Determining the effect of calcium on cell death rate and perforation formation during leaf development in the novel model system, the lace plant (<i>Aponogeton madagascariensis</i>)

Fraser, Meredith S.; Dauphinee, Adrian N.; Gunawardena, Arunika H. L. A. N.

doi:10.1111/jmi.12859

Cited by 7 publications

(5 citation statements)

References 48 publications

(96 reference statements)

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“…minor system (Kaźmierczak et al 2021 ) showed an increase of cytosolic calcium, which was accentuated by Ruthenium Red, suggesting that this calcium originates from internal stores, which is consistent with the current study. However, in the V. faba system, the elevated cytosolic calcium in presence of Ruthenium Red was accompanied by reduced cell death, which was also seen in the lace plant (Fraser et al 2020 ). Thus, a bulk increase of cytosolic calcium does not per se lead to more pronounced cell death.…”

Section: Discussionmentioning

confidence: 80%

“…The induction of cytosolic calcium during cytokinin-dependent cell death is consistent with findings in tobacco BY-2 cells, where inhibition of calcium influx by lanthanum ions mitigated cell death in response to hydrogen peroxide (Bobal et al 2015 ). Conversely, the development of leaf perforations in the lace plant, a unique system to study developmental PCD, was promoted by the calcium ionophore A23187 and inhibited by the calcium channel inhibitor Ruthenium Red (Fraser et al 2020 ). There is one important difference, though.…”

Section: Discussionmentioning

confidence: 99%

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Kinetin induces microtubular breakdown, cell cycle arrest and programmed cell death in tobacco BY-2 cells

Kaźmierczak

Siatkowska

et al. 2022

Protoplasma

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Plant cells can undergo regulated cell death in response to exogenous factors (often in a stress context), but also as regular element of development (often regulated by phytohormones). The cellular aspects of these death responses differ, which implies that the early signalling must be different. We use cytokinin-induced programmed cell death as paradigm to get insight into the role of the cytoskeleton for the regulation of developmentally induced cell death, using tobacco BY-2 cells as experimental model. We show that this PCD in response to kinetin correlates with an arrest of the cell cycle, a deregulation of DNA replication, a loss of plasma membrane integrity, a subsequent permeabilisation of the nuclear envelope, an increase of cytosolic calcium correlated with calcium depletion in the culture medium, an increase of callose deposition and the loss of microtubule and actin integrity. We discuss these findings in the context of a working model, where kinetin, mediated by calcium, causes the breakdown of the cytoskeleton, which, either by release of executing proteins or by mitotic catastrophe, will result in PCD.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Kinetin induces microtubular breakdown, cell cycle arrest and programmed cell death in tobacco BY-2 cells

Kaźmierczak

Siatkowska

et al. 2022

Protoplasma

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“…The exogenous Ca 2+ carrier A23187 can stimulate PCD in Nicotiana tobacum cell cultures (Óbrien and Ferguson, 1997). During PCD regulation of leaf perforation formation in Aponogeton madagascariensis, the exogenous Ca 2+ carrier A23187 accelerated the occurrence of PCD and thus increased the rate of perforation formation (Fraser et al, 2020). In addition, this study found that the endogenous Ca 2+ content of P. lacti ora 'Fen Yu Nu' pollen was signi cantly reduced when the appropriate concentration of Ca 2+ chelating agent EGTA was applied to the pollen after LN storage, and the activity of caspase-3-like and caspase-9-like protease, the rate of apoptosis and living cells were inhibited to a certain extent with EGTA treatment, but there was no signi cant effect on the mitochondrial membrane potential (Fig.…”

Section: Discussionmentioning

confidence: 99%

Ca2+ participates in programmed cell death by modulating ROS during pollen cryopreservation

et al. 2022

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Programmed cell death (PCD) is one of the reasons for the decline in pollen viability after cryopreservation. However, the role of calcium ions (Ca 2+ ) in PCD during pollen cryopreservation has not been revealed in the existing studies. In this study, Paeonia lacti ora 'Fen Yu Nu' pollen was used as the research material for investigating the effects of Ca 2+ changes on PCD indices and reactive oxygen species (ROS) during pollen cryopreservation. The results showed that after cryopreservation, with the decrease of pollen viability, the Ca 2+ content signi cantly increased. The regulation of Ca 2+ content had a signi cant effect on PCD indices, which showed that the Ca 2+ carrier A23187 accelerated the decrease of mitochondrial membrane potential level and increased the activity of caspase-3-like and caspase-9-like proteases and the apoptosis rate. The expression levels of partial pro-PCD genes were upregulated, the anti-PCD gene BI-1 was downregulated, and the addition of Ca 2+ chelating agent EGTA had the opposite effect. The addition of the Ca 2+ carrier A23187 after cryopreservation signi cantly increased the ROS content of pollen, the addition of the Ca 2+ chelating agent EGTA had the opposite effect, and Ca 2+ regulators also had signi cant effects on the contents of ROS production and clearance-related substances. Ca 2+ affected intracellular ROS content by acting on the ROS production and clearance system during the cryopreservation of pollen and is thus involved in the occurrence of PCD.

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“…This pigmentation disappears as leaves mature and chlorophyll pigmentation becomes more prominent (Figure 1C). The lace plant is an excellent model system for studying developmentally regulated PCD in planta due to the accessibility and predictability of perforation formation, the suitability of its thin and semi-transparent leaves for live cell imaging, and the established sterile culture system for propagation [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

In vitro Anticancer Activity of Aponogeton madagascariensis Anthocyanin Extracts

Gunawardena

Rollini

Rasmussen³

et al. 2021

J NHP Res

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INTRODUCTION:Aponogeton madagascariensis (lace plant) is a freshwater aquatic flowering plant belonging to the family Aponogetonaceae that forms leaf perforations via programmed cell death (PCD). The lace plant has emerged as a novel model system for studying PCD in planta due to the predictability and accessibility of this process. Anthocyanins, and the balance between ROS and antioxidants, play a central role in regulating PCD in lace plant leaves. Aponogetonaceae family members have shown medicinal properties, including antioxidant and anticancer activities; however, nothing is known about the lace plant's potential for medicinal use. Therefore, this study evaluated the anticancer activities of lace plant anthocyanin extracts. METHODS:Cell line growth and viability were assessed following exposure to lace plant leaf anthocyanin extracts. This study utilized a triple-negative breast cancer cell line, MDA-MB-231, two human ovarian epithelial cancer cell lines, OVCAR-8 and SKOV-3, along with a normal mammary epithelial cell line, MCF-10A. Furthermore, crude anthocyanin extracts were fractionated into anthocyanin and non-anthocyanin containing fractions and tested only on MDA-MB-231 cells. RESULTS AND DISCUSSION:The crude anthocyanin extracts from lace plant leaves inhibited the growth of MDA-MB-231, OVCAR-8, and SKOV-3 cells in a concentration-dependent manner and had no effect on MCF-10A cells. Lace plant crude anthocyanin extracts appeared to induce apoptosis in MDA-MB-231 cells. Interestingly, treatment with anthocyanin and non-anthocyanin fractions decreased the growth of MDA-MB-231, similarly to crude anthocyanin extracts, suggesting the presence of other anticancer compounds in the lace plant extracts.

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Determining the effect of calcium on cell death rate and perforation formation during leaf development in the novel model system, the lace plant (Aponogeton madagascariensis)

Cited by 7 publications

References 48 publications

Kinetin induces microtubular breakdown, cell cycle arrest and programmed cell death in tobacco BY-2 cells

Kinetin induces microtubular breakdown, cell cycle arrest and programmed cell death in tobacco BY-2 cells

Ca2+ participates in programmed cell death by modulating ROS during pollen cryopreservation

In vitro Anticancer Activity of Aponogeton madagascariensis Anthocyanin Extracts

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