Determination of unsaturated fatty acids with a migrating double bond in complex biological matrices by gas chromatography with flame ionization and mass spectrometry detection

Ivankin, Andrey; Oliferenko, G. L.; Куликовский, А. Г.; Чернуха, И. М.; Semenova, Anastasia; Spiridonov, K. I.; Насонова, В.В.

doi:10.1134/s1061934816110046

Cited by 9 publications

(3 citation statements)

References 24 publications

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“…Cholesterol determination was performed on an Agilent 7890A gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) with an Agilent 5975C mass spectrometric detector (Agilent Technologies, USA), an HP5MS capillary column (Agilent Technologies, USA) measuring 30 mm × 0.25 mm × 0.25 µm and helium as the carrier gas. Determination of the fatty acid composition and cholesterol content was performed according to the reported method in the literature [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Bioactive Compounds of Porcine Hearts and Aortas May Improve Cardiovascular Disorders in Humans

Чернуха

Kotenkova

Дербенева

et al. 2021

IJERPH

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Functional foods promote health benefits in human metabolism, with bioactive compounds acting as therapeutic agents. The aim was to investigate the biological effects of a pâté made of pork hearts and aortas, minced, sterilised and packed in tins. Adults (61–66 years old) with a body mass index of 26.4–60.7 kg/m2 (n = 36) were randomly divided into two groups: one group consumed a low-calorie diet (LCD), while the other consumed an LCD with the developed meat product (MP) for 28–30 days. Serum biochemical parameters, anthropometry and blood pressure were measured. Consumption of an LCD + MP by experimental group participants helped to maintain reduced cholesterol levels. The difference in total cholesterol was significantly different (p = 0.018) from that of the control group, mainly due to the difference in low-density lipoprotein cholesterol (p = 0.005). Six peptides with potential cholesterol-binding properties and four peptides with potential antioxidant activity were identified in the MP, while elevation of the content of two peptides with potential angiotensin-converting enzyme-inhibitory activity was detected in patients’ plasma. Intervention with the MP can be considered as a supportive therapy to the main treatment for medical cardiovascular diseases due to a positive effect on serum cholesterol.

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Section: Methodsmentioning

confidence: 99%

Bioactive Compounds of Porcine Hearts and Aortas May Improve Cardiovascular Disorders in Humans

Чернуха

Kotenkova

Дербенева

et al. 2021

IJERPH

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“…The fatty acid composition was determined using a flame ionization detector by the method of gas chromatography (Ivankin et al, 2016). For this, а 10-g weighed portion of a sample was treated for 3-24 h with a mixture of 10 mL of chloroform and 10 mL of methanol by the modified Folch method in the presence of a 1% KCl solution to dissolve the lipid component; the extract was filtered through paper and evaporated to dryness.…”

Section: Methodsmentioning

confidence: 99%

Effect of Sodium Chloride on Fat Oxidation in the Presence Of Heme Pigments

2019

CRPJFST

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The effects of salt on oxidative changes in fat and heme pigments were evaluated. The back fat was salted with sodium chloride in the presence of the hemoglobin. The indicators of the hydrolytic and oxidative spoilage were investigated in the samples of back fat from White Large pigs at two years of age. The proportions of heme and non-heme iron, as well as the content of metmyoglobin were determined in the samples of m. Longissimus dorsi from two-year-old female Large White pigs. Back fat salting did not significantly affect the acid value of back fat. Addition of salt in amounts of 3.5 % and 5.0 % initiated the oxidative changes. Addition of sodium chloride initiated fatty acid oxidation in the presence of the heme pigments. The content of unsaturated acids decreased by 22.8 % when 5.0 % of salt were added. The proportion of heme iron increased by 6.5 % relative to free iron when 5.0 % of sodium chloride were added. The positive correlation between the metmyoglobin content and the thiobarbituric acid value was found with the correlation coefficient of 0.98.

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“…The samples were obtained from at least three replicates from each carcass; average values of three replicates of fatty acid composition from each carcass were used for further data processing. Determination of the fatty acid composition was performed according to the reported method in the literature (Ivankin et al, 2016), with the author's modification. Results are available at (Chernukha et al, 2023).…”

Section: Sampling and Fatty Acids Analysismentioning

confidence: 99%

Principal Component Analysis and Cluster Analysis for Fatty Acid Assessment of Backfat in Three Pig Breeds

Chernukha,

Nikitina,

Kotenkova

et al. 2023

Meat Technology

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Determination of unsaturated fatty acids with a migrating double bond in complex biological matrices by gas chromatography with flame ionization and mass spectrometry detection

Cited by 9 publications

References 24 publications

Bioactive Compounds of Porcine Hearts and Aortas May Improve Cardiovascular Disorders in Humans

Bioactive Compounds of Porcine Hearts and Aortas May Improve Cardiovascular Disorders in Humans

Effect of Sodium Chloride on Fat Oxidation in the Presence Of Heme Pigments

Principal Component Analysis and Cluster Analysis for Fatty Acid Assessment of Backfat in Three Pig Breeds

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