Determination of tranexamic acid in human serum by high-performance liquid chromatography using selective pre-column derivatization with phenyl isothiocyanate

Matsubayashi, Kyuichi; Kojima, Chieko; Tachizawa, Haruo

doi:10.1016/s0378-4347(00)80601-5

Cited by 27 publications

(19 citation statements)

References 9 publications

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“…The published analytical methods in the literature include reversed-phase HPLC with ultraviolet [3,4] or fluorescent detection [5,6], and gas chromatography (GC) [7]. However, these methods involve either a pre-column [3][4][5] or post-column [7] derivatization procedure, which is very cumbersome and not suitable for large number of sample determination. In addition, the complicated derivatization procedure may introduce large assay variations.…”

Section: Introductionmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

Chang

Yin

Chow

2004

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

Chang

Yin

Chow

2004

Journal of Chromatography B

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“…Plasma levels of 515-547 mM were reported [18] 5 min after intravenous bolus of 1000 mg TA. The proposed method seems simple for monitoring TA in plasma since HPLC analysis of TA in blood usually needs complicate procedures [19,20].…”

Section: Applicationmentioning

confidence: 99%

An ionizable chromophoric reagent for the analysis of primary amine-containing drugs by capillary electrophoresis

Lin

Kou

et al. 2005

Electrophoresis

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We found that ofloxacin acyl chloride is a potential chromophoric reagent for labeling amino analytes for capillary electrophoresis. Ofloxacin acyl chloride has a tertiary amino function in its structure and the derivatives from ofloxacin acyl chloride reacting with amino analytes can be ionized by an acid treatment and analyzed by simple capillary zone electrophoresis. Ofloxacin acyl chloride was used to derivatize model analytes (without chromophore) of amantadine (amino drug), tranexamic acid (non-protein amino carboxylic acid), glycine, and methionine (protein amino acids). The resulting derivatives were analyzed by capillary zone electrophoresis with ultraviolet detection (300 nm). The detection limits of the analytes studied were in the range of 1.0-2.5 microM (S/N = 3, injection 3 s). The precision (relative standard deviation) and accuracy (relative error) of the method for intra- and inter-day analyses of the analytes were respectively below 4.5% and 3.9%. Application of the method to the analysis of tranexamic acid in plasma proved feasible.

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“…Plasma ---The plasma concentration of tranexamic acid was determined by the modified method of Matsubayashi et al 14) Standard tranexamic acid (lot TSAAJ04) was supplied by Daiichi Seiyaku Co., Ltd., A 0.5 ml of plasma samples were added to 1.4 ml of ethanol and the contents was mixted on the vortex mixer. After centrifugation (15000 g, 5 min), the supernatant was added to 1 ml of 10 mM borax solution (pH 9.2) and 13 µl of phenyl isothiocyanate and kept at 40°C for 30 min.…”

Section: Determination Of Tranexamic Acid Levels Inmentioning

confidence: 99%

Comparison of the Effects of Cetraxate and its Major Metabolite on Human Plasma Gastrin, Somatostatin, Calcitonine Gene-Related Peptide and Substance P in Human Plasma

Katagiri

Inoue

Sato

et al. 2005

JOURNAL OF HEALTH SCIENCE

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Cetraxate hydrochloride (cetraxate), an antiulcer drug, produces a dose related increase in mucosal blood flow. We have reported that cetraxate increases plasma calcitonin gene-related peptide (CGRP) and substance P in healthy human subjects (J. Pharm. Pharmacol., 56, p. 557, 2004). Cetraxate is rapidly metabolized to tranexamic acid in plasma. We investigated the effect of tranexamic acid on human plasma CGRP, substance P, gastrin and somatostatin. Tranexamic acid at a dose of 500 mg or placebo was orally administered in five healthy male volunteers. The blood samples were taken before and at 20, 40, 60, 90, 120, 180 and 240 min after administrations, followed by the extracting procedure, and submitted to the high sensitive enzyme immunoassay system for CGRP and substance P as previously developed. Single administration of tranexamic acid caused significant increases of plasma CGRP concentration at 60-90 min compared with placebo, but the level-time profile was a little different from that of cetraxate. Tranexamic acid had no significant effect on plasma gastrin, somatostatin and substance P levels compared with placebo. In this study, we thought that the gastroprotective effect of cetraxate might not be due to plasma metabolite, tranexamic acid, but direct stimulation of gastric mucosa.

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Determination of tranexamic acid in human serum by high-performance liquid chromatography using selective pre-column derivatization with phenyl isothiocyanate

Cited by 27 publications

References 9 publications

Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

Liquid chromatography–tandem mass spectrometry method for the determination of tranexamic acid in human plasma

An ionizable chromophoric reagent for the analysis of primary amine-containing drugs by capillary electrophoresis

Comparison of the Effects of Cetraxate and its Major Metabolite on Human Plasma Gastrin, Somatostatin, Calcitonine Gene-Related Peptide and Substance P in Human Plasma

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