Determining Vitamin D Status: A Comparison between Commercially Available Assays

Snellman, Greta; Melhus, Håkan; Gedeborg, Rolf; Byberg, Liisa; Berglund, Lars; Wernroth, Lisa; Michaëlsson, Karl

doi:10.1371/journal.pone.0011555

Cited by 195 publications

(156 citation statements)

References 33 publications

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“…However, both recommendations of vitamin D intake and adequate serum concentrations of calcidiol still remain controversial. The latter is even more hindered in view of unreliable and often inaccurate analytical methods for its detection (Snellman et al 2010).…”

Section: Calcitriol As a Mediator For All Three Tissuesmentioning

confidence: 99%

Aging human body: changes in bone, muscle and body fat with consequent changes in nutrient intake

Jafarinasabian

Inglis

Reilly

et al. 2017

Journal of Endocrinology

226

147

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Aging affects almost all physiological processes, but changes in body composition and body phenotype are most observable. In this review, we focus on these changes, including loss of bone and muscle and increase in body fat or redistribution of the latter, possibly leading to osteosarcopenic obesity syndrome. We also address low-grade chronic inflammation, prevalent in aging adults and a cause of many disorders including those associated with body composition. Changes in dietary intake and nutritional requirements of older individuals, that all may lead to some disturbances on tissue and organ levels, are discussed as well. Finally, we discuss the hormonal changes in the aging body, considering each of the tissues, bone, muscle and fat as separate endocrine organs, but yet in the continuous interface and communication with each other. Although there are still many unanswered questions in this field, this review will enable the readers to better understand the aging human body and measures needing to be implemented toward reducing impaired health and disability in older individuals.

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Section: Calcitriol As a Mediator For All Three Tissuesmentioning

confidence: 99%

Aging human body: changes in bone, muscle and body fat with consequent changes in nutrient intake

Jafarinasabian

Inglis

Reilly

et al. 2017

Journal of Endocrinology

226

147

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“…Masscheck calibration materials and controls are traceable against nISt 972 reference material. Furthermore, the lc-MS/MS method of analysis has previously been validated against other commercially available assays and is regarded as the most valid and reliable technique for the assessment of vitamin D metabolites (Snellman et al 2010).…”

Section: Analysis Of Vitamin D Metabolites and Intact Parathyroid Hormentioning

confidence: 99%

Vitamin D supplementation does not improve human skeletal muscle contractile properties in insufficient young males

et al. 2014

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12 (25 ± 15 nmol l −1 ) compared with baseline. Despite marked increases in total serum 25[OH]D in VItD and a decrease in PlB, there were no significant changes in any of the muscle function outcome measures at week 6 or 12 for either group (P > 0.05). Conclusions Elevating total serum 25[OH]D to concentrations > 120 nmol l −1 has no effect on skeletal muscle function. We postulate that skeletal muscle function is only perturbed in conditions of severe deficiency (<12.5 nmol l

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“…Effects of variable recoveries and DBP concentration changes on different patient populations can have significant impact on assay accuracy and precision (22)(23)(24)(25). Manufacturers have recognized increasing demands for vitamin D testing and are working on improving the existing kits to provide reliable and reproducible results.…”

Section: Vitamin D Assaysmentioning

confidence: 99%

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

Babić¹

2012

Journal of Medical Biochemistry

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Summary: Demand for vitamin D testing has been on a constant rise worldwide, partially due to mounting evidence linking vitamin D status to overall health and well-being. Currently available assays measure 25-hydroxy vitamin D (25-OHD), a major circulating form of vitamin D. Available methodologies include immunoassays and mass spectrometry based methods (LC-MS/MS). Until recently, the only immunoassays available for diagnostic use in the US have been DiaSorin radioimmunoassay (RIA) and an automated immunoassay on a LIAISON ® platform. Within the last year, Siemens and Abbott successfully launched immunoassays for determination of total vitamin D on their respective automated platforms, Centaur ® and ARCHITECT ® . Development of robust and precise Vitamin D immunoassays has historically been plagued with difficulty. One of the major challenges is development of specific antibodies against such a small antigen. Vitamin D is also highly hydrophobic molecule predominantly bound to vitamin D binding protein (DBP). It is likely, therefore, that immunoassays might be affected to varying extent by the DBP concentration. Adoption of LC-MS/MS into clinical laboratories has enabled development of accurate and almost fully automated methods that could handle increasing volume demands, especially in large volume reference laboratories. Smaller to mid-size hospital laboratories as well as physician offices have neither funds nor technical expertise to implement LC-MS/MS based testing. Our laboratory at the University of Chicago Medical Center has also seen increase in vitamin D volume and currently performs close to 20,000 25-OHD assays per year. We have recently developed an LC-MS/MS method for quantitation of 25-OHD 2 (obtained from plant sources) and 25-OHD 3 (endogenous and animal sources). Prior to acquisition of LC-MS/MS Kratak sadr`aj: Broj zahteva za odre|ivanjem vitamina D je u konstantnom porastu {irom sveta, delom zbog sve vi{e dokaza koji povezuju status vitamina D sa op{tim zdravljem. Trenutno raspolo`ivim testovima odre|uje se 25-hidroksi vitamin D (25-OHD), glavni oblik vitamina D u cirkulaciji. Po stoje}e metodologije uklju~uju imunometrijska odre |i vanja i tehnike zasnovane na masenoj spektrometriji (LC-MS/MS). Do nedavno, jedine raspolo`ive imunometrijske metode kori{}ene za dijagnostiku u SAD su bile DiaSorin radioimunoodre|ivanje (RIA) i automatizovano imuno odre|ivanje na LIAISON ® platformi. U toku pro{le godine Siemens i Abbott su uspe{no lansirali imunometrijske testove za odre|ivanje ukupnog vitamina D na svojim odgova raju}im automatizovanim platformama, Centaur ® i ARCHI-TECT ® . Razvoj robusnih i preciznih imunometrijskih testova za odre|ivanje vitamina D su, istorijski gledano, pratili problemi. Jedan od najve}ih izazova je razvoj specifi~nih antitela protiv malog antigena. Vitamin D je tako|e jako hidrofoban molekul predominantno vezan za vitamin D vezu ju}i protein (DBP). Stoga postoji verovatno}a da na imuno odre|ivanja u razli~itom stepenu mo`e da uti~e koncentracija DBP. Uvo|enje LC-MS/MS u...

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Determining Vitamin D Status: A Comparison between Commercially Available Assays

Cited by 195 publications

References 33 publications

Aging human body: changes in bone, muscle and body fat with consequent changes in nutrient intake

Aging human body: changes in bone, muscle and body fat with consequent changes in nutrient intake

Vitamin D supplementation does not improve human skeletal muscle contractile properties in insufficient young males

Analytical methods and performance of the immunoassay methods for determination of vitamin d in comparison to mass spectrometry / Analitičke metode i izvođenje imunometrijskih određivanja vitamina d u poređenju sa masenom spektrometrijom

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