Detrimental effect of hydrosalpinx fluid on the development and blastulation of mouse embryos in vitro

Sachdev, Rahul; Kemmann, Ekkehard; Bohrer, Michael; El-Danasouri, Iman

doi:10.1016/s0015-0282(97)00238-0

Cited by 38 publications

(13 citation statements)

References 14 publications

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“…One metaanalysis has clearly demonstrated a pregnancy probability reduction and an increased spontaneous abortion risk in the presence of hydrosalpinx (1). Experimental results were in line with this clinical observations (13)(14)(15)(16). Moreover, salpingectomy has shown its positive impact on endometrial receptivity (17) and IVF results.…”

supporting

confidence: 87%

Management of hydrosalpinx before or during in vitro fertilization–embryo transfer: a national postal survey in France

Ducarme

Uzan

Hugues

et al. 2006

Fertility and Sterility

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supporting

confidence: 87%

Management of hydrosalpinx before or during in vitro fertilization–embryo transfer: a national postal survey in France

Ducarme

Uzan

Hugues

et al. 2006

Fertility and Sterility

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“…Several investigators did demonstrate HSF induced embryotoxicity even at very low concentrations (i.e. less than 10%) (9)(10)(11)(12). Others noted an embryo toxic effect only at high concentrations (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…The tubal epithelium may secrete cytokines, leukotrienes or prostaglandins into the sequestered fluid that could directly alter endometrial function (8). It has been also speculated that the negative influence of HSF may be due to toxic substances in the fluid (9)(10)(11)(12)(13)(14). Others have reported no adverse effect of hydrosalpinges on IVF-ET (15).…”

Section: Introductionmentioning

confidence: 99%

Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid

Falcone

Goldberg

Attaran

et al. 2005

J Assist Reprod Genet

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Objective : The exact chemical composition of hydrosalpingeal fluid is unknown. The objective of this study was to characterize cytokines in hydrosalpingeal fluid (HSF) and examine their possible role in the embryo development. Study Design : HSF was aspirated at laparoscopic salpingectomy in eight infertile women. Levels of IL-1β, IL-13, IL-8, IL-6 and TNF-α in the HSF were determined by quantitative immunoassay kits. Two-cell mouse embryos were incubated with 0, 25, 50 and 75% concentrations of HSF. The blastocyst development rate (BDR) of mouse embryos was measured at each HSF concentration. Result(s) :The embryotoxicity of HSF was concentration dependent. An increase in the HSF concentration resulted in significant decrease in % BDR (p < 0.01). IL-1β was present in six of the eight HSF samples with a mean (±SD) concentration of 0.9 ± 0.8 pg/mL. IL-13 was not detected in any of the HSF samples. IL-8, IL-6 and TNF-α were detected in all samples with a mean (±SD) concentration of 4741.2 ± 6554.4 pg/mL, 204.8±132.8 pg/ml and 12 ± 12.8 pg/mL respectively. IL-6 was positively correlated with BDR (r = 0.53; p < 0.04). Conclusion(s) :We demonstrated for the first time the presence of IL-1β, IL-8, IL-6 and TNF-α and the absence of IL-13 in human hydrosalpingeal fluid. IL-6 was positively related to the BDR.

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“…The presence of HSP during in vitro fertilization and embryo transfer (IVF/ET) is associated with decreased implantation, pregnancy and increased rate of early pregnancy loss (Aboulghar et al, 1998;Zeyneloglu et al, 1998;Camus et al, 1999). Although toxic effects of hydrosalpinx fluid (HF) on mouse embryo development (Mukherjee et al, 1996;Beyler et al, 1997;Murray et al, 1997;Rawe et al, 1997;Sachdev et al, 1997;Koong et al, 1998;Spandorfer et al, 1999;Roberts et al, 1999;Carrasco et al, 2001;de Vantery Arrighi et al, 2001) and human spermatozoa in vitro (Ng et al, 2000) have been documented, the mechanisms underlying the adverse effects are far from understood (Ajonuma et al, 2002). Puttemans and Brosens (1996) and Vasquez et al (1995) proposed three grades of HSP based on the appearance of salpingoscopy.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural characterization of whole hydrosalpinx from infertile Chinese women

Ajonuma

Chan

et al. 2005

Cell Biology International

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Hydrosalpinx (HSP) has been shown to be detrimental to the outcome of assisted reproduction, but little is known of its pathology. This prospective study examined and detailed ultrastructural characterization of HSP of infertile women presenting for assisted reproductive treatments. Both light and electron microscopies were used to characterize HSP. Hematoxylin and eosin staining of HSP showed areas without epithelial cell lining or with abnormalities such as flattening of the epithelial layer and exfoliation of epithelial cells with occasional normal columnar epithelial lining. HSP muscle fibers were atrophic and occasionally replaced by fibrous tissues, or separated by areas of severe edema. Inflammatory cells could be found in hydrosalpinx fluid (HF) in the lumen in areas with flattened to no epithelial cells, without epithelial lining, as well as in dilated blood vessels and/or lymph vessels. Scanning electron microscopy of the epithelial surface revealed epithelial denudation-severe loss of both cilia and microvilli and stomata exuding globular bodies on eroded ampulla surfaces. Severe chronic inflammation and damage to the epithelial lining and musculature of Fallopian tubes and the presence of inflammatory cells provides an explanation for HF formation, and thus for the detrimental effects of HF on reproductive processes and IVF outcome.

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Detrimental effect of hydrosalpinx fluid on the development and blastulation of mouse embryos in vitro

Cited by 38 publications

References 14 publications

Management of hydrosalpinx before or during in vitro fertilization–embryo transfer: a national postal survey in France

Management of hydrosalpinx before or during in vitro fertilization–embryo transfer: a national postal survey in France

Relationship between cytokines and the embryotoxicity of hydrosalpingeal fluid

Ultrastructural characterization of whole hydrosalpinx from infertile Chinese women

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