Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay (ELISA)

Battelli, Maria Giulia; Abbondanza, Ada; Musiani, S; Buonamici, Laura; Strocchi, Paola; Tazzari, Pier Luigi; Gramantieri, Laura; Stirpe, Fiorenzo

doi:10.1016/s0009-8981(98)00220-4

Cited by 26 publications

(12 citation statements)

References 37 publications

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“…Conversion of HX to X to UA by xanthine oxidase, and structure of uridine (I.S.). [22], fluorometric [23,24] and ELISA [25] methods in serum. The serum/plasma activities were reported to be very low.…”

Section: Introductionmentioning

confidence: 99%

Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies

Cooper¹,

Khosravan²,

Erdmann³

et al. 2006

Journal of Chromatography B

175

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Section: Introductionmentioning

confidence: 99%

Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies

Cooper¹,

Khosravan²,

Erdmann³

et al. 2006

Journal of Chromatography B

175

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“…To date, the activity of xanthine oxidase has been determined by HPLC method for measuring the conversion of pterin to isoxanthopterin in human plasma [11][12][13][14][15], radiometric [16], fluorometric [17,18] and ELISA [19] methods in serum. The serum/plasma activities were reported to be very low.…”

Section: Discussionmentioning

confidence: 99%

A Sensitive Reversed-Phase High-Performance Liquid Chromatography Method for the Quantitative Determination of Milk Xanthine Oxidase Activity

Li¹,

Guan²,

Liu³

2013

OJMC

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A new reversed-phase high performance liquid chromatography method was developed to quantitate the activity of xanthine oxidase involved in milk fat globule membrane with xanthine as the substrate and the separation of product (uric acid). The increment of uric acid in the reaction system was used to calculate the total activity of XO. The optimized assay conditions, linearity of detection, recovery of uric acid and chromatogram were developed in text, indicating this method is simple, rapid and efficient. It is an alternative potential method for the determination of the activity of XO in milk.

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“…The uric acid levels of the group treated with OA increased 1.5 times after 2 weeks after the intraperitoneal injection and increased 2.5 times after 5 weeks, compared with the control group. The enzyme XO exists in most mammalian tissues, including rat plasma, with its highest concentrations in the liver and the intestine (Battelli et al, 1999). Uric acid is generated when purine catabolism of hypoxanthine and xanthine is catalyzed by XO.…”

Section: Discussionmentioning

confidence: 99%

Hibiscus sabdariffa L. extracts reduce serum uric acid levels in oxonate-induced rats

Kuo

Kao

Chan

et al. 2012

Journal of Functional Foods

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Hibiscus sabdariffa extractOxonic acid Uricase Xanthine oxidase A B S T R A C T Elevated serum levels of uric acid are associated with an increased risk for hyperuricemia, gout, hypertension, cardiovascular disease, and renal failure. Recent attention has focused on the bioactive properties of edible plants in preventing disease. Hibiscus sabdariffa L., a local soft drink material and medicinal herb in Taiwan, is used effectively in native medicines against hypertension, pyrexia, and liver disorders. We investigated the effects of the Hibiscus sabdariffa extract (HSE) on oxonic acid (OA)-induced hyperuricemia in rats. The HSE affected serum uric acid levels and urate enzymes such as uricase and xanthine oxidase (XO). We treated rats intraperitoneally with normal saline and oxonate solution for 1 week and with or without feeding allopurinol (an XO inhibitor) or HSE (1%, 2%, and 5%) for 5 weeks. We observed that treatment with HSE inhibited OA-induced hyperuricemia, with a greater uric acid lowering effect than allopurinol treatment. Our results showed that HSE effectively inhibited OA-induced hyperuricemia by decreasing uric acid and increasing uricase activity, but not by affecting XO activity.

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Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay (ELISA)

Cited by 26 publications

References 37 publications

Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies

Quantification of uric acid, xanthine and hypoxanthine in human serum by HPLC for pharmacodynamic studies

A Sensitive Reversed-Phase High-Performance Liquid Chromatography Method for the Quantitative Determination of Milk Xanthine Oxidase Activity

Hibiscus sabdariffa L. extracts reduce serum uric acid levels in oxonate-induced rats

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