Determination of tianeptine in human plasma using high-performance liquid chromatography with fluorescence detection

Ulu, Sevgi Tatar

doi:10.1016/j.jchromb.2006.02.027

Cited by 20 publications

(1 citation statement)

References 11 publications

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“…For the routine analysis of BZDs and antidepressants various techniques have been used, such as: spectrophotometry and spectrofluorimetry [14,15], potentiometry [16], indirect kinetic catalytic method by stopped flow technique [17], nonaqueous CE [18], modified carbonpaste electrodes as sensors [19], immunoassay [20,21], radioreceptor assay [22], separation techniques like TLCdensitometry [23,24] and RPLC-ESI MS [25,26], Ultra Performance LC-MS/TOF (UPLC-MS/TOF) [27], micellar EKC (MEKC) [28,29], GC-nitrogen phosphorus detection (GC/ NPD) [30], GC-electron capture detection (GC-ECD) [31], GC-MS detection [32,33], GC-FID [34] and HPLC coupled with fluorescence detector [35], chemiluminescence detector [36], UV -Vis detector or HPLC-DAD [37,38], HPLC-ECD [39], HPLC-MS [40 -42], or HPLC-MS/MS [43].…”

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confidence: 99%

Development and validation of an HPLC method for the determination of benzodiazepines and tricyclic antidepressants in biological fluids after sequential SPE

Uddin

Samanidou

Papadoyannis³

2008

J of Separation Science

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A novel method for the simultaneous determination of six benzodiazepines (BZDs) and four tricyclic antidepressants (TCAs) in biological fluids by HPLC with UV detection at 240 nm has been developed. After a deproteinization step biological fluids were analyzed by direct injection. SPE on Nexus cartridges was also applied. Since two compounds, namely imipramine and diazepam, were coeluting, a sequential SPE protocol has been developed. BZDs were eluted by a mixture of methanol/ACN(1:1), followed by the elution of TCAs with methanol. Separation was performed on a Kromasil C8 column (250 x 64 mm(2) id, 5 microm) using a mobile phase of 0.05 MCH3COONH4/ACN/methanol (initial composition 55:15:30 v/v/v) at a flow rate of 1.0 mL/min delivered by a gradient program within 15 min. Colchicine was used as the internal standard (4 ng/microL). The method was linear for all analytes up to 20 ng/lL, with coefficients of regression between 0.996 and 0.99996. LODs and LOQs were 0.08-1.17 and 0.28-3.91 ng/lL, respectively. Recovery was in the range of 92.8-108.7% for within-day and 91.9-109.9% for between-day assays, with RSD values lower than 10.0% for all matrices.

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Section: Introductionmentioning

confidence: 99%

Development and validation of an HPLC method for the determination of benzodiazepines and tricyclic antidepressants in biological fluids after sequential SPE

Uddin

Samanidou

Papadoyannis³

2008

J of Separation Science

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Spectrofluorimetric and Spectrophotometric Determination of Oxamniquine in Pharmaceuticals and Biological Fluids via Derivatization with 4‐Chloro‐7‐Nitrobenzo‐2‐oxa‐1,3‐Diazole (NBD‐Cl)

El‐Enany

Ahmida

Belal

2009

J Chinese Chemical Soc

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Spectrophotometric and spectrofluorimetric methods were developed for the determination of oxamniquine (OXM). Both methods are based on coupling with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐Cl) in borate buffer of pH 7.6, and the reaction product was measured at 400 nm (Method I). The same product was measured by spectrofluorimetry at 480 nm upon excitation at 400 nm (Method II). The absorbance and the fluorescence intensity were enhanced by addition of sodium dodecyl sulphate (SDS). The absorbance‐concentration plot is rectilinear over the range of 5–25 μg/mL with an LOD of 0.31 μg/mL. The fluorescence‐concentration plot is linear over the range of 0.2–1.2 μg/mL with an LOD of 0.03 μg/mL. Both methods were applied to the analysis of capsules, and the results were in good agreement with those obtained using the official method. The method was applied to spiked human plasma; the mean % recovery (n = 5) is 101.05 ± 1.65. A proposal of the reaction pathway is presented.

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Determination of amoxapine and nortriptyline in blood plasma and serum by salt‐assisted liquid–liquid microextraction and high‐performance liquid chromatography

Gupta

Jain

Verma

2010

J of Separation Science

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A new, selective and sensitive method has been developed for the determination of tricyclic antidepressant drugs, amoxapine and nortriptyline, in human blood plasma and serum, involving their reaction with allyl isothiocyanate and extraction of thiourea derivatives with water-miscible organic solvent acetonitrile. The phase separation was effected by addition of ammonium sulphate, a process called salt-assisted liquid-liquid microextraction. The extract was analyzed by HPLC with UV detection at 254 nm. The method has been optimized for derivatization reaction time and temperature, solvent for extraction, and salt for solvent phase separation. Under the optimal conditions, a linear calibration graph was obtained between the amount of drug and the peak area of thiourea derivatives in the range of 0.002-20 mg/L drugs. The correlation coefficient and limit of detection values for amoxapine and nortriptyline in serum/plasma samples were in the range of 0.9953-0.9999 and 0.46-0.58 μg/L, respectively. The recovery in spiking experiments ranged, respectively, 75-88% (RSD 3.4-7.2%) and 79-97% (RSD 3.7-7.9%) for the two drugs.

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Determination of tianeptine in human plasma using high-performance liquid chromatography with fluorescence detection

Cited by 20 publications

References 11 publications

Development and validation of an HPLC method for the determination of benzodiazepines and tricyclic antidepressants in biological fluids after sequential SPE

Development and validation of an HPLC method for the determination of benzodiazepines and tricyclic antidepressants in biological fluids after sequential SPE

Spectrofluorimetric and Spectrophotometric Determination of Oxamniquine in Pharmaceuticals and Biological Fluids via Derivatization with 4‐Chloro‐7‐Nitrobenzo‐2‐oxa‐1,3‐Diazole (NBD‐Cl)

Determination of amoxapine and nortriptyline in blood plasma and serum by salt‐assisted liquid–liquid microextraction and high‐performance liquid chromatography

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