Determination of urinary 8-hydroxydeoxyguanosine by automated coupled-column high performance liquid chromatography: a powerful technique for assaying in vivo oxidative DNA damage in cancer patients

Analysis of 8-hydroxy-2′-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry

“…The method has acceptable linearity, accuracy, and precision, and is more sensitive than previously described LC-MS/MS methods that have been used in reversed-phase columns. The proposed HILIC-MS/MS method is well suited for large-scale human studies and clinical studies, and would also be applicable to 14 analysis of 8-OHdG in not only urine but also other biological fluids such as plasma, serum and saliva, and in tissue. Table 1 Precision and accuracy in the determination of 8-OHdG in urine samples Table 1 Hosozumi et al …”

“…Measurements of 8-OHdG in urine samples are especially well-suited to large-scale human studies and clinical applications because they are noninvasive [10,11]. Urinary 8-OHdG has been analyzed by several methods, such as enzyme-linked immunosorbent assay (ELISA) [12,13], high-performance liquid 4 chromatography with electrochemical detection (HPLC-ECD) [14], gas chromatography with mass spectrometry (GC/MS) [15,16], and liquid chromatography with tandem mass spectrometry (LC/MS/MS) [17][18][19][20][21][22][23][24][25][26]. The ELISA method suffers from the problem of non-selectivity because the antibody may cross-react with other substances present in urine [12,27,28].…”

Determination of urinary oxidative DNA damage marker 8-hydroxy-2′-deoxyguanosine and the association with cigarette smoking

“…It's reported that within 8OHdG viral genomes introduce G:C → T:A transitions in vivo [10,11]. Due to its mutagenic potential, 8OHdG has been widely suggested as a biomarker for various cancers [12][13][14]. The concentration of 8OHdG is also evaluated in smokers and non-smokers [9,15,16] since cigarette smoke contains a large amount of oxygen radical forming substances, such as catechol and hydroquinone that may enhance lung carcinogenesis by free radical-mediated reactions.…”

Role of Chronic Inflammation and Resulting DNA Damage in Cervical Carcinogenesis Induced by Human Papillomavirus

“…Accumulation of 8-oxodG in human body has been demonstrated in patients with cancer and cancer-prone diseases. A significant increase in urinary 8-oxodG levels has been observed in patients with various types of cancer compared with those in control subjects (Erhola et al, 1997;Tagesson et al, 1995;Thanan et al, 2008). The content of 8-oxodG in cervical cells significantly increased with the grade of squamous intraepithelial lesion (Romano et al, 2000), raising the possibility that oxidative DNA damage participates in cervical carcinogenesis.…”