2000
DOI: 10.1002/(sici)1096-9888(200001)35:1<71::aid-jms915>3.0.co;2-5
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Determination ofin vitro permeability of drug candidates through a Caco-2 cell monolayer by liquid chromatography/tandem mass spectrometry

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Cited by 96 publications
(58 citation statements)
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“…Caco-2 cell monolayers with TEER values greater than 500 Ω cm 2 were considered for experimentation. Digoxin (5 µM) was used as a positive control for P-gp substrate [10]. The concentration of ulixertinib used in the assay was 10 µM.…”
Section: Caco-2 Permeabilitymentioning
confidence: 99%
“…Caco-2 cell monolayers with TEER values greater than 500 Ω cm 2 were considered for experimentation. Digoxin (5 µM) was used as a positive control for P-gp substrate [10]. The concentration of ulixertinib used in the assay was 10 µM.…”
Section: Caco-2 Permeabilitymentioning
confidence: 99%
“…For the analysis of samples from in vitro permeability tests [8][9][10][11][12], high performance liquid chromatography (HPLC) coupled with mass spectrometric detection has been widely used. LC/MS/MS offers the advantages of increased sensitivity and specificity [13].…”
Section: Introductionmentioning
confidence: 99%
“…The trapped analytes are back-eluted from the on-line trap column and further chromatographically resolved on a second analytical C18 column. Combining the TFC technique with the specificity of a mass spectrometer operating in the selected reaction monitoring mass spectrometry (SRM) mode permits Caco-2 cell analysis in a cassette N-in-1 mode [21][22][23][24]. Several different researchers have published papers for utilizing cassette mode approach.…”
Section: Introductionmentioning
confidence: 99%