Determination of vigabatrin in human plasma and urine by high-performance liquid chromatography with UV-Vis detection

Chiral chromatographic resolution of antiepileptic drugs and their metabolites: a challenge from the optimization to the application

“…Moreover, vigabatrin has no suitable chromophore to be detected by UV and no inherent fluorescence properties, requiring, therefore, derivatization techniques. Several compounds have been used as labeling agents for detection of vigabatrin by fluorescence detection (FLD) or UV detection: phenyl isothiocyanate, dansyl chloride, o ‐phthaldialdehyde (OPA), 2,4,6‐trinitrobenzene sulfonic acid (TNBSA), naproxen acyl chloride (NAC), 4‐chloro‐7‐nitrobenzofurazan, 1,2‐naphthoquinone‐4‐sulfonic acid sodium salt and 9‐flurenylmethylchloroformate (FMOC; Cetim and Atmaca, ; Amini et al ., ). However, only OPA, NAC and TNBSA have been used for the resolution of vigabatrin enantiomers by HPLC.…”

Capillary electrophoresis enantioselective separation of vigabatrin enantiomers by precolumn derivatization with dehydroabietylisothiocyante and UV–vis detection

“…The recoveries of vigabatrin enantiomers are in the range of 96.7-108%. According to the report in literature [3,6], for an adult epileptic patient, the concentrations of vigabatrin enantiomers in serum are in the range of 2-18 g/ml within 0-6 h after intake of 1000 mg RS-vigabatrin. For a healthy volunteer, the concentrations of vigabatrin in plasma are in the range of 1-24 g/ml within 0.5-24 h after a single 1000 mg vigabatrin oral dose.…”

“…In the past decade, numerous analytical methods, such as high-performance liquid chromatography (HPLC) method [4][5][6] * Corresponding authors. Tel.…”

Simultaneous HPLC-F analysis of three recent antiepileptic drugs in human plasma

“…For this reason, an original HPLC-F method, based on pre-column derivatisation with dansyl chloride (DC), has been developed for the analysis of GBP, VGB and TPR in patients' plasma. Several papers can be found in the literature for the analysis of GBP [9][10][11][12], VGB [13][14][15] or TPR [16,17]; a few other methods analyse GBP and VGB together [18][19][20][21], but none of them carries out the simultaneous analysis of all three compounds. Most methods include a derivatisation procedure to reach the desired sensitivity, e.g.…”