Determination of Tiopronin in Pharmatceutical Preparations by Time Resolved Chemiluminescence Using the Stopped-Flow Technique

Determination of tiopronin content in drug by copper(I)-cuproine spectrophotometry

“…In comparison to literature reports [3][4][5][6][7][8][9][10], the proposed method does not need complicated or expensive instrument, and the procedure of proposed method is simple and convenient.…”

“…Therefore, the determination of tiopronin content is of great importance. Up to now, spectrophotometry [1][2], kinetic spectrophotometry [3][4], flow injection [5][6], chemiluminescence [7], electrochemiluminescence [8], LC-MS/MS [9] and LC-ESI-MS [10] have been used to determinate the content of TPN. In these literature, spectrophotometry is a commonly used method owing to its simplicity, rapidity and cheap instrument.…”

“…In these literature, spectrophotometry is a commonly used method owing to its simplicity, rapidity and cheap instrument. In the literatures [3][4][5][6][7][8][9][10], complicated or expensive instruments are required, so the use of these methods is limited. The hydrosulfuryl(-SH) in tiopronin can quantitatively reduce Cu 2+ to Cu + , Cu + react with cuproine to form copper(І)cuproine amaranth complex in the condition of pH=5.0.…”

Flow-injection Determination of Glutathione, Penicillamine and Tiopronin Based on the Reduction of Copper(II)-neocuproine Reagent

“…The British Pharmacopoeia recommend redox titration for GSH [7] and MPG, [8] and acidbase titration in non-aqueous media for PEN. [9] Other reported methods for determination of these RSH in pharmaceuticals include spectrophotometry, [10][11][12][13][14] fluorimetry, [15][16][17] chemiluminescence, [18][19][20] electroanalytical [21][22][23] and chromatographic techniques. [24][25][26] Flow injection analysis (FIA) became a versatile instrument tool for the quality control of pharmaceuticals in the 21 st century.…”

Determination of penicillamine, tiopronin and glutathione in pharmaceutical formulations by kinetic spectrophotometry

“…Official methods (from the British Pharmacopoeia) for determination of these thiol compounds (RSH) are acid-base titration in a non-aqueous medium for PEN and redox titration for MPG and GSH (8). Other reported methods for determination of these RSH compounds in pure form and pharmaceutical formulations include spectrophotometry (9)(10)(11)(12)(13)(14)(15), fluorimetry (16)(17)(18)(19) chemiluminescence (20)(21)(22), electroanalytical techniques (23)(24)(25)(26)(27), chromatography (28)(29)(30) and flow techniques (31)(32)(33)(34)(35).…”