Determination of trace macrolide antibiotics in milk with online solid‐phase extraction with an ionic‐liquid‐based monolithic column

Abstract

An ionic liquid (IL)-based monolithic poly(ionic liquid glycidylmethacrylate-co-ethylene glycol dimethacrylate) column was prepared via in situ free-radical polymerization with 1-vinyl-3-butylimidazolium chlorine as one of the comonomers. The obtained monolithic column was used as the sorbent of solid-phase extraction (SPE) and coupled with high-performance liquid chromatography for the simultaneous determination of the macrolide antibiotics roxithromycin (ROX) and acetylspiramycin (ACE) in processed pure milk. The monolithic column was characterized by Fourier transform infrared spectrometry, scanning electron microscopy, nitrogen absorption-desorption, mercury intrusion porosimetry, and thermogravimetric analysis. The results reveal that the monolithic column exhibited a high selectivity and good permeability to the macrolide antibiotics in milk. The optimized method offered excellent linearity with a linear regression coefficient greater than 0.998. The precisions for interday and intraday were both less than 7.7%. The accuracies expressed by the recoveries for ROX and ACE were in the ranges 92.5-103.8 and 93.0-107.6%, respectively. Compared to the previous methods, this method had a low limit of detection and a good accuracy. As a result, the polymer IL based monolithic column could feasibly be used as a high-selectivity online SPE sorbent for determining trace macrolide antibiotics in milk.

Developing a novel magnetic organic polymer for selective extraction and determination of 16 macrolides in water and honey samples

“…Therefore, sample pretreatment is often an essential procedure prior to instrumental analysis. To date, there are many sample pretreatment methods for MALs, such as liquid–liquid extraction (LLE), 31 dispersive liquid–liquid microextraction (DLLME), 32 solid-phase extraction (SPE), 33 dispersive solid-phase extraction (DSPE) 34 and magnetic solid-phase extraction (MSPE). 35 Compared to other methods, MSPE is an attractive technique due to its low consumption of organic solvents.…”