Determination of the ubiquinol-10 and ubiquinone-10 (coenzyme Q10) in human serum by liquid chromatography tandem mass spectrometry to evaluate the oxidative stress

Ruiz‐Jiménez, José; Priego-Capote, Feliciano; Mata-Granados, J.M.; Quesada, J. M.; Castro, M.D. Luque de

doi:10.1016/j.chroma.2007.10.055

Cited by 67 publications

(46 citation statements)

References 33 publications

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“…In previously reported LC-MS-based assays for CoQ10 in biological samples, either CoQ11 (14 ), CoQ9 (15)(16)(17)19 ), or dipropoxy-CoQ10 (18 ) has been used as the IS. A potential source of error for MS assays is ionization suppression that varies during a chromatographic run and between the samples.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial Coenzyme Q10 Determination by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry

2013

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BACKGROUND Coenzyme Q10 (CoQ10) is an essential part of the mitochondrial respiratory chain. Unlike most other respiratory chain disorders, CoQ10 deficiency is potentially treatable. We aimed to develop and validate an accurate liquid chromatography–tandem mass spectrometry (LC-MS/MS) method for the determination of mitochondrial CoQ10 in clinical samples. METHODS We used mitochondria isolated from muscle biopsies of patients (n = 166) suspected to have oxidative phosphorylation deficiency. We also used fibroblast mitochondria from 1 patient with CoQ10 deficiency and 3 healthy individuals. Samples were spiked with nonphysiologic CoQ10-[2H6] internal standard, extracted with 1-propanol and with ethanol and hexane (2 mL/5 mL), and CoQ10 quantified by LC-MS/MS. The method and sample stability were validated. A reference interval was established from the patient data. RESULTS The method had a limit of quantification of 0.5 nmol/L. The assay range was 0.5–1000 nmol/L and the CVs were 7.5%–8.2%. CoQ10 was stable in concentrated mitochondrial suspensions. In isolated mitochondria, the mean ratio of CoQ10 to citrate synthase (CS) activity (CoQ10/CS) was 1.7 nmol/U (95% CI, 1.6–1.7 nmol/U). We suggest a CoQ10/CS reference interval of 1.1–2.8 nmol/U for both sexes and all ages. The CoQ10/CS ratio was 5-fold decreased in fibroblast mitochondria from a patient with known CoQ10 deficiency due to recessive prenyl (decaprenyl) diphosphate synthase, subunit 2 (PDSS2) mutations. CONCLUSIONS Normalization of mitochondrial CoQ10 concentration against citrate synthase activity is likely to reflect most accurately the CoQ10 content available for the respiratory chain. Our assay and the established reference range should facilitate the diagnosis of respiratory chain disorders and treatment of patients with CoQ10 deficiency.

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Section: Discussionmentioning

confidence: 99%

Mitochondrial Coenzyme Q10 Determination by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry

2013

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“…Eluates were monitored by photodiode-array detector at three wavelengths, as we have previously described (Quesada et al, 2004). Coenzyme Q10 (CoQ10A or Q10, also known as ubiquinone-10) was determined by liquid chromatography coupled with tandem mass-spectrometry (LC-MS/MS), with electrospray ionization, as we have reported (Ruiz-Jiménez et al, 2007).…”

Section: Postmenopausal-women Treatment and Serum Analysesmentioning

confidence: 99%

Serum from postmenopausal women treated with a by-product of olive-oil extraction process stimulates osteoblastogenesis and inhibits adipogenesis in human mesenchymal stem-cells (MSC)

Casado-Díaz

Túnez‐Fiñana

Mata-Granados

et al. 2017

Experimental Gerontology

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Aging may enhance both oxidative stress and bone-marrow mesenchymal stemcell (MSC) differentiation into adipocytes. That reduces osteoblastogenesis, thus favoring bone-mass loss and fracture, representing an important worldwide health-issue, mainly in countries with aging populations. Intake of antioxidant products may help to retain bone-mass density. Interestingly, a novel olivepomace physical treatment to generate olive oil also yields by-products rich in functional antioxidants. Thus, diet of postmenopausal women was supplemented for two months with one of such by-products (distillate 6; D6), being rich in squalene. After treatment, serum from such women showed reduced both lipidic peroxidation and oxidized low-density lipoprotein (LDL). Besides, vitamin E and coenzyme Q10 levels increased. Furthermore, culture medium containing 10% of such serum both increased osteoblastogenesis and reduced adipogenesis in human MSC from bone marrow. Therefore, highly antioxidant by-products like D6 may represent a relevant source for development of functional products, for both prevention and treatment of degenerative pathologies associated with aging, like osteoporosis.

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“…The sensitivity of the proposed HPLC-CL method was approximately 60 times and 10 times higher than those of HPLC-UV [8] and HPLC with post-column colorimetric reaction method [9], respectively. Additionally, the sensitivity was almost comparable to those of HPLC-ECD with catalytic reduction [10], HPLC-ECD with electroreduction [11], HPLC-MS/MS [12] and ultra-performance liquid chromatography with mass spectrometry (UPLC-MS) [17] in spite of the complexity of the proposed detection system was lower compared to those of these methods.…”

Section: Calibration Curve and Detection Limitmentioning

confidence: 99%

“…Several analytical methods have been developed for the determination of ubiquinone in blood samples using high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection [8,9], electrochemical detection (ECD) [10,11] and tandem mass spectrometry (MS/MS) [12]. However, HPLC-UV methods lack sensitivity and suffer from interferences due to co-existing substances.…”

Section: Introductionmentioning

confidence: 99%

Selective determination of ubiquinone in human plasma by HPLC with chemiluminescence reaction based on the redox cycle of quinone

et al. 2011

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Ubiquinone is an important biologically active compound in living body. The determination of ubiquinone in human plasma is useful for the investigation of bioavailability of ubiquinone and for early diagnosis of several diseases. Therefore, we developed an HPLC-chemiluminescence detection method for the analysis of ubiquinone in plasma samples. The method is based on luminol chemiluminescence detection of super oxide anion that generated by the redox cycle reaction between ubiquinone and dithiothreitol. The HPLC system involved an octyl column with a mobile phase of methanol. Ubiquinone eluted from the column was mixed with dithiothreitol and luminol solutions simultaneously and generated chemiluminescence was monitored by chemiluminescence detector. The calibration curve for standard ubiquinone solution was linear from 0.09 to 43.2 µg/mL (0.22-216 ng on column) with the correlation coefficient of 0.999, and the detection limit (S/N = 3) was 26 ng/mL (130 pg on column). Using the proposed HPLC method, the peak of ubiquinone in human plasma could be clearly detected on the chromatogram without any interferences from plasma components.

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Determination of the ubiquinol-10 and ubiquinone-10 (coenzyme Q10) in human serum by liquid chromatography tandem mass spectrometry to evaluate the oxidative stress

Cited by 67 publications

References 33 publications

Mitochondrial Coenzyme Q10 Determination by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry

Mitochondrial Coenzyme Q10 Determination by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry

Serum from postmenopausal women treated with a by-product of olive-oil extraction process stimulates osteoblastogenesis and inhibits adipogenesis in human mesenchymal stem-cells (MSC)

Selective determination of ubiquinone in human plasma by HPLC with chemiluminescence reaction based on the redox cycle of quinone

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