Determination of Zearalenone in Barley, Maize and Wheat Flour, Polenta, and Maize-Based Baby Food by Immunoaffinity Column Cleanup with Liquid Chromatography: Interlaboratory Study

MacDonald, Susan; Anderson, Sharron; Brereton, Paul; Wood, Roger; Damant, Andrew; Aletrari, Maria; Garcı́a-Alonso, Susana; Burdaspal, P. A.; Darroch, J; Donnelly, Carol; Durand, T; Felguerias, I; French, R. B.; Griffin, Jasper; Heide, C. von der; Herry, Marie P; Hollywood, F.; Howe, Alan M.; Ioannou–Kakouri, Eleni; Johnson, T; Kernaghan, I; Krska, Rudolf; Nisbet, J A; Pettersson, Hans; Procter, Jo L.; Rawcliffe, Peter; Smith, Anna Jo Bodurtha; Smith, William P.; Stangroom, S; Stevens, Charles L.; Swanson, William J.; Sweet, P.; Thomas, Michael B.; Waller, J. G.; Welsh, Paul

doi:10.1093/jaoac/88.6.1733

Cited by 61 publications

(21 citation statements)

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“…Standards of AFs (in toluene:ACN, 9:1 v/v), OTA (in toluene:acetic acid, 99:1 v/v), ZON, DON, T-2 and HT-2 (in ACN), FB 1 and FB 2 (in ACN:H 2 O, 50:50 v/v) were prepared by dissolving the solid pure substance in the appropriate solvent and were stored at −18°C until use. The concentration of the stock solution was measured by UV spectroscopy (Shimadzu, UV-1700) according to AOAC Official methods (AOAC, 2010a(AOAC, , 2010b(AOAC, , 2010cMacDonald, Anderson, et al, 2005;MacDonald, Chan, et al, 2005). Working standard solutions of known concentrations were prepared by the appropriate dilution of the stock solution.…”

Section: Preparation Of Standard Solutionsmentioning

confidence: 99%

“…Several analytical (chemical and biochemical) methods have been developed for the determination of individual mycotoxins after immunoaffinity column clean-up (AOAC, 2010a(AOAC, , 2010b(AOAC, , 2010c(AOAC, , 2010dCano-Sancho, Sanchis, Marin, & Ramos, 2013;Li et al, 2014;Majeed, Iqbal, Rafique, & Zafar, 2013;MacDonald, Anderson, Brereton, Wood, & Damant, 2005;MacDonald, Chan, Brereton, Damant, & Wood, 2005;Rahmani, Jinap, & Soleimany, 2009;Rahmani, Jinap, Soleimany, Khatib, & Tan, 2011;Stroka, Anklam, Jorissen, & Gilbert, 2000;Vaclavikova, MacMahon, Zhang, & Begley, 2013). Chromatographic methods commonly used for the quantitative determination of mycotoxins in foodstuffs include thin-layer chromatography (TLC) (Stroka, Otterdijk, & Anklam, 2000), high-performance liquid chromatography (HPLC) coupled with ultraviolet (UV), photo diode array, fluorescence detectors or mass spectrometry (MS), and gas chromatography coupled with electron capture, flame ionization or MS detectors (Krska et al, 2008;Shephard et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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A validated UPLC-MS/MS multi-mycotoxin method for nuts and cereals: results of the official control in Cyprus within the EU requirements

Kafouris

Christofidou

Christodoulou

et al. 2016

Food and Agricultural Immunology

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The validation of a rapid, reliable and sensitive method for the simultaneous determination of Aflatoxins, Ochratoxin A, Zearalenone, Deoxynivalenol, Fumonisins, T-2 and HT-2 toxins, in nuts and cereals is reported. This method is based on a single extraction step using an acetonitrile/water mixture followed by the analysis of the diluted crude extract using ultra highperformance liquid chromatography with tandem mass spectrometry. The method performance characteristics were determined after spiking blank samples. The mean recoveries in spiked nuts ranged from 74.4% to 131.7%, while in cereals ranged from 52.8% to 113.9%. Limits of detection and quantification for nuts and cereals ranged 0.08-30.0 and 0.25-99.0 μg/kg, respectively. Validated method was used for monitoring mycotoxins in 120 samples. A total of 97 samples (81%) were contaminated with at least one of these mycotoxins, but only the 3% of the samples exceeded the maximum levels of the EU legislation.

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Section: Preparation Of Standard Solutionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A validated UPLC-MS/MS multi-mycotoxin method for nuts and cereals: results of the official control in Cyprus within the EU requirements

Kafouris

Christofidou

Christodoulou

et al. 2016

Food and Agricultural Immunology

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“…ZEA analysis was performed according to the procedure of MacDonald et al (2005a) with slight modifications. In particular, 20 g of ground sample was extracted with 80 ml of acetonitrile–water (75:25, v/v), by blending at high speed for 2 min.…”

Section: Methodsmentioning

confidence: 99%

Stability of Fusarium toxins during traditional Turkish maize bread production

Numanoğlu¹,

Uygun²,

Köksel³

et al. 2010

Quality Assurance and Safety of Crops &Amp; Foods

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Introduction The occurrence of major Fusarium toxins in maize samples collected in the Black Sea region of Turkey and fate of these toxins during traditional Turkish maize bread production was investigated. Materials and methods Twenty maize samples were analysed for deoxynivalenol, zearalenone, fumonisins, T-2 and HT-2 toxins. Contamination profiles of maize samples were determined in order to choose the toxins to be monitored during bread production. Breads were produced from low or uncontaminated maize samples and from the mixture of highly contaminated ones. After baking, crust and crumb of the breads were separated and analysed by high-performance liquid chromatography standard methods with ultraviolet or fluorescence detection. Results and conclusions In total, toxin levels in 30% of the maize samples were found to be higher than the limits established by the European Union. After bread processing no significant reduction of deoxynivalenol, zearalenone and fumonisins was measured in the bread crust and crumb. According to the mass balance of mycotoxins measured in maize flour and relevant maize bread only 2.1%, 0.1% and 3.1% of the total initial amounts of deoxynivalenol, zearalenone and fumonisins, respectively, were lost. Breads were not analysed for T-2 and HT-2 as their levels in maize were quite low ( o 50 mg kg À1 ).

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“…It exhibits blue‐green fluorescence when excited by long wavelength UV light (360 nm) and a more intense green fluorescence when excited with short wavelength UV light (260 nm; Yu, Wang, & Sun, ). Other techniques like HPLC/IAC, atmospheric pressure chemical ionization (APCI) or electrospray ionization interface and LC‐MS/MS have been commonly used for the measurement of zearalenone (ZEA; Berthiller, Schuhmacher, Buttinger, & Krska, ; Macdonald et al, ).…”

Section: Introductionmentioning

confidence: 99%

Rapid and sensitive detection of mycotoxins by advanced and emerging analytical methods: A review

Singh

Mehta

2020

Food Science & Nutrition

150

108

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Quantification of mycotoxins in foodstuffs is extremely difficult as a limited amount of toxins are known to be presented in the food samples. Mycotoxins are secondary toxic metabolites, made primarily by fungal species, contaminating feeds and foods. Due to the presence in globally used grains, it is an unpreventable problem that causes various acute and chronic impacts on human and animal health. Over the previous few years, however, progress has been made in mycotoxin analysis studies. Easier techniques of sample cleanup and advanced chromatographic approaches have been developed, primarily high-performance liquid chromatography. Few extremely sophisticated and adaptable tools such as high-resolution mass spectrometry and gas chromatography-tandem MS/MS have become more important. In addition, Immunoassay, Advanced quantitative techniques are now globally accepted for mycotoxin analysis. Thus, this review summarizes these traditional and highly advance methods and their characteristics for evaluating mycotoxins. K E Y W O R D Sadvanced quantitative techniques, chromatography, immunological, mycotoxins, spectroscopy 2184 | SINGH aNd MEHTa ultraviolet, fluorescence, photomultiplier, ion mobility, and tandem mass spectrophotometry, fourier transforms near infrared, adsorptive stripping voltammetry, and their lower detection limits, and sensitivity of different types of matrice has been reviewed. For the determination of mycotoxins, traditional quantitative methods viz. chromatography, immunological, and the advanced methods viz. ultrahigh-performance liquid chromatography, fluorescence polarization immunoassay, nanoparticle-based methods, microfluidics, and phage display methods have been discussed extensively in this review.

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Determination of Zearalenone in Barley, Maize and Wheat Flour, Polenta, and Maize-Based Baby Food by Immunoaffinity Column Cleanup with Liquid Chromatography: Interlaboratory Study

Cited by 61 publications

References 0 publications

A validated UPLC-MS/MS multi-mycotoxin method for nuts and cereals: results of the official control in Cyprus within the EU requirements

A validated UPLC-MS/MS multi-mycotoxin method for nuts and cereals: results of the official control in Cyprus within the EU requirements

Stability of Fusarium toxins during traditional Turkish maize bread production

Rapid and sensitive detection of mycotoxins by advanced and emerging analytical methods: A review

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