Detoxification and reproductive system-related gene expression following exposure to Cu(OH)2 nanopesticide in water flea (Daphnia magna Straus 1820)

Aksakal, Feyza İçoğlu; Arslan, Harun

doi:10.1007/s11356-019-07414-x

Cited by 33 publications

(15 citation statements)

References 29 publications

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“…Firstly, it was calculated that the 96 hpf LC 50 value of CNPE was 6.258 mg/l for zebrafish embryos. In literature, the LC 50 value of CNPE was only reported for Daphnia magna , and the value was 2.40 mg/l 19 . On the other hand, LC 50 values of nanopesticides and nanoparticles in fish were limitedly given.…”

Section: Discussionmentioning

confidence: 99%

“…Cu(OH) 2 nanopesticide (CNPE) (Kocide 3000) was purchased from Dupond (Midland, Michigan, USA). Detailed physicochemical properties (ie, the primary particle size, hydrodynamic diameter, and zeta potential) of Kocide 3000 have already been given by Aksakal, Arslan 19 The stock solution of CNPE (100 mg/l) was prepared with Hank's solution and stored at 5°C until the experiments. RNA isolation and SYBR Green RT‐PCR kits were obtained from Qiagen (Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

“…21 The exposure concentrations of CNPE were 1.0, 2.0, 4.0, 8.0, and 16.0 mg/l, and the exposure time was 96 hpf. The selection of exposure concentration was based on Aksakal, Arslan 19 Aksakal, Ciltas, 22 and our preliminary tests. Thirtyfour hpf embryos were randomly selected for each experimental group and control.…”

Section: Developmental Toxicity Testmentioning

confidence: 99%

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Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Aksakal

Şi̇şman

2020

Environmental Toxicology

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The current study evaluates the adverse effects of Cu(OH)2 nanopesticide (CNPE) on the early life stages of zebrafish (Danio rerio). The developmental toxicity was determined using different parameters such as mortality (including LC50), hatching, heart rates, malformations, and alteration of the gene expressions. Zebrafish embryos (4 hpf‐hours postfertilization) were exposed to 1.0, 2.0, 4.0, 8.0, and 16.0 mg/l CNPE doses until 96 hpf. The 96 hours LC50 was recorded at 6.258 mg/l. Seventy‐two hpf total malformation index values for 2.0, 4.0, and 8.0 mg/l CNPE doses were 4.3, 7.2 and 7.9, respectively. 1.0 mg/l CNPE is not toxic for the zebrafish embryos/larvae. 2.0 to 8.0 CNPE doses caused some abnormalities in embryos/larvae morphology, including lack of body parts, tail deformities, chorda deformity, bubbled head, scoliosis, lordosis, weak or non‐pigmentation, decreased heart rate and larva length. 16.0 mg/l CNPE caused mortality in 72 hpf. The expression levels of seven immune system‐related genes (il‐1β, il‐8, cebp, tlr4, hsp70, NF‐kB, and mtf‐1) were examined. The transcription level of il‐1β, il‐8, tlr4, hsp70, and NF‐kB genes significantly increased in the CNPE exposure groups. While the expression of the mtf‐1 gene considerably decreased, the cebp gene expression level did not change in the 4.0 and 8.0 mg/l CNPE doses. In conclusion, CNPE could induce developmental toxicity with malformations in embryos/larvae and alter the gene expression.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Aksakal

Şi̇şman

2020

Environmental Toxicology

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“…Research into certain applications of nanotechnology in agriculture has become increasingly popular over the last decade, with a particular interest in nanopesticides [ 5 , 6 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. Engineering of novel plant protection products has been gaining more attention than other applications, such as nanosensors or fertilizers.…”

Section: Introductionmentioning

confidence: 99%

Hazard Assessment of the Effects of Acute and Chronic Exposure to Permethrin, Copper Hydroxide, Acephate, and Validamycin Nanopesticides on the Physiology of Drosophila: Novel Insights into the Cellular Internalization and Biological Effects

Demir

Kansız

Doğan

et al. 2022

IJMS

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New insights into the interactions between nanopesticides and edible plants are required in order to elucidate their impacts on human health and agriculture. Nanopesticides include formulations consisting of organic/inorganic nanoparticles. Drosophila melanogaster has become a powerful model in genetic research thanks to its genetic similarity to mammals. This project mainly aimed to generate new evidence for the toxic/genotoxic properties of different nanopesticides (a nanoemulsion (permethrin nanopesticides, 20 ± 5 nm), an inorganic nanoparticle as an active ingredient (copper(II) hydroxide [Cu(OH)2] nanopesticides, 15 ± 6 nm), a polymer-based nanopesticide (acephate nanopesticides, 55 ± 25 nm), and an inorganic nanoparticle associated with an organic active ingredient (validamycin nanopesticides, 1177 ± 220 nm)) and their microparticulate forms (i.e., permethrin, copper(II) sulfate pentahydrate (CuSO4·5H2O), acephate, and validamycin) widely used against agricultural pests, while also showing the merits of using Drosophila—a non-target in vivo eukaryotic model organism—in nanogenotoxicology studies. Significant biological effects were noted at the highest doses of permethrin (0.06 and 0.1 mM), permethrin nanopesticides (1 and 2.5 mM), CuSO4·5H2O (1 and 5 mM), acephate and acephate nanopesticides (1 and 5 mM, respectively), and validamycin and validamycin nanopesticides (1 and 2.5 mM, respectively). The results demonstrating the toxic/genotoxic potential of these nanopesticides through their impact on cellular internalization and gene expression represent significant contributions to future nanogenotoxicology studies.

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“…Recently, there have been several studies investigating the impacts of Cu(OH) 2 nanopesticide on crop plants related to genetic, metabolic, and physiological responses. − There have also been studies on microbial community and soil biodiversity in terms of nitrogen cycling, enzymatic activities, and community structure. − Additionally, there is also evidence that the Cu(OH) 2 nanopesticide adversely impacts aquatic organisms, including respiration of estuarine organisms, fish embryonic development and hatch, transcripts related to the fish immune system, and reduced ability for detoxification and reproduction inDaphnia magna. − However, additional toxicity mechanisms may also occur in aquatic organisms following exposure. Carley et al report that, compared with soil communities, aquatic communities may show increased sensitivity to this nanopesticide.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Interrogation of Metabolic and Bioenergetic Responses of Early-Staged Zebrafish (Danio rerio) to a Commercial Copper Hydroxide Nanopesticide

Wang

Qin

et al. 2021

Environ. Sci. Technol.

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The use of copper hydroxide nanopesticide can pose exposure risks to aquatic organisms. In this study, the toxicity of a copper hydroxide nanopesticide, compared to conventional copper sulfate at environmentally relevant doses, was evaluated using metabolomics and bioenergetic assays in embryonic zebrafish. At a copper concentration of 100 μg/L, the nanopesticide caused higher mortality and deformity compared to copper ions alone; despite higher copper accumulation, increased metallothionein and elevated ATP-binding cassette (ABC) transporter activity in zebrafish exposed to copper ions were observed. Both nanopesticide and copper ions reduced the abundance of metabolites of glycolysis and induced energetic stress in zebrafish. The nanopesticide also increased concentrations of several organic acids involved in the tricarboxylic acid (TCA) cycle and elevated the activity of isocitrate dehydrogenase and α-ketoglutarate dehydrogenase, suggesting enhanced TCA cycle activity. Nanopesticide exposure depleted both glutamate and glutamine parallel to the upregulation of the TCA cycle. In addition, zebrafish exposed to the nanopesticide appeared to shift metabolism toward amino acid catabolism and lipid accumulation based upon altered expression profiles of glutaminase, glutamate dehydrogenase, fatty acid synthase, and acetyl-CoA carboxylase. Lastly, the ability of the ions to increase oxidative phosphorylation to alleviate energetic stress was reduced in the case of the nanopesticide. We hypothesize that, unlike copper ions alone, the nanopesticide induces higher toxicity to zebrafish because of increased protein catabolism. This study provides a comprehensive understanding of the risks of copper hydroxide nanopesticide exposure in relation to metabolic activity and mitochondrial function.

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Detoxification and reproductive system-related gene expression following exposure to Cu(OH)2 nanopesticide in water flea (Daphnia magna Straus 1820)

Cited by 33 publications

References 29 publications

Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Hazard Assessment of the Effects of Acute and Chronic Exposure to Permethrin, Copper Hydroxide, Acephate, and Validamycin Nanopesticides on the Physiology of Drosophila: Novel Insights into the Cellular Internalization and Biological Effects

Comprehensive Interrogation of Metabolic and Bioenergetic Responses of Early-Staged Zebrafish (Danio rerio) to a Commercial Copper Hydroxide Nanopesticide

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Detoxification and reproductive system-related gene expression following exposure to Cu(OH)2 nanopesticide in water flea (Daphnia magna Straus 1820)

Cited by 33 publications

References 29 publications

Developmental toxicity induced by Cu(OH)2 nanopesticide in zebrafish embryos

Developmental toxicity induced by Cu(OH)2 nanopesticide in zebrafish embryos

Hazard Assessment of the Effects of Acute and Chronic Exposure to Permethrin, Copper Hydroxide, Acephate, and Validamycin Nanopesticides on the Physiology of Drosophila: Novel Insights into the Cellular Internalization and Biological Effects

Comprehensive Interrogation of Metabolic and Bioenergetic Responses of Early-Staged Zebrafish (Danio rerio) to a Commercial Copper Hydroxide Nanopesticide

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Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos

Developmental toxicity induced by Cu(OH)₂ nanopesticide in zebrafish embryos