Determination of vitamins A, D, E, and K in human and bovine serum, and β-carotene and vitamin A palmitate in cosmetic and pharmaceutical products, by isocratic HPLC

Wang, Lai-Hao; Huang, Shin-Hung

doi:10.1007/bf02491661

Cited by 23 publications

(10 citation statements)

References 28 publications

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“…The extraction of vitamins A, D and E was performed according to the methodology described by WANG and HUANG (42) . The separation and quantification of the serum fat-soluble vitamins were carried out by high performance liquid chromatography (HPLC).…”

Section: Determination Of Serum Levels Of Fat-soluble Vitaminsmentioning

confidence: 99%

Nutritional status of patients with biliary atresia and autoimmune hepatitis related to serum levels of vitamins A, D and E

Saron

Godoy

Hessel

2009

Arq. Gastroenterol.

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-Context -Chronic liver disease may induce to malabsorption of lipids and fat-soluble vitamins, leading to injury of nutritional status. Objectives -To evaluate the nutritional status of pediatric-age patients with autoimmune hepatitis and biliary atresia related to serum levels of vitamins A, D and E and the disease severity. Methods -This controlled transverse study, evaluated the patients with autoimmune hepatitis and biliary atresia and a reference group paired by sex and age. The patients underwent anthropometric evaluation, alimentary inquiry and determination of serum levels of vitamins A, D and E by high performance liquid chromatography. The Mann-Whitney test, Spearman correlation coefficients and variance analysis (ANOVA) were utilized for data treatment, regarding significant difference if P<0.05. Results -The highest nutritional deficit was observed in patients with biliary atresia, mainly with cholestasis. The serum levels of vitamins A and E for the reference group changed as a function of age. The serum levels of vitamins A, D and E were higher in reference group than in patients with biliary atresia and autoimmune hepatitis together or separately. There were not difference in the serum levels of vitamins A, D and E between biliary atresia groups with cholestasis and without cholestasis. It was verified correlation between weight/age, triceps skinfold thickness, subscapular skinfold thickness, midarm circumference, midarm fat area values and vitamin A serum levels, as well as between all anthropometric indicators and vitamin E in patients with autoimmune hepatitis and biliary atresia. Conclusion -The patients with biliary atresia and cholestasis presented the highest nutritional injury. The patients with biliary atresia and autoimmune hepatitis presented lower serum levels of vitamins A, D and E that in control group. There is a directly proportional correlation between vitamin serum levels, mainly vitamin E, and all anthropometric variables of biliary atresia and autoimmune hepatitis groups.

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Section: Determination Of Serum Levels Of Fat-soluble Vitaminsmentioning

confidence: 99%

Nutritional status of patients with biliary atresia and autoimmune hepatitis related to serum levels of vitamins A, D and E

Saron

Godoy

Hessel

2009

Arq. Gastroenterol.

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“…The ranges of linearity for vitamins B 5 and B 6 were up to 200 μM, B 2 , B 3 , B 9 , 25(OH)D 3 , D 3 , E, and K 1 were up to 100 μM, B 1 , and A were up to 50 μM and B 7 up to 10 μM. These values indicate sufficiently high reliability that is consistent with other published techniques …”

Section: Resultsmentioning

confidence: 99%

“…For example, HPLC methods exist for a subset of water‐soluble vitamins in blood serum, multivitamin tablets, and food . HPLC assays for several fat‐soluble vitamins in blood serum, tablets, and daily products have been reported as well . There are also methods for simultaneous extraction of water‐soluble and fat‐soluble vitamins except they eventually used separate analytical methods for analysis .…”

Section: Introductionmentioning

confidence: 99%

Detection and quantification of vitamins in microliter volumes of biological samples by LC‐MS for clinical screening

et al. 2018

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A method for simultaneous determination of water‐soluble vitamins B1, B2, B3 (nicotinamide), B5, B6 (pyridoxamine), B9 and fat‐soluble vitamins E (α‐tocopherol) and K1 in tears, and B1, B2, B3, B5, B6, B9, A (retinol), and E in blood serum is described. Liquid chromatography‐mass spectrometry (LC‐MS) was used with a ternary mobile phase of water and acetonitrile containing 0.1% formic acid and methanol containing 5 mM ammonium formate. Vitamins were quantified using an internal standard method. Using 25 μL injection volumes, the limits of detection were in the range of 0.066‐5.3 ng in tear, and 0.087‐1.1 ng in serum with linear responses for all vitamins. Intra‐ and inter‐day precision and recoveries were satisfactory. This is the first study to demonstrate simultaneous vitamin detections in microliters of biological samples which has distinct advantages in many diagnostic applications with limited available fluids (e.g., tears; elderly anemic blood) or sampling small subjects (e.g., rodents). © 2018 American Institute of Chemical Engineers AIChE J, 64: 3709–3718, 2018

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“…Menaquinone-4 (MK-4) was analysed according to the method of Wang and Huang (2002). Menaquinone-7 (MK-7) analysis was performed by HPLC (Shimadzu, Japan) using a 250 mm×4.6 mm ID Lichrosper® 100 C18 column containing 5 mm sized particles and a 20 ml loop injector.…”

Section: Determination Of Vitamin K (Mk-7 and Mk-4 Content)mentioning

confidence: 99%

Effect of sequential bio-processing conditions on the content and composition of vitamin K2 and isoflavones in fermented soy food

2015

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In the present research, effect of sequential addition of Bifidobacterium bifidum, Bacillus subtilis and Rhizopus oligosporus on content and composition of vitamin K 2 and isoflavones in fermented soy foods have been investigated. Initially, soybeans were fermented with B. bifidum; then this fermented mass was re-fermented with co-culture of B. subtilis and R. oligosporus. The evolved sequence of microbes inoculation tended towards significantly (p <0.5) higher enzymes levels (126.16±2.23 IU/mg lipase, 36.52± 1.25 IU/mg phytase and 8.52±1.12 IU/mg β-glucosidase); maximum menaquinone-7 production (9.3±1.27 μg/g); and isoflavone content (84.64±1.97 % daidzein, 99.29±0.86 % genistein, 96.42±1.32 % glycitein) after 72 h of solid-state fermentation. The study showed that co-fermentation of soybean with different microbes in a particular sequence can enhance nutritional value batter than the mono-culture fermentation due to the positive correlation between enzymes (lipase, phytase, β-glucosidase) levels, menaquinone-7 and soy isoflavones content.

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Determination of vitamins A, D, E, and K in human and bovine serum, and β-carotene and vitamin A palmitate in cosmetic and pharmaceutical products, by isocratic HPLC

Cited by 23 publications

References 28 publications

Nutritional status of patients with biliary atresia and autoimmune hepatitis related to serum levels of vitamins A, D and E

Nutritional status of patients with biliary atresia and autoimmune hepatitis related to serum levels of vitamins A, D and E

Detection and quantification of vitamins in microliter volumes of biological samples by LC‐MS for clinical screening

Effect of sequential bio-processing conditions on the content and composition of vitamin K2 and isoflavones in fermented soy food

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