Determination of urinary prostaglandin E2 as a potential biomarker of ureteral stent associated inflammation

Huang, Zi‐Ao; Scotland, Kymora B.; Li, Yueyang; Tan, Jiahua; Kung, Sonia H.Y.; Chew, Ben H.; Chen, David D. Y.; Lange, Dirk

doi:10.1016/j.jchromb.2020.122107

Cited by 13 publications

(14 citation statements)

References 59 publications

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“…Other work has revealed hydronephrosis, hydroureter and ureteral dyskinesia in mice with Gli3 mutations (19). Our group has previously found a potential role for Gli1 in adult porcine ureteral peristalsis after ureteral stent implantation, which forms a partial obstruction (20). Here, we investigate the effect of Gli proteins, EPO and tamsulosin on the contractile ability of distal human ureteral tissue to better understand their roles in mediating smooth muscle contraction and peristalsis.…”

Section: Original Articlementioning

confidence: 91%

Mediators of human ureteral smooth muscle contraction—a role for erythropoietin, tamsulosin and Gli effectors

et al. 2021

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Background: Ureteral contractility is a poorly understood process. Contractions have been demonstrated to occur in the smooth muscle layers of the ureter. Previous work suggests the involvement of Gli family proteins and erythropoietin (EPO) in regulating mammalian ureteral smooth muscle contraction. We sought to devise a method by which the effects of these proteins and tamsulosin on distal human ureteral tissue contractility could be investigated to better understand mechanisms regulating human ureteral function.Methods: IRB approval was obtained to procure portions of extraneous distal ureteral tissue from living donor renal transplants. Contractility was measured by placing the tissue in Krebs buffer and stimulating via a uniform electric current. Contractile force was recorded with each stimulation with and without the presence of a Gli inhibitor (GANT61) or EPO. Each ureteral specimen was subsequently fixed and tested by immunohistochemistry to determine Gli, EPO and alpha-adrenergic receptor activity.Results: Electrical field stimulation successfully elicited contractions in the ureteral tissue. Administering tamsulosin decreased force and duration of ureteral contractions. Inhibiting Gli signaling decreased contractility and EPO decreased ureteral contractile forces within 5 minutes of administration versus untreated controls. Staining confirmed Gli1 protein and α-adrenergic receptor expression in ureteral smooth muscle and epithelial tissue with EPO receptor expression confined to the epithelial layer.Conclusions: Distal ureteral contractile forces are decreased by inhibition of Gli family proteins and the α-adrenergic receptor. EPO acts within five minutes, suggesting ion channel involvement instead of changes in gene expression. Continuing work will elucidate the role of these proteins in coordinating ureteral contractions. This has implications for the use of pharmacologic methods to address ureteral contractility and dysfunctional peristalsis during stone passage, ureteroscopy, in transplant patients and potentially to reduce symptoms from ureteral stents.

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Section: Original Articlementioning

confidence: 91%

Mediators of human ureteral smooth muscle contraction—a role for erythropoietin, tamsulosin and Gli effectors

et al. 2021

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“…A ureteral stent is the most widely used implant in urology, whose side effects in the patient's body are related to prostaglandin E 2 (PGE 2 ), a metabolite of inflammatory nephropathy caused by COX-2. SPE has been employed for the pre-concentration of PGE 2 in urine followed by analysis with the CE-MS [72]. As is shown in Figure 9, through the analysis of blank urine and five different pig urine samples, it is found that PGE 2 in the bracket group significantly increased.…”

Section: Ionic and Organic Acid-base Compoundsmentioning

confidence: 99%

Applications of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis (2019–2021)

Wang

Gong

Liu

et al. 2022

J of Separation Science

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So far, the potential of capillary electrophoresis in the application fields has been increasingly excavated due to the advantages of simple operation, short analysis time, high‐resolution, less sample consumption, and low cost. This review examines the implementations and advancements of capillary electrophoresis in different application fields (environmental, pharmaceutical, clinical, and food analysis) covering the literature from 2019 to 2021. In addition, ultrasmall sample injection volume (nanoliter range) and short optical path lead to relatively low concentration sensitivity of the most frequently used ultraviolet‐absorption spectrophotometric detection, so the pretreatment technology being developed has been gradually utilized to overcome this problem. Despite the review being focused on the development of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis, the new sample pretreatment techniques of microextraction and enrichment fit excellently to capillary electrophoresis in recent three years are also described briefly.

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“…Although these implants are commonly used, their placement can, in some cases, cause side effects, including inflammation. Huang et al [103] studied the expression of cyclooxygenase-2 and the production of its metabolite urinary prostaglandin (PGE 2 ) associated with urinary inflammatory diseases in the urine samples of pigs using the nonaqueous CE-MS method (Figure 5). The SPME was used for sample cleanup and preconcentration.…”

Section: Ce-ms For the Determination Of Diseases Of The Excretory Systemmentioning

confidence: 99%

“…The simplified diagrams of (A) the hydrodynamic sample injection, (B) the separation and stacking of analytes under the influence of the electric field, and (C) the flow-through microvial interface. Adopted from reference [103]. With permission from Elsevier mass spectrometer for the zone identification.…”

Section: F I G U R E 4 Study Design and Urinary Ce-ms Analysis Of Patients With Renal Cyst And Diabetes Syndrome (Rcad) (A)mentioning

confidence: 99%

Current applications of capillary electrophoresis‐mass spectrometry for the analysis of biologically important analytes in urine (2017 to mid‐2021): A review

Helena

Voráčová

Řemínek

et al. 2021

J of Separation Science

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Capillary electrophoresis coupled online with mass detection is a modern tool for analyzing wide ranges of compounds in complex samples, including urine. Capillary electrophoresis with mass spectrometry allows the separation and identification of various analytes spanning from small ions to high molecular weight protein complexes. Similarly to the much more common liquid chromatographymass spectrometry techniques, the capillary electrophoresis separation reduces the complexity of the mixture of analytes entering the mass spectrometer resulting in reduced ion suppression and a more straightforward interpretation of the mass spectrometry data. This review summarizes capillary electrophoresis with mass spectrometry studies published between the years 2017 and 2021, aiming at the determination of various compounds excreted in urine. The properties of the urine, including its diagnostical and analytical features and chemical composition, are also discussed including general protocols for the urine sample preparation. The mechanism of the electrophoretic separation and the instrumentation for capillary electrophoresis with mass spectrometry coupling is also included. This review shows the potential of the capillary electrophoresis with mass spectrometry technique for the analyses of different kinds of analytes in a complex biological matrix. The discussed applications are divided into two main groups (capillary electrophoresis with mass spectrometry for the determination of drugs and drugs of abuse in urine and capillary electrophoresis with mass spectrometry for the studies of urinary metabolome).

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Determination of urinary prostaglandin E2 as a potential biomarker of ureteral stent associated inflammation

Cited by 13 publications

References 59 publications

Mediators of human ureteral smooth muscle contraction—a role for erythropoietin, tamsulosin and Gli effectors

Mediators of human ureteral smooth muscle contraction—a role for erythropoietin, tamsulosin and Gli effectors

Applications of capillary electrophoresis in the fields of environmental, pharmaceutical, clinical, and food analysis (2019–2021)

Current applications of capillary electrophoresis‐mass spectrometry for the analysis of biologically important analytes in urine (2017 to mid‐2021): A review

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