Determination of two different types of cellular cementogenesis in rat molars: A histological and immunohistochemical study

Yamamoto, Tsuneyuki; Domon, Takanori; Takahashi, Shigeru; Arambawatta, A. K. S.; Anjuman, K A Y; Fukushima, Chifumi; Wakita, Minoru

doi:10.1016/j.matbio.2005.04.003

Cited by 5 publications

(7 citation statements)

References 46 publications

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“…The evaluation of fresh-frozen sections Regarding the immunohistochemistry for BSP and OPN, the acellular cementum in fresh-frozen sections stained more intensely and specifically than in chemically fixed, EDTA-demineralized, paraffin sections (see Hosoya et al 2003;Yamamoto et al 2004Yamamoto et al , 2005.…”

Section: Discussionmentioning

confidence: 99%

“…We have studied the structure and development of the acellular cementum in rat molars by light and electron microscopy (Yamamoto 1986;Yamamoto and Wakita 1990;Yamamoto et al 2000aYamamoto et al , 2000bYamamoto et al , 2001Yamamoto et al , 2004Yamamoto et al , 2005, and found the following: (1) the cemento-dentinal junction is a fibril-poor layer (1-2 μm thick) and contains more densely accumulated BSP and OPN than the successively formed acellular cementum with extrinsic fibers; (2) developmentally, prior to the principal fiber attachment to the root surface, BSP and OPN are densely deposited as the initial cementum, which results in the fibril-poor cemento-dentinal junction; (3) during the initial cementogenesis, matrix vesicles and calciferous spherules appear on the root surface.…”

Section: The Mineralization Of Acellular Cementummentioning

confidence: 99%

“…For rat molars, it is generally accepted that the cemento-dentinal junction is fibril-poor and contains more highly accumulated BSP and OPN than other parts of cementum (Paynter and Pudy 1958;Yamamoto 1986;Yamamoto and Wakita 1990;Yamamoto et al 2000aYamamoto et al , 2000bYamamoto et al , 2001Yamamoto et al , 2004Yamamoto et al , 2005Sasano et al 2001;Tadatomo et al 2002;Hosoya et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Yamamoto

Domon

Takahashi

et al. 2006

Histochem Cell Biol

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This study was designed to detect tissue non-specific alkaline phosphatase (TNSALP) by Azo-dye staining, calcium by glyoxal bis(2-hydroxyanil) (GBHA) staining, bone sialoprotein (BSP) and osteopontin (OPN) by immunoperoxidase staining in developing rat molars, and also to discuss the mineralization process during acellular cementogenesis. To restrain a reduction in histochemical and immunohistochemical reactions, fresh-frozen undemineralized sections were prepared. Where the epithelial sheath was intact, TNSALP reaction was observed in the dental follicle, but not in the epithelial sheath. With the onset of dentin mineralization, the BSP-and OPN-immunoreactive, initial cementum layer appeared. At this point cementoblasts had shown intense TNSALP reaction and GBHA reactive particles (=calcium-GBHA complex) appeared on the root surface. With further development, the reaction of TNSALP and GBHA became weak on the root surface. Previous studies have shown that the initial cementum is fibril-poor and that matrix vesicles and calciferous spherules appear on the root surface only during the initial cementogenesis. The findings mentioned above suggest that: During the initial cementogenesis cementoblasts release matrix vesicles which result in calciferous spherules, corresponding to the GBHA reactive particles. The calciferous spherules trigger the mineralization of the initial cementum. After principal fiber attachment mineralization advances along collagen fibrils without matrix vesicles.

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Section: Discussionmentioning

confidence: 99%

Section: The Mineralization Of Acellular Cementummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Yamamoto

Domon

Takahashi

et al. 2006

Histochem Cell Biol

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“…Human and rat cementum contains two types of non-collagenous acid glycoproteins, bone sialoprotein (BSP) and osteopontin (OPN) (12,18,24), which accumulate especially at the cemento-dentinal junction (7,(25)(26)(27). Densely accumulated BSP and OPN at the cemento-dentinal junction have been suggested to be involved in the induction of cementogenesis and in the firm adhesion of cementum and dentin in rat and human teeth (2,7,11,(25)(26)(27). Therefore, these glycoproteins could be used for new indices to evaluate cementum regeneration.…”

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confidence: 99%

Immunohistochemical analysis of periodontal reattachment on denuded root dentin after periodontal surgery

et al. 2010

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This study evaluates the effects of three kinds of periodontal surgery using statistical analyses of histological and immunohistochemical indices. Dehiscence defects were made on roots of maxillary teeth in monkeys. Surgically exposed roots were untreated, etched with EDTA, or treated with Emdogain ® after EDTA etching. Paraffin sections were stained with hematoxylin and eosin or immunostained for bone sialoprotein (BSP) or osteopontin (OPN) and analysed using several indices. The relative length of regenerated cementum and of BSP/OPN-immunoreactive lines on dentin defect showed no differences among the three groups. The attached regenerated cementum in the untreated group was shorter than in the etching groups. Regenerated cementum area was larger in the etching-Emdogain group than in the etching group. Thickness of immunolabeling on detached cementum was larger than that on attached cementum in all of the groups. These findings suggest that etching reinforces the attachment of regenerated cementum, and that BSP and OPN are associated with the attachment, where they exercise strong adhesion within a certain level of thickness.

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“…Therefore, sections stained with antibodies purchased from LSL are shown in the figures. The authenticity of these antibodies to rats has been established in previous studies (19,31,34).…”

Section: Immunohistochemistrymentioning

confidence: 85%

An immunohistochemical study of the attachment mechanisms in different kinds of adhesive interfaces in teeth and alveolar bone of the rat

Arambawatta

Yamamoto

Wakita

2006

J of Periodontal Research

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The above findings suggest that (i) the different types of adhesive interfaces in the rat molar region have a common structure in that they are filled with highly accumulated bone sialoprotein and osteopontin and deficient in collagen fibrils; (ii) accumulated bone sialoprotein and osteopontin are closely associated with the adhesion at the interfaces; and (iii) the adhesive interfaces have a similar developmental process.

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Determination of two different types of cellular cementogenesis in rat molars: A histological and immunohistochemical study

Cited by 5 publications

References 46 publications

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Mineralization process during acellular cementogenesis in rat molars: a histochemical and immunohistochemical study using fresh-frozen sections

Immunohistochemical analysis of periodontal reattachment on denuded root dentin after periodontal surgery

An immunohistochemical study of the attachment mechanisms in different kinds of adhesive interfaces in teeth and alveolar bone of the rat

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