Determination of the Main Forces Driving DNA Oligonucleotide Adsorption onto Aminated Silica Wafers

Balladur, Véronique; Théretz, Alain; Mandrand, Bernard

doi:10.1006/jcis.1997.5123

Cited by 85 publications

(85 citation statements)

References 35 publications

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“…21, where an increasing pH increases or decreases film thickness, in dependence of the polyelectrolyte system chosen. In fact, most studies on pH effects report an optimum pH range 11,32,129,187,190,208,230,261,276,322,394,399,425) . Extreme pH values may completely prevent film growth by favoring desorption 32,71,130,253,322,414) .…”

Section: Experimental Conditions and Parametersmentioning

confidence: 98%

“…However, some very fast adsorbing systems are known 211,243,266,312,314,316,321,322,424) , as well as some slow cases 96,102,135,139,149,229,279,365,386,425) . In any case, molar mass effects, pH and ionic strength may influence the kinetics, and adsorption kinetics seem to be transport-limited only in the very beginning of the process 129) .…”

Section: Experimental Conditions and Parametersmentioning

confidence: 99%

See 1 more Smart Citation

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,175

1,184

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A bit of historyThe key role of surfaces for many material properties as well as for many biological processes has been recognized now. Therefore, new strategies aim at the tailoring of the material's surface only -or of a thin surface layer, respectively -, while preserving the bulk properties of the underlying support. Particular emphasis has been given to the surface modification by polymers, in an attempt to extend the known versatility of polymer bulk materials to ultrathin films and coatings, and to prepare bulk-surface composite materials. The self-organization of polymers has been increasingly explored for the preparation of well-defined surfaces and interfaces in recent years, extending the use of the established methods of low molar mass compounds (for comparative reviews, see ref. 1-3)). With such techniques, polymer films are formed spontaneously on substrates, due to balanced interactions between substrate, polymer (or its precursor) and medium. Typically, very thin, often monomolecular layers are produced. Repetitive deposition steps provide a precise control over the total thickness of the coatings, in the range from a few angstroms up to the micrometer range. Moreover, the step-by-step procedures allow for a fine structuring in the third dimension. In addition to the preparation of uniform and homogeneous coatings, gratings, gradients or steps of defined height in molecular dimensions can be easily constructed.The most recent of the self-organization techniques is the alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA) [3][4][5][6][7][8][9][10] . Although some early, singular studies of self-assembly by alternating adsorption of oppositely charged polyions are reported [11][12][13] , a practical method for ESA was developed Feature Article: The article presents the state-of-the-art of alternating physisorption of oppositely charged polyelectrolytes, the so-called "layer-by-layer" method or "electrostatic self-assembly" (ESA), for the preparation of thin polymer coatings. In comparison to other, more established self-organization techniques, this recent method is distinguished by its simplicity, versatility, and speed. In particular, the tendency for self-healing is unique. Emphasis is given to the role of the molecular structure of the polyelectrolytes, and to the nature of the support. Also, various parameters for the preparation of multilayer films are highlighted, which are very important due to the kinetic control of the build-up process. The structure of the resulting coatings, their quality and stability, chemical reactions in the films, and potential applications are discussed.Macromol. Rapid Commun. 21, No. 7

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Section: Experimental Conditions and Parametersmentioning

confidence: 98%

Section: Experimental Conditions and Parametersmentioning

confidence: 99%

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Bertrand

Jonas

Laschewsky³

et al. 2000

Macromol. Rapid Commun.

1,175

1,184

View full text Add to dashboard Cite

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“…In order to evaluate the electrostatic effect of silanols on wetting, the respective charges were divided according to the scheme (see Figure 4): (10) where q total is the total charge of each pair of silanol groups and its neighboring oxygens, q neigh_O is the half of the charge corresponding to six oxygens, and q viccinal is the charge of each pair of viccinal silanols. The initial charges for the silanol hydroxyl group were taken from the serine hydroxyl group of the CHARMM force field.…”

Section: Silica-water Systemsmentioning

confidence: 99%

Water−Silica Force Field for Simulating Nanodevices

2006

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Amorphous silica is an inorganic material that is central for many nanotechnology appplications, such as nanoelectronics, microfluidics, and nanopore technology. In order to use molecular dynamics (MD) simulations to study the behavior of biomolecules with silica, we developed a force field for amorphous silica surfaces based on their macroscopic wetting properties that is compatible with the CHARMM force field and TIP3P water model. The contact angle of a water droplet with silica served as a criterion to tune the intermolecular interactions. The resulting force field was used to study the permeation of water through silica nanopores, illustrating the influence of the surface topography and the intermolecular parameters on permeation kinetics. We find that minute modeling of the amorphous surface is critical for MD studies, since the particular arrangement of surface atoms controls sensitively electrostatic interactions between silica and water.

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“…This effect may be the result of electrostatic attraction between the positively charged amines of AEAPS and the negatively charged phosphodiester groups of DNA, as suggested for oligonucleotide adsorption on aminated silica. 48 The low DNA thickness also can be the result of spatial non-uniformities. In AFM studies of DNA covalently attached to thiol-terminated silane on silicon, oligonucleotide clusters of size 250 nm 2 were reported on the surface.…”

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confidence: 98%

Spectroscopic quantification of covalently immobilized oligonucleotides

Saprigin

Thomas

Dulcey

et al. 2005

Surf. Interface Anal.

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Quantitative determination of surface coverage, film thickness and molecular orientation of DNA oligomers covalently attached to aminosilane self-assembled monolayers has been obtained using complementary infrared and photoelectron studies. Spectral variations between surface immobilized oligomers of the different nucleic acids are reported for the first time. Carbodiimide condensation was used for covalent attachment of phosphorylated oligonucleotides to silanized aluminum substrates. Fourier transform infrared (FTIR) spectroscopy and x-ray photoelectron spectroscopy (XPS) were used to characterize the surfaces after each modification step. Infrared reflection-absorption spectroscopy of covalently bound DNA provides orientational information. Surface density and layer thickness are extracted from XPS data. The surface density of immobilized DNA, 2-3 (×10 13 ) molecules cm −2 , was found to depend on base composition. Comparison of antisymmetric to symmetric phosphate stretching band intensities in reflection-absorption spectra of immobilized DNA and transmission FTIR spectra of DNA in KBr pellet indicates that the sugar-phosphate backbone is predominantly oriented with the sugar-phosphate backbone lying parallel to the surface, in agreement with the 10-20Å DNA film thickness derived from XPS intensities.

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Determination of the Main Forces Driving DNA Oligonucleotide Adsorption onto Aminated Silica Wafers

Cited by 85 publications

References 35 publications

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Ultrathin polymer coatings by complexation of polyelectrolytes at interfaces: suitable materials, structure and properties

Water−Silica Force Field for Simulating Nanodevices

Spectroscopic quantification of covalently immobilized oligonucleotides

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