Determination of total and speciated arsenic in rice by ion chromatography and inductively coupled plasma mass spectrometry

Heitkemper, Douglas T.; Vela, Nohora P.; Stewart, Kirsten R.; Westphal, Cédric

doi:10.1039/b007241i

Cited by 309 publications

(212 citation statements)

References 36 publications

Supporting

Mentioning

188

Contrasting

Unclassified

Order By: Relevance

“…The As speciation of NIST 1568a rice flour reference material has been repeatedly characterized and was used to validate our arsenic speciation technique. Our results are in agreement with previously published data (8,(16)(17)(18).…”

Section: Introductionsupporting

confidence: 94%

Increase in Rice Grain Arsenic for Regions of Bangladesh Irrigating Paddies with Elevated Arsenic in Groundwaters

Williams

Islam

Adomako

et al. 2006

Environ. Sci. Technol.

491

319

View full text Add to dashboard Cite

Concern has been raised by Bangladeshi and international scientists about elevated levels of arsenic in Bengali food, particularly in rice grain. This is the first inclusive food market-basket survey from Bangladesh, which addresses the speciation and concentration of arsenic in rice, vegetables, pulses, and spices. Three hundred thirty aman and boro rice, 94 vegetables, and 50 pulse and spice samples were analyzed for total arsenic, using inductivity coupled plasma mass spectrometry (ICP-MS). The districts with the highest mean arsenic rice grain levels were all from southwestern Bangladesh: Faridpur (boro) 0.51 > Satkhira (boro) 0.38 > Satkhira (aman) 0.36 > Chuadanga (boro) 0.32 > Meherpur (boro) 0.29 µg As g -1 . The vast majority of food ingested arsenic in Bangladesh diets was found to be inorganic; with the predominant species detected in Bangladesh rice being arsenite (As III ) or arsenate (As V ) with dimethyl arsinic acid (DMA V ) being a minor component. Vegetables, pulses, and spices are less important to total arsenic intake than water and rice. Predicted inorganic arsenic intake from rice is modeled with the equivalent intake from drinking water for a typical Bangladesh diet. Daily consumption of rice with a total arsenic level of 0.08 µg As g -1 would be equivalent to a drinking water arsenic level of 10 µg L -1 .

show abstract

Section: Introductionsupporting

confidence: 94%

Increase in Rice Grain Arsenic for Regions of Bangladesh Irrigating Paddies with Elevated Arsenic in Groundwaters

Williams

Islam

Adomako

et al. 2006

Environ. Sci. Technol.

491

319

View full text Add to dashboard Cite

show abstract

“…Although no experiment for arsenate uptake from polluted soils was carried out during the present study, similar results as those of Abadan are expected especially with the fact that arsenate is the dominant species in the tested Egyptian soil of El-Sharkeya Governorate [1]. It was shown that MMAA and DMAA can be taken up by rice roots at a slow rate, as a result and due to the restricted translocation of MMAA from roots to the areal parts of plant while DMAA could be a major component of total As in rice grains [26]. Rice cultivars tested in the present study showed variable resistance to As with the variant Sakha 104 being more efficient to express arsenate resistant genes than the other tested cultivars.…”

Section: Discussionsupporting

confidence: 73%

Arsenate-Resistant Genes in Egyptian Rice Cultivars as Soil Pollution Sensors

Hafez¹,

Bestawy²,

Rashad³

et al. 2017

Preprint

View full text Add to dashboard Cite

The main objective of the present study was to investigate arsenate [As (V)] resistance genes in rice cultivars grown in arsenic contaminated Egyptian soil in order to genetically induce resistance against arsenic in the local rice varieties as well as defining contaminated rice grains and/or soil. Three local rice cultivars; Sakha 102-104 were cultivated on modified Murashige and Skoog Basal Medium (MS medium) containing elevated concentrations of arsenate (0.1, 1 and 10 mg/l). The three varieties showed different resistant attitudes against arsenate with Sakha 104 being the most resistant. Extracted messenger RNA (mRNA) from treated and untreated Sakha 104 plantlets was scanned using differential display to demonstrate the arsenate resistant genes using three different arbitrary primers. About 100 different RNAs with (1500 bp -50 bp) were obtained from which seven were up-regulated genes, subjected to DNA cloning using TOPO TA system and the selected clones were sequenced. The sequence analysis described four genes out of the seven namely disease resistance protein RPM1, Epstein-Barr virus EBNA-1-like, CwfJ family protein and outer membrane lipoprotein OmlA while the other three genes were hypothetical proteins. It is concluded the four induced genes in the resistant rice cultivar considered as a direct response to arsenic soil pollution. Genes detected in the present study can be used as geno-sensors for rice grains and soil contamination with As (V). Moreover, local rice cultivars may be genetically modified with such genes to induce high resistance and to overcome arsenic soil pollution.

show abstract

“…The chromatographic system consisted of a Dionex GP 50 pump Environmental and Molecular Mutagenesis. DOI 10.1002/em with a 200 lL sample loop, a Dionex AS 50 autosampler, and a Hamilton PRP-X100 anion exchange column [Heitkemper et al, 2001;Wang et al, 2007b;Ketavarapu et al, 2008]. At the beginning of each chromatographic run 50 lL of a 10 lg As V L 21 solution was injected to correct for any signal sensitivity drift that occurred over the duration of sample batch time.…”

Section: Speciation Of Arsenic In Cell Culture Supernatantsmentioning

confidence: 99%

Evaluation of gene expression changes in human primary uroepithelial cells following 24‐Hr exposures to inorganic arsenic and its methylated metabolites

Yager

Gentry

Thomas

et al. 2012

Environ and Mol Mutagen

View full text Add to dashboard Cite

Gene expression changes in primary human uroepithelial cells exposed to arsenite and its methylated metabolites were evaluated to identify cell signaling pathway perturbations potentially associated with bladder carcinogenicity. Cells were treated with mixtures of inorganic arsenic and its pentavalent or trivalent metabolites for 24 hr at total arsenic concentrations ranging from 0.06 lM to 18 lM. One series (five samples) was conducted with arsenite and pentavalent metabolites and a second (10 samples) with arsenite and trivalent metabolites. Similar gene expression responses were obtained for pentavalent or trivalent metabolites. A suite of eight gene changes was consistently identified across individuals that reflect effects on key signaling pathways: oxidative stress, protein folding, growth regulation, metallothionine regulation, DNA damage sensing, thioredoxin regulation, and immune response. No statistical significance of trend (NOSTASOT) analysis of these common genes identified lowest observed effect levels (LOELs) from 0.6 to 6.0 lM total arsenic and no observed effect levels (NOELs) from 0.18 to 1.8 lM total arsenic. For the trivalent arsenical mixture, benchmark doses (BMDs) ranged from 0.13 to 0.92 lM total arsenic; benchmark dose lower 95% confidence limits (BMDLs) ranged from 0.09 to 0.58 lM total arsenic. BMDs ranged from 0.53 to 2.7 lM and BMDLs from 0.35 to 1.7 lM for the pentavalent arsenical mixture. Both endpoints varied by a factor of 3 across individuals. This study is the first to examine gene expression response in primary uroepithelial cells from multiple individuals and to identify no effect levels for arsenical-induced cell signaling perturbations in normal human cells exposed to a biologically plausible concentration range.Environ. Mol. Mutagen. 54:82-98, 2013. V V C 2012 Wiley Periodicals, Inc.

show abstract

Determination of total and speciated arsenic in rice by ion chromatography and inductively coupled plasma mass spectrometry

Cited by 309 publications

References 36 publications

Increase in Rice Grain Arsenic for Regions of Bangladesh Irrigating Paddies with Elevated Arsenic in Groundwaters

Increase in Rice Grain Arsenic for Regions of Bangladesh Irrigating Paddies with Elevated Arsenic in Groundwaters

Arsenate-Resistant Genes in Egyptian Rice Cultivars as Soil Pollution Sensors

Evaluation of gene expression changes in human primary uroepithelial cells following 24‐Hr exposures to inorganic arsenic and its methylated metabolites

Contact Info

Product

Resources

About