Determination the site of antibiotic resistance genes in Escherichia coli isolated From Urinary Tract Infection

Abstract

This study includes isolation of 25 isolates of Escherichia coli (E. coli ) strain from urinary tract samples in a pregnant woman. Microbiological and biochemical tests were used to identify the resistant bacteria of this genus. Screening methods were used to determine bacterial isolates for their resistance to 10 antibiotics include: Amikacin (Ak), Amoxicillin (Ax), Ampicillin (Ap), Chloramphenicol (Cm), Ciprofloxacin (Cip), Erythromycin (Er), Nalidixic acid (Nal), Penicillin (Pen), Tetracycline (Tet) and Trimethoprim (Tm). The isolates E4, E9, E16, and E17 were resistant to all antibiotics used in the current study using the disk diffusion method. In contrast, the resistance percentage for all antibiotics ranged between 28-96%. Sites of resistance genes and hemolysin production genes were characterized by tranformation techniques in the E4 and E16. The results showed that the antibiotic resistance genes of Amikacin, Erythromycin, Tetracyclin, and Trimethoprim were located on a plasmid, whereas Amoxicillin, Ampicillin, Chloramphenicol, Ciprofloxacin, Nalidixic acid and Penicillin were located on chromosomal DNA. The results also demonstrated an inability to produce alpha or beta-hemolysin indicating that the genes which are responsible for hemolysin production were also located on chromosomal DNA.

Phenotypic and Molecular Correlation between Biofilm Production and Antibiotic Resistance Escherichia coli that Isolated from Heart Catheterization Patients

“…This study found that tetracycline and chloramphenicol resistance was each at 46.6%, whereas (Mohammed, 2018) in Iraq found that the same antibiotics were each resistant to 88%, and (Abdel Hamid and Abozahra, 2017) in Egypt found that tetracycline resistance was 64.3% by E. coli. This might be because the bacteria altered the permeability of its outer membrane and had efflux pumps.…”

Evaluation of some Virulence Factors and Drug Resistance of Bacteria Isolated from the Urine of Patients with TCC-Bladder Cancer

“…While (100%) were sensitive to imipenem and meropenem 19 . Another study in Iraq in Erbil 2018 included 25 isolates of UPEC, resistance percentage between 28 to 96 % to amikacin, amoxicillin, ampicillin, chloramphenicol, ciprofloxacin, erythromycin, nalidixic acid, penicillin, tetracycline, and trimethoprim 20 . A study in Iran in 2018 included 60 strains of uropathicE.coli was investigated antibiotic susceptibility using the Kirby Bauer disk diffusion method.…”