In this research, it is aimed to determine pre-service science teachers' environment attitude, behavior and perceptions in terms of different variables. In this research, the relational screening model method was used. The study group consists of 265 preservice science teachers from a state university in Turkey. The research was conducted during the fall semester of 2015-2016. In this research, the Environment Attitude Scale (EAS), Environment Behavior Scale (EBS), and Environment Perceptions Scale (EPS) were used. Data were analyzed by using IBM SPSS-21 statistical program. For data analysis, Independent t-test, Mann-Whitney U-test, One-Way Variance Analysis (ANOVA), Tukey significance test and also Correlation Analysis were used. There was no statistically significant difference found in pre-service teachers' environment attitude, behavior and perceptions in terms of gender and longest-lived place. However, there was a statistically significant difference found in pre-service teachers' environment attitude, behavior and perceptions in terms of the variables of having an environment lesson, education department and grade level. There was a positive middle relationship between environmental literacy components and environment attitude behavior, and a positive weak relationship between environment attitudeperceptions.