recommendations: (i) The reference method for measurement of the ESR should be based on the Westergren method, which is a specific test for the ESR, with modifications, (ii) The reference method for measurement of the ESR should use either whole blood anticoagulated with EDTA and later diluted with sodium citrate or saline (4 : 1) or whole blood anticoagulated with sodium citrate (4 : 1) in Westergren pipettes, (iii) The ESR pipettes can be of glass or plastic (with specific characteristics). It must be colourless; a minimum sedimentation scale of 200 mm, a minimum bore of 2.55 mm, which should be constant within 5%. A protocol for the evaluation of alternative methodologies against the reference method is outlined: The new technologies must be tested over a range of ESR values of 2-120 mm. In this comparison, 95% of the differences should be 5 mm or less, with larger differences associated with higher ESR values. A minimum of 40 samples should be tested in 3 different groups of values: 1-20, 21-60 and more than 60 mm. The statistical methods recommended for ESR evaluations are the coefficient of correlation, the Passing-Bablock regression and the Bland-Altman statistical method. This reference method replaces all earlier standardized and reference methods.

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“…Some instruments require anticoagulated blood with sodium citrate, whereas the most recent ones measure ESR from EDTA undiluted samples (reference specimen) (11). The EDTA-anticoa gu lated sample has several advantages: (i) it preserves the red blood cell morphology, (ii) it does not interfere with mechanisms that lead to erythrocyte sedimen tation, (iii) it increases specimen stability, and (iv) it does not incur problems related to sample dilution with sodium citrate, in view of the fact that the ratio between blood and anticoagulant is of great importance and failure to respect the standard may explain specimen rejection in routine practice.…”

Section: The »New« Esr Testmentioning

confidence: 99%

The Esr Test: An Old Test With New Contents

Piva¹,

Plebani²

2010

Journal of Medical Biochemistry

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Kratak sadr`aj: Brzina sedimentacije eritrocita (ESR) osta je jedan od naj~e{}e kori{}enih laboratorijskih testova. Klini~ku primenu i korisnost ovaj test ima u pra}enju inflamatornih bolesti, naro~ito reumatoidnog artritisa, temporalnog arteritisa i reumatske polimijalgije. Referentni metod za merenje ESR predlo`en od strane Me|unarodnog komiteta za standardizaciju u hematologiju koristi punu krv sa antikoagulansom EDTA za izvo|enje testa pomo}u metode koju je 1921. opisao Westergren. Trenutno zanima nje za metodologiju fokusirano je na razvoj automatizovanih za tvorenih sistema koji omogu}avaju odre|ivanje brzine sedimentacije uz odabrane radne metode, koje koriste jedan uzorak za vi{e hematolo{kih testova i unapre|uju biohazardne aspekte postupka testiranja. Usled toga, standardizacija postaje neophodna. Rezultati ESR moraju biti po uzdani uprkos pove}anom broju razli~itih metoda i varijabli za testiranje. Danas su dostupni kontrolni materijali i {eme za osiguranje spolja{njeg kvaliteta i treba ih koristiti. Dakle, inovativne tehnike mogu dalje opravdati korisnost ESR u klini~koj praksi, ali pored toga moraju garantovati sledivost rezultata u pore|enju sa referentnom metodom kako bi se obezbedila uporedivost rezultata izme|u vi{e klini~kih laboratorija.Klju~ne re~i: brzina sedimentacije eritrocita, referentne i odabrane procedure, kontrola kvaliteta, {eme za osiguranje spolja{njeg kvaliteta The »old« ESR testIn 1897, the Polish pathologist Biernacki first announced that »the rate at which red corpuscles separated could help identify a patient's disease and that the increased sedimentation rate of blood from ill individuals was due to the presence of fibrinogen« (1). Later, in 1918 the Swedish pathologist and haematologist Robert (Robin) Sanno Fåhraeus furthered Biernacki's work, initially using the erythrocyte sedimentation rate (ESR) as a pregnancy test. Along with the first descriptions by Fåhraeus, Alf Vilhelm Albertsson Westergren, a Swedish internist, developed the test method that is named after him (2-3).Since the 19th century, the erythrocyte sedimen tation rate has become one of the most common Summary: The erythrocyte sedimentation rate (ESR) remains one of the most widely used laboratory tests. Its clinical usefulness and interpretation are in the monitoring of inflammatory diseases, in particular rheumatoid arthritis, temporal arteritis and polymyalgia rheumatica. At present, the reference method for measuring the ESR proposed by the International Committee for Standardization in Haematology (ICSH) utilizes EDTA-anticoagulated-undiluted blood to perform the test using the method described by Westergren in 1921. Current interest in the methodology focuses on the development of an automated closed system that allows the determination of the sedimentation rate with selected working methods, using a single sample for more than one haematological test, improving the bio-hazardous aspects of the testing procedures. As a consequence, standardization becomes necessary. ESR results should be reliable, desp...

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Determination of the length of sedimentation reaction in blood using the TEST 1 system: comparison with the Sedimatic 100 method, turbidimetric fibrinogen levels, and the influence of M-proteins

Abstract: The results confirm previous reports that TEST 1 is a reliable method to measure the LSRB, and shows for the first time the quantitative relationship between TEST 1 LSRB and M-proteins, particularly those of the IgM type.

Cited by 12 publications

References 9 publications

Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases

Erythrocyte sedimentation rate measured using microhemagglutination is not elevated in monoclonal gammopathy compared with other diseases

ICSH review of the measurement of the erythocyte sedimentation rate

The Esr Test: An Old Test With New Contents

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