Determination of three main antileprosy drugs and their main metabolites in serum by high-performance liquid chromatography

Gidoh, Masaichi; Tsutsumi, Sadae; Takitani, Shoji

doi:10.1016/s0378-4347(00)80111-5

Cited by 20 publications

(1 citation statement)

References 15 publications

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“…Historically, clofazimine in human plasma or serum samples was analyzed by liquid chromatographic method with UV detection (Gidoh, Tsutsumi, & Takitani, ; Krishnan & Abraham, ; Peters, Hamme, & Gordon, ; Queiroz, Pereira, Gotardo, Cordeiro, & Melchior, ). A drawback of this method was the overall poor sensitivity and large sample volume.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of clofazimine (Lamprene®), an antileprosy agent, in human dried blood spots using liquid chromatography–tandem mass spectrometry

Doherty

et al. 2017

Biomedical Chromatography

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An LC-MS/MS method was developed and validated for bioanalysis of clofazimine in human dried blood spot (DBS) samples in support of a clinical study on multidrug-resistant tuberculosis in developing countries. The validated assay dynamic range was from 10.0 to 2000 ng/mL using a 1/8 inch DBS punch. The accuracy and precision of the assay were ±11.0% (bias) and ≤13.5% (CV) for the LLOQs (10.0 ng/mL) and ±15% (bias) and ≤15% (CV) for all other QC levels. The assay was proved to be free from the possible impact owing to spot size and storage temperature (e.g. at 60°C, ≤ - 60°C). The validated assay is well suited for the intended clinical study where conventional pharmacokinetic sample collection is not feasible.

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Section: Introductionmentioning

confidence: 99%