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Nitro Schiff base ligand of 2-(biphenyl-4-yl)-3-((2-(2,4-dinitrophenyl) hydrazono) methyl) imidazo [1,2-a] pyridine (BDNHMIP) was synthesized, identified and used in spectrophotometric determination of Co(II) at 530nm. The suggested method of Co(II) determination was performed at optimum analytical parameters of 3.2 ml/min flow rate, 137.4 L ligand volume, 98.1 L Co(II) volume, 1minutes residence time in reaction coil, and as a result 48 sample/hour of method throughput was accomplished. The proposed method performed linearity of 25-400 ppm, molar absorptivity coefficient of 121.570410 3 L.mol. 1 cm 1 , and L.O.D of 2.28ppm. The suggested method was successful applied for determination of Co(II) content of vitamin B 12 injection samples with high recovery of 99.5-100.3% and minimum relative error percent of-0.3-0.5%.

A simple, automated, and sensitive continuous flow-injection analysis/merging zones technique (CFIA/MZ) method was developed for the determination of D-Penicillamine (thiol drug) in pure, pharmaceutical formulations and biological samples. This method involved the reaction of 1,2-Naphthoquinone-4-Sulphonic acid Sodium Salt (NQS) with D-Penicillamine to produce a brown-colored complex that has maximum absorbance at 463 nm. The method was cheap, economic, precise and accurate where the limit of detection was 6.3 µg.mL -1 and the RSD% was 0.34 and Recovery was 99.31%. Various chemical and physical conditions that affected the reaction have been studied. A wide calibration curve was rectilinear within the concentration range (15-1000) μg.mL -1 with a sample throughput of 103 samples.hour -1 . The proposed procedure was applied successfully for the estimation of D-PEN in pharmaceutical and biological samples and the results obtained were favorably compared with those given by a reference method of United States Pharmacopoeia (USP), and there was no significant difference between the obtained results, regarding accuracy and precision at the 95% confidence level.

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