Determination of Tricyclazole Content in Paddy Rice by Surface Enhanced Raman Spectroscopy

Abstract

An ultrasensitive method based on Surface enhanced Raman scattering (SERS) has been developed to determine content of a pesticide which is tricyclazole in paddy rice using sliver colloid as a substrate and pyridine as an internal standard. The peaks at 424 and 1035 cm(-1) in a SERS spectrum were selected as analytic and internal peaks, respectively, and their intensity ratio I(t)/I(p) was used to calculate the regression concentration of tricyclazole. The correlation between I(t)/I(p) and concentration showed significant linear relationship with a correlation coefficient of R(2)= 0.995 in a concentration range of 0.05 to 0.70 mg/L and the tricyclazole solution can be detected to be low as 0.002 mg/L by SERS. The method was applied to determine tricyclazole contents of 3 real rice samples with a standard addition method in order to eliminate interference of matrix. The errors of SERS measurements for the 3 samples were 0.0008 to 0.0246, 0.0013 to 0.0028, and 0.0129 to 0.0304 mg/kg, respectively, compared with the results obtained by high performance liquid chromatography method. This also showed a good reproducibility with low values of relative standard deviation (n= 3) for the 3 samples ranged from 3.63% to 4.64%.

Surface‐Enhanced Raman Spectroscopy for the Chemical Analysis of Food

“…Therefore, there are many SERS studies of food analysis that are based on these substrates, especially favored by researchers in the chemistry/engineering areas (including food science) because of lack of capacity to fabricate sophisticated substrates. The use of Au and Ag colloids has been demonstrated in the detection of food additives, notably sodium benzoate, β‐hydroxy‐β‐methylbutanoic acid (HMB), L‐carnitine, N‐methylglycocyamine (Podstawka and others ), monosodium glutamate (Peica and others ), butylated hydroxyanisole (Yao and others ), Ponceau 4R (Xie and others ) and the chemical contaminants tricyclazole (Tang and others ), methamidophos (Xie and others ), omethoate, dimethoate (Guerrini and others ), nitrofurans (Xie and others ), melamine (Zhang and others ). The LOD varied from 2 ppb to 10 ppm, mainly depending on the composition and size of the colloids, chemical structure of analytes, and sample preparation and analysis procedures.…”

Fabrication process and characterization of AgNPs/PVA/cellulose as a SERS platform for in-situ detection of residual pesticides in fruit

“…However, the instability of the existing silver based substrates, leading to the requirement of strict conditions for preservation, has been the main issue limiting the utility of SERS in practice. Several stabilizers have been investigated, such as poly(N-vinylpyrrolidone) (PVP), carboxylic acid, sodium citrate, nano cellulose, starch, and protein [5][6][7][8][9]. The use of stabilizers, while improving the stability, also interfere with the scattering signals, which is extremely undesirable especially in quantitative analysis.…”

Facile and Sensitive Detection of Carbofnran Carbamate Pesticide in Rice and Soybean Using Coupling Reaction-based Surface-Enhanced Raman Scattering

“…For example, Vongsvivut et al 20 offered the application of the SERS technique for the trace detection of fonofos pesticide adsorbed on silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs). Tang et al 21 applied SERS to develop a sensitive and practical protocol for the analysis of the tricyclazole content in paddy rice. Zhang et al 22 created a self-assembled protocol to fabricate a AgNPs monolayer film as a SERS substrate for methyl-parathion detection.…”