Determination of total and oxidized glutathione in human whole blood with a sequential injection analysis system

Araújo, André R. T. S.; Saraiva, M. Lúcia M.F.S.; Lima, José L.F.C.

doi:10.1016/j.talanta.2007.09.028

Cited by 40 publications

(27 citation statements)

References 28 publications

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“…It is based on the progressive reduction of GSSG to GSH with GR in the presence of NADPH, while the rate of formation of the colored product 5-thionitrobenzoate is measured. DTNB has been employed in several studies for the determination of both GSH and GSSG [58][59][60][68][69][70], in the first three ones coupled with NEM. 4,4 -Dithiodipyridine (4-DPS) has been used for the quantification of protein thiols and dithiols in picomolar amounts.…”

Section: Derivatization Of Free and Bound Thiol Functionalities In Glmentioning

confidence: 99%

Determination of glutathione and glutathione disulfide in biological samples: An in-depth review

Peter¹,

Wittmann²,

Karg³

et al. 2009

Journal of Chromatography B

247

153

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Section: Derivatization Of Free and Bound Thiol Functionalities In Glmentioning

confidence: 99%

Determination of glutathione and glutathione disulfide in biological samples: An in-depth review

Peter¹,

Wittmann²,

Karg³

et al. 2009

Journal of Chromatography B

247

153

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“…Erythrocyte GSH (RBC GSH) serves as a convenient biological reserve in which to measure GSH status, and several valid measurement methods are available, with enzymatic recycling being the most sensitive. [15][16][17][18] Importantly, the reactive nature of GSH causes challenges in sample stability and necessitates correct sample collection methods. 19 The bioavailability of orally administered cysteine is believed to be the rate-limiting step for the synthesis of glutathione, 10 although the amino acids glutamate/ glutamine are also integral to GSH synthesis.…”

Section: Introductionmentioning

confidence: 99%

Effects of Oral Glutathione Supplementation on Systemic Oxidative Stress Biomarkers in Human Volunteers

Allen

Bradley

2011

The Journal of Alternative and Complementary Medicine

136

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Background: The tripeptide glutathione (GSH) is the most abundant free radical scavenger synthesized endogenously in humans. Increasing mechanistic, clinical, and epidemiological evidence demonstrates that GSH status is significant in acute and chronic diseases. Despite ease of delivery, little controlled clinical research data exist evaluating the effects of oral GSH supplementation. Objectives: The study objectives were to determine the effect of oral GSH supplementation on biomarkers of systemic oxidative stress in human volunteers. Design: This was a randomized, double-blind, placebo-controlled clinical trial. Setting/location: The study was conducted at Bastyr University Research Institute, Kenmore, WA and the Bastyr Center for Natural Health, Seattle, WA. Subjects: Forty (40) adult volunteers without acute or chronic disease participated in this study. Intervention: Oral GSH supplementation (500 mg twice daily) was given to the volunteers for 4 weeks. Outcome measures: Primary outcome measures included change in creatinine-standardized, urinary F2-isoprostanes (F2-isoP) and urinary 8-hydroxy-2¢-deoxyguanosine (8-OHdG). Changes in erythrocyte GSH concentrations, including total reduced glutathione (GSH), oxidized glutathione (GSSG), and their ratio (GSH:GSSG) were also measured by tandem liquid chromatography/mass spectrometry. Analysis of variance was used to evaluate differences between groups. Results: There were no differences in oxidative stress biomarkers between treatment groups at baseline. Thirtynine (39) participants completed the study per protocol. Changes in creatinine standardized F2-isoP (ng/mg creatinine) (0.0 -0.1 versus 0.0 -0.1, p = 0.38) and 8-OHdG (lg/g creatinine) (-0.2 -3.3 versus 1.0 -3.2, p = 0.27) were nonsignificant between groups at week 4. Total reduced, oxidized, and ratio measures of GSH status were also unchanged. Conclusions: No significant changes were observed in biomarkers of oxidative stress, including glutathione status, in this clinical trial of oral glutathione supplementation in healthy adults.

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“…Such methods are typically simple, easy to handle, with low operational costs and with satisfactory figures of merit for most quality control applications [19]. The main analytical figures of merit of spectrophotometric SI methods for the determination of thiols can be found in Table 1 [20][21][22][23][24][25][26][27].…”

Section: Determination Of Thiols By Si-spectrophotometrymentioning

confidence: 99%

Automated Determination of Pharmaceutically and Biologically Active Thiols by Sequential Injection Analysis: A Review

Tzanavaras

2010

TOCBMJ

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Abstract:The present review article presents and discusses automated sequential injection (SI) based methods for the determination of pharmaceutically and biologically important thiols, namely cysteine, N-acetylcysteine, penicillamine, glutathione and captopril. The review intends to cover a range of ten years of published research on this topic (2000 -2009). The methods are classified according to the detection systems in three categories, namely spectrophotometric, fluorimetric and electroanalytical. The determination principles of the methods are discussed and the main analytical figures of merit are tabulated.

show abstract

Determination of total and oxidized glutathione in human whole blood with a sequential injection analysis system

Cited by 40 publications

References 28 publications

Determination of glutathione and glutathione disulfide in biological samples: An in-depth review

Determination of glutathione and glutathione disulfide in biological samples: An in-depth review

Effects of Oral Glutathione Supplementation on Systemic Oxidative Stress Biomarkers in Human Volunteers

Automated Determination of Pharmaceutically and Biologically Active Thiols by Sequential Injection Analysis: A Review

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