Flow-injection Determination of Glutathione, Penicillamine and Tiopronin Based on the Reduction of Copper(II)-neocuproine Reagent

Kukoc-Modun, Lea; Biocic, Maja; Radić, Njegomir

doi:10.5562/cca3688

Cited by 6 publications

(9 citation statements)

References 28 publications

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“…A sample of pure 20 µg.mL -1 D-PEN spiked with half, equal and double fold excess concentration of selected interferences and 1.3×10 -3 M NQS in presence of 0.2M of sodium phosphate buffer, three excipients were analyzed. The acceptable recovery values (95-105%) demonstrated that, there were no interferences during the determination of D-PEN using new CFIA system, the results summarized in Table (7).…”

Section: Study Of Interferencesmentioning

confidence: 80%

“…The optimum concentration of the reagent NQS was studied by injecting different concentrations (3.3×10 -4 -5.4×10 -3 ) M using a homemade injection valve loading in (L2). The results in Figure (7) indicated that the 1.3×10 -3 M gave the highest value of absorbance expressed as peak height in mV (n=3) with high repeatability.…”

Section: Effect Of Nqsmentioning

confidence: 95%

“…Several methods have been reported for the determination of D-PEN in pharmaceutical dosage forms including kinetic spectrophotometry [5], electrochemical chiral sensor [6], Flow-injection [7], Circular dichroism spectroscopy [8], spectrofluorimetric [9], Fluorometric sensor [10], HPLC [11,12], carbon paste electrode [13], HPLC-FLD and CE-LIF [14], spectrophotometric [15,16], potentiometric [17], fluorescence [18][19][20], Electrochemical sensor [21], Electrocatalytic [22], Chemiluminescence [23] and Voltametric [24].…”

Section: Introductionmentioning

confidence: 99%

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Development and Validation CFIA / MZ System as a Green Method for Determination of Thiol Drug (D-PEN)

Abdullah

Qassim

2021

Egypt. J. Chem.

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A simple, automated, and sensitive continuous flow-injection analysis/merging zones technique (CFIA/MZ) method was developed for the determination of D-Penicillamine (thiol drug) in pure, pharmaceutical formulations and biological samples. This method involved the reaction of 1,2-Naphthoquinone-4-Sulphonic acid Sodium Salt (NQS) with D-Penicillamine to produce a brown-colored complex that has maximum absorbance at 463 nm. The method was cheap, economic, precise and accurate where the limit of detection was 6.3 µg.mL -1 and the RSD% was 0.34 and Recovery was 99.31%. Various chemical and physical conditions that affected the reaction have been studied. A wide calibration curve was rectilinear within the concentration range (15-1000) μg.mL -1 with a sample throughput of 103 samples.hour -1 . The proposed procedure was applied successfully for the estimation of D-PEN in pharmaceutical and biological samples and the results obtained were favorably compared with those given by a reference method of United States Pharmacopoeia (USP), and there was no significant difference between the obtained results, regarding accuracy and precision at the 95% confidence level.

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Section: Study Of Interferencesmentioning

confidence: 80%

Section: Effect Of Nqsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Development and Validation CFIA / MZ System as a Green Method for Determination of Thiol Drug (D-PEN)

Abdullah

Qassim

2021

Egypt. J. Chem.

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“…In contrast, studies have found that L-penicillamine (L-Pen) is toxic and can cause neuritis and marrow damage [ 24 , 25 ]. Currently, the methods for detection and isolation of penicillamine involve flow injection [ 26 ], chromatography [ 27 , 28 , 29 ], spectrometry [ 30 , 31 , 32 ], colorimetric method [ 33 ], and electrochemical method [ 34 , 35 , 36 , 37 ]. However, the electrochemical sensor is miniaturized and easy to prepare, which effectively avoids the defects such as complex sample preparation, long detection time and expensive chiral selector.…”

Section: Introductionmentioning

confidence: 99%

DNA-Immobilized Special Conformation Recognition of L-Penicillamine Using a Chiral Molecular Imprinting Technique

et al. 2022

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A new chiral molecularly imprinted polymer (MIP) sensor with dual recognition ability was developed for the highly selective separation of enantiomers with toxic side effects in drugs. The sensor contains double-stranded deoxyribonucleic acid (dsDNA) as the element that immobilizes the chiral molecular conformation: the dsDNA enables the imprinted cavities to match the three-dimensional structure and functional groups from the chiral molecule. By embedding the spatial orientation of dsDNA in MIPs, one can accurately capture and immobilize the molecular conformation, eliminating the influence of interfering analogues. Herein, L-penicillamine (L-Pen) was selected as the chiral template molecule and embedded into dsDNA to form dsDNA-L-Pen complex, which was then embedded into the MIPs by electropolymerization. After elution, the stereo-selective imprinted cavities were obtained. The ATATATATATAT-TATATATATATA base sequence showed a high affinity for the embedded L-Pen, which endowed the imprinted cavities with a larger number of sites and improved the selectivity toward Pen enantiomers. Under the optimal working conditions, the current response of the MIP/dsDNA sensor exhibited a positive linear relationship with the logarithm of the L-Pen concentration in the range of 3.0 × 10−16 to 3.0 × 10−13 mol/L, and the detection limit was 2.48 × 10−16 mol/L. After the introduction of dsDNA into the MIP, the selectivity of the sensor toward D-Pen increased by 6.4 times, and the sensor was successfully applied in the analysis of L-Pen in penicillamine tablets.

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“…In light of its important biological and clinical significance, there have been increasing interest in developing analytical methods for GSH determination ( Steghens et al, 2003 ). GSH-recycling assay ( Tietze, 1969 ; Chowdhury et al, 2013 ) with commercial assay kits, fluorescence ( Cai et al, 2015 ), electrochemistry ( Gao et al, 2016 ), surface-enhance Raman spectroscopy (SERS) ( Saha and Jana, 2013 ; Zhu et al, 2021 ), colorimetry ( Lee et al, 2018 ), flow injection analysis ( Zitka et al, 2007 ; Kukoc-Modun et al, 2020 ), and high-performance liquid chromatography–mass spectrometry (HPLC–MS) ( Moore et al, 2013 ) have been documented. Among these methods, HPLC–MS has been a method of choice for the determination of GSH due to its advantages of sensitivity and specificity ( Moral et al, 2012 ; Avula et al, 2013 ; Moore et al, 2013 ; Sun et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Combination of Facile Thiol-Maleimide Derivatization and Supramolecular Solvent-Based Microextraction for UHPLC–HRMS Analysis of Glutathione in Biofluids

et al. 2021

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Glutathione (GSH) is the most abundant non-protein thiol in biofluids, enabling diverse physiological functions. Among the proposed methods for GSH detection, ultra-high-performance liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) has the advantages of high sensitivity and efficiency. In this study, a novel analytical method was developed for the determination of GSH using supramolecular solvent (SUPRAS)-based dispersive liquid–liquid microextraction (DLLME) and UHPLC–HRMS. N-Laurylmaleimide was dissolved in tetrahydrofuran, which served three functions: 1) precipitate the proteins present in the biofluid sample, 2) provide a reaction environment for derivatization, and 3) enable the use of SUPRAS as the dispersing agent. Critical parameters were optimized based on single factor testing and response surface methodology. The established method was validated in terms of linearity, accuracy, precision, and successful quantitative analysis of GSH in saliva, urine, and plasma samples. Experimental results showed that SUPRAS as an extraction solvent was particularly suitable for the extraction of GSH from complex matrices. The current study provides a useful tool for accurate measurements of GSH concentrations, which could potentially be used for clinical diagnostics.

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Flow-injection Determination of Glutathione, Penicillamine and Tiopronin Based on the Reduction of Copper(II)-neocuproine Reagent

Cited by 6 publications

References 28 publications

Development and Validation CFIA / MZ System as a Green Method for Determination of Thiol Drug (D-PEN)

Development and Validation CFIA / MZ System as a Green Method for Determination of Thiol Drug (D-PEN)

DNA-Immobilized Special Conformation Recognition of L-Penicillamine Using a Chiral Molecular Imprinting Technique

Synergistic Combination of Facile Thiol-Maleimide Derivatization and Supramolecular Solvent-Based Microextraction for UHPLC–HRMS Analysis of Glutathione in Biofluids

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