Anxiety is a common emotion of driver, which always affects the safety of driving. Eye movement characteristics can be used to understand the true emotion state of human beings. It is of great significance to study the law of eye movement for realizing active vehicle safety warning and human-machine cooperation. In this article, anxiety-induction experiment, real-vehicle driving experiments, and virtual driving experiments were designed and used to obtain the eye movement data of female novice extroversion driver under calm and anxiety, and mathematical statistics analysis was made on the fixation count, fixation duration, and visit duration in the area of interest within the driver horizon. The results showed that there are significant differences in fixation count and fixation duration of drivers (p\0:05, p is the accompanying probability), and the main effect of emotion is significant (p\0:05).Compared with the situation of calm, fixation area, fixation count, and fixation duration of drivers under anxiety were more focused on the middle area, the fixation count and visit duration on the left area were relatively more, the fixation duration on the right area was relatively longer, and anxiety was more likely to cause driver's attention bias.