Determination of Theophylline in Saliva, Using Fluorescence Polarization Immunoassay (FPIA)

Niemann, A.; Oellerich, Michael; Schumann, G.; Gw, Sybrecht

doi:10.1515/cclm.1985.23.11.725

Cited by 5 publications

(5 citation statements)

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“…However theophylline also stimulates the central nervous system (Kumer, 2004). Several studies have shown improvement of performance in racehorses 644 F. Beaudry et al ORIGINAL RESEARCH and fluorescence polarization immunoassay (Niemann, et al, 1985). All these methods lack precision and accuracy and are mainly used for screening purposes.…”

Section: Introductionmentioning

confidence: 99%

Development of an electrospray ionization mass spectrometric method for the quantification of theophylline in horse serum

Beaudry

Lavoie

Vachon

2005

Biomed. Chromatogr.

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A rapid and selective method has been developed for the determination of theophylline in horse serum by LC-ESI/MS/MS. The analytical method includes a protein precipitation extraction for sample preparation, liquid chromatography separation technique and ionspray tandem mass spectrometry. The drug was extracted from serum using a protein precipitation with acetonitrile and the supernatants were analyzed using an LC-ESI/MS/MS instrument. The chromatography was performed using a 50 x 2.1 mm C(8) analytical column and an isocratic mobile phase composes of 60:40 acetonitrile-0.5% formic acid in water with a flow rate fixed at 350 microL/min. A linear (weighted 1/concentration) relationship was used to perform the calibration over an analytical range of 0.1-20 ppm. The intra-batch precision and accuracy at LLOQ, medium and high concentration were 11.7, 6.9 and 5.4% and 95.8, 107.8 and 95.8%, respectively, and the inter-batch precision and accuracy at LLOQ, medium and high concentration were 10.4, 7.9 and 7.3% and 97.3, 105.2 and 95.9%, respectively. This LC-ESI/MS/MS method for the determination of theophylline in horse serum has been proved to within generally accepted criteria used for bioanalytical assay and was used successfully during clinical investigation.

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Section: Introductionmentioning

confidence: 99%

Development of an electrospray ionization mass spectrometric method for the quantification of theophylline in horse serum

Beaudry

Lavoie

Vachon

2005

Biomed. Chromatogr.

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“…The lower limit of the measured range was 0.5 mg/1. With this immunoassay the relative standard deviation between days was 3.6% over the mid-part of the measuring range (13,14). The performance of the FPIA was constantly checked by internal and external quality control.…”

Section: Determination Of Serum Theophylline Concentrationsmentioning

confidence: 92%

Evaluation of a Novel Bayesian Method for Individualizing Theophylline Dosage

Stein

Oellerich²,

Gw³

et al. 1988

Clinical Chemistry and Laboratory Medicine

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Summary:A novel Bayesian drug dosing program (Abbott Pharmacokinetic Systems, Theophylline Program) was evaluated. The predictive accuracy of this method was assessed in 10 healthy volunteers receiving single intravenous test doses. Estimates for clearance and distribution volume were compared with those obtained from the area under the curve. The observed prediction error depended largely on sampling time. The deviations were lowest for the distribution volume during the first 60 min and for clearance at 12 hours after theophylline administration. Furthermore the Bayesian technique was prospectively evaluated in 10 hospitalized and 22 outpatients treated with sustained-release theophylline preparations (Uniphyllin, Bronchoretard, PulmiDur). Predictive precision and accuracy were adequate, if theophylline was given twice daily. The highest predictive accuracy was achieved in outpatients, if predictions were based on trough concentrations. In 19/22 outpatients prediction errors were within a clinically acceptable range (mean prediction error + standard deviation; -0.6 ± 2.1 mg/1). Moreover in hospitalized patients (n = 5) with twice-daily maintenance regimens, concentration-time curves could mainly be predicted with sufficient accuracy. Hospitalized patients (n = 5) with once-daily dosing showed large fluctuations between peak and trough theophylline concentrations in serum. In these patients a reliable prediction of the concentration-time curves was not possible apparently due to non-linearity of theophylline kinetics. Relatively large prediction errors were found in one patient with acute viral respiratory illness and 3 patients with altered absorption. Despite certain limitations the clinical application of the Bayesian forecasting method tested appears to be promising.

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“…So far, the existence of this phenomenon has been shown for 2 examples: ethanol (56) and caffeine (57). However, they have interpreted their results by back diffusion from the oral mucosa (60,61). Theophylline is another candidate (40).…”

Section: Phenomenon Of Varying Arteriovenous Differencesmentioning

confidence: 99%

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1996

CCLM

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The determination of salivary drug concentrations is one of the major domains for the application of saliva in laboratory medicine. Its usefulness, however, has been criticized for many drugs because of the variability of the saliva/plasma concentration ratio (saliva/plasma ratio).Considering saliva as a model for transmembrane transport, drugs can be divided into 4 groups. The first group is characterized by a saliva/plasma ratio less than 1.0. In extreme cases a drug with negligible transport is either not detectable in saliva or is found in very low concentrations which are hard to measure or difficult to interpret.A second group leads to saliva/plasma ratios which are approximately constant and about 1.0 under most of the conditions studied. This group is ideal for monitoring salivary drug concentrations.A third group is sufficiently transferred into saliva, but the saliva/plasma ratio varies under different conditions. The reason for this variation is that the transport is influenced by several factors including an active transport mechanism. The varying influence of these factors on the saliva/plasma ratio largely depends on the physicochemical characteristics of the particular substance.In a fourth group very high saliva/plasma ratios are observed primarily due to the degree of ionization of weak bases.A representative of each group is presented with its saliva/plasma ratio and its physico-chemical properties: ceftazidim, ethanol, digoxin and prilocain. In all cases the salivary concentration probably reflects the intracellular concentration in target tissues. All examples confirm saliva as an ideal in vivo model for the study of transmembrane transport in the human organism.

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Determination of Theophylline in Saliva, Using Fluorescence Polarization Immunoassay (FPIA)

Cited by 5 publications

References 10 publications

Development of an electrospray ionization mass spectrometric method for the quantification of theophylline in horse serum

Development of an electrospray ionization mass spectrometric method for the quantification of theophylline in horse serum

Evaluation of a Novel Bayesian Method for Individualizing Theophylline Dosage

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