Determining the antifungal activity and characterization of Bacillus siamensis AMU03 against Macrophomina phaseolina (Tassi) Goid

Hussain, Touseef; Khan, Abrar Ahmad

doi:10.1007/s42360-020-00239-6

Cited by 28 publications

(18 citation statements)

References 31 publications

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“…methods, gel retardation experiments, and electron microscopy (Zhang et al, 2013). Previously, B. siamensis strain isolation has been reported from rhizosphere or other sources other than endophytic (Hussain and Khan, 2020;Yoo et al, 2020;Pastor-Bueis et al, 2017;Islam et al, 2019). Recently, Putri et al, 2020, showed antifungal activity of ethyl acetate extract obtained from fermentation filtrate of B. siamensis against Aspergillus niger.…”

Section: Discussionmentioning

confidence: 99%

Screening of Tomato Seed Bacterial Endophytes for Antifungal Activity Reveals Lipopeptide Producing Bacillus siamensis Strain NKIT9 as a Potential Bio-Control Agent

et al. 2021

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The current study investigates the diversity pattern and fungicidal potential of bacterial endophytes isolated from two different organic varieties of tomato plants (V1 and V2). A total of seventy-five bacterial isolates identified by 16S rRNA gene sequencing revealed a majority of genus as Bacillus and one Planococcus, which were grouped into eight different species. The Shannon diversity H’ (1.56), Simpson’s index of diversity (0.93), Magalef’ index (2.23), Evenness (0.96), and Species richness (7) indicated the high endophytic bacterial diversity in the V1 variety of the tomato. Bacterial endophytes isolated from both of the varieties were screened for their antifungal activity against five economically critical fungal pathogens (viz., Botrytis cinerea, Rhizoctonia solani, Fusarium solani, Verticillium lateritium, and Alternaria solani) of tomato crop through dual culture assay. The data revealed B. siamensis strain NKIT9 as the most potent antagonist, significantly (p < 0.05) inhibiting the mycelial growth between 75 to 90% against selected fungal pathogens. High bioactivity of lipopeptide extract of strain NKIT9 was recorded against R. solani with minimum IC50 value of 230 μg/ml. The Ultra Performance Liquid Chromatography-High Definition Mass Spectrometry (UPLC-HDMS) analysis of this lipopeptide extract revealed the presence of Surfactin and Bacillomycin D. Furthermore, in-vitro results showed that the selected bacterial strain significantly minimized the disease incidence in damping-off assay which makes this strain a promising antifungal bio-control agent. Moreover, in the pot experiment the NKIT9 increased the fruit yield by 59.2% compared with the untreated R. solani infested control.

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Section: Discussionmentioning

confidence: 99%

Screening of Tomato Seed Bacterial Endophytes for Antifungal Activity Reveals Lipopeptide Producing Bacillus siamensis Strain NKIT9 as a Potential Bio-Control Agent

et al. 2021

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“…Several isolates were quite effective in reducing disease incidence and promoting host plant growth traits. Among these are species belonging to Bacillus, Pantoea, Pseudomonas, Stenotrophomonas, and Serratia genus (Kumar et al, 2007;Vasebi et al, 2013;Torres et al, 2016;Mmbaga et al, 2018;Sanjeevkumar et al, 2020;Hussain and Khan, 2020).…”

Section: Bacterial Bcasmentioning

confidence: 99%

“…Bacillus amyloliquefaciens and B. siamensis have shown antifungal activities via the excretion of compounds of the lipopeptides-surfactin class, although further studies are required to understand the exact composition and molecular structure of the filtrates. For instance, a lethal damage on the fungus microsclerotia was observed (Torres et al, 2016;Hussain and Khan, 2020). Meanwhile, B. subtilis generated a fungistatic effect probably connected to a competition for space or nutrients, instead of a toxic effect (Torres et al, 2016).…”

Section: Bacterial Bcasmentioning

confidence: 99%

Macrophomina phaseolina: General Characteristics of Pathogenicity and Methods of Control

et al. 2021

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Macrophomina phaseolina is a generalist soil-borne fungus present all over the world. It cause diseases such as stem and root rot, charcoal rot and seedling blight. Under high temperatures and low soil moisture, this fungus can cause substantial yield losses in crops such as soybean, sorghum and groundnut. The wide host range and high persistence of M. phaseolina in soil as microsclerotia make disease control challenging. Therefore, understanding the basis of the pathogenicity mechanisms as well as its interactions with host plants is crucial for controlling the pathogen. In this work, we aim to describe the general characteristics and pathogenicity mechanisms of M. phaseolina, as well as the hosts defense response. We also review the current methods and most promising forecoming ones to reach a responsible control of the pathogen, with minimal impacts to the environment and natural resources.

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“…The majority of B. siamensis strain isolation has been reported from rhizosphere or other sources other than endophytic (Yoo et al, 2020; Hussain and Khan. 2020; Islam et al, 2019; Pastor-Bueis et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Screening of tomato seed bacterial endophytes for antifungal activity reveals lipopeptide producing Bacillus siamensis as a potential bio-control agent

Sharma

Kaushik

Sharma

et al. 2020

Preprint

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The current study investigates the diversity pattern and fungicidal potential of bacterial endophytes isolated from two different organic varieties of tomato plants (V1 and V2). A total of seventy-four bacterial isolates identified by 16S rRNA sequencing revealed a single genus Bacillus with 16 different species. The Shannon diversity H’ (1.45), Simpson’s index of diversity (0.9), Magalef’ index (2.1), Evenness (0.96), and Species richness (8) indicated the high endophytic bacterial diversity in the V1 variety of the tomato. Bacterial endophytes isolated from both the varieties were screened for their antifungal activity against five economically critical fungal pathogens (viz., Botrytis cinerea, Rhizoctonia solani, Fusarium solani, Verticillium lateritium, and Alternaria solani) of tomato crop through dual culture assay. The data revealed B. siamensis KCTC 13613(T) as the most potent antagonist significantly (p < 0.05), inhibiting the mycelial growth between 75 to 90% against selected fungal pathogens. High bioactivity of lipopeptide extract of B. siamensis was recorded against R. solani with IC50 value of 72 ppm. The UPLC-HDMS analysis of this lipopeptide extract revealed the presence of, Surfactin and Bacillomycin D.

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Determining the antifungal activity and characterization of Bacillus siamensis AMU03 against Macrophomina phaseolina (Tassi) Goid

Cited by 28 publications

References 31 publications

Screening of Tomato Seed Bacterial Endophytes for Antifungal Activity Reveals Lipopeptide Producing Bacillus siamensis Strain NKIT9 as a Potential Bio-Control Agent

Screening of Tomato Seed Bacterial Endophytes for Antifungal Activity Reveals Lipopeptide Producing Bacillus siamensis Strain NKIT9 as a Potential Bio-Control Agent

Macrophomina phaseolina: General Characteristics of Pathogenicity and Methods of Control

Screening of tomato seed bacterial endophytes for antifungal activity reveals lipopeptide producing Bacillus siamensis as a potential bio-control agent

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