Deuterated stearic acid uptake and accumulation in lipid droplets of cat oocytes

Ranneva, S.V.; Okotrub, Konstantin A.; Amstislavsky, S. Ya.; Surovtsev, N. V.

doi:10.1016/j.abb.2020.108532

Cited by 19 publications

(5 citation statements)

References 42 publications

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“…As cells grow in a deuterated FA-supplemented culture medium, they incorporate deuterated FAs in membranes and lipid droplets. It has been verified that the alternation from nondeuterated FAs to deuterated FAs does not affect cellular activity; hence, a deuterated FA is an ideal probe for studying cellular FA uptake . Previous research has shown that combined with deuterium labeling, SRS microscopy can be used to explore and quantify FA uptake in single cells and tissues. − To achieve accurate quantification of FA metabolism, it is important to image the sample at both the C–D and C–H regions concurrently to minimize errors caused by laser fluctuation, power variation, or focus drifting.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Dual-Band Hyperspectral Stimulated Raman Scattering Microscopy with Femtosecond Optical Parametric Oscillators

Rathbone

2023

J. Phys. Chem. B

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Stimulated Raman scattering (SRS) microscopy is a label-free quantitative optical technique for imaging molecular distributions in cells and tissues by probing their intrinsic vibrational frequencies. Despite its usefulness, existing SRS imaging techniques have limited spectral coverage due to either a wavelength tuning constraint or narrow spectral bandwidth. High-wavenumber SRS imaging is commonly used to map lipid and protein distribution in biological cells and visualize cell morphology. However, to detect small molecules or Raman tags, imaging in the fingerprint region or “silent” region, respectively, is often required. For many applications, it is desirable to collect SRS images in two Raman spectral regions simultaneously for visualizing the distribution of specific molecules in cellular compartments or providing accurate ratiometric analysis. In this work, we present an SRS microscopy system using three beams generated by a femtosecond oscillator to acquire hyperspectral SRS image stacks in two arbitrary vibrational frequency bands, between 650–3280 cm–1, simultaneously. We demonstrate potential biomedical applications of the system in investigating fatty acid metabolism, cellular drug uptake and accumulation, and lipid unsaturation level in tissues. We also show that the dual-band hyperspectral SRS imaging system can be adapted for the broadband fingerprint region hyperspectral imaging (1100–1800 cm–1) by simply adding a modulator.

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Section: Resultsmentioning

confidence: 99%

Simultaneous Dual-Band Hyperspectral Stimulated Raman Scattering Microscopy with Femtosecond Optical Parametric Oscillators

Rathbone

2023

J. Phys. Chem. B

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“…Deuterium is also commonly used as a Raman tag to visualize lipids (112,113). Advances in the understanding of lipid physiology created through the use of deuterium tags include the visualization of lipid translocation between endocytic vesicles and LDs in macrophages (114), intracellular lipid metabolism in macrophages (113), time-dependent investigation of FA distribution in macrophages (115), and the quantification of stearic acid uptake and accumulation in LDs of cat oocytes (116). One recent innovation incorporated H-alkyne and D-alkyne labels into long-chain FA probes to distinguish between two structurally similar small molecules in living cells (117).…”

Section: Spontaneous Raman Imagingmentioning

confidence: 99%

Analytical Techniques for Single-Cell Biochemical Assays of Lipids

Yao

Vaithiyanathan

Allbritton

2023

Annu. Rev. Biomed. Eng.

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Lipids are essential cellular components forming membranes, serving as energy reserves, and acting as chemical messengers. Dysfunction in lipid metabolism and signaling is associated with a wide range of diseases including cancer and autoimmunity. Heterogeneity in cell behavior including lipid signaling is increasingly recognized as a driver of disease and drug resistance. This diversity in cellular responses as well as the roles of lipids in health and disease drive the need to quantify lipids within single cells. Single-cell lipid assays are challenging due to the small size of cells (∼1 pL) and the large numbers of lipid species present at concentrations spanning orders of magnitude. A growing number of methodologies enable assay of large numbers of lipid analytes, perform high-resolution spatial measurements, or permit highly sensitive lipid assays in single cells. Covered in this review are mass spectrometry, Raman imaging, and fluorescence-based assays including microscopy and microseparations. Expected final online publication date for the Annual Review of Biomedical Engineering, Volume 25 is June 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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“…Interestingly, higher concentration of saturated fatty acids even affects the post-fertilization developmental competence of in vitro matured oocytes, while monounsaturated fatty acids like oleic acid initiate the normal developmental competence. The adverse effects of saturated fatty acids can be reversed by the addition of a balanced amount of palmitic acid (C16) (Shibahara et al, 2020), stearic acid (C18) (Ranneva et al, 2020), and oleic acid during in vitro maturation of oocytes (Wu et al, 2010;Aardema et al, 2011). Thus, the composition as well as a balanced amount of saturated and unsaturated free fatty acids in the FF is critical for the developmental competence of oocytes.…”

Section: Cumulus-oocyte Complex: the Dynamic Unit Of Oocyte Metabolismmentioning

confidence: 99%

Role of Lipid Metabolism and Signaling in Mammalian Oocyte Maturation, Quality, and Acquisition of Competence

Khan

Jiang

Hameed

et al. 2021

Front. Cell Dev. Biol.

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It has been found that the quality of oocytes from obese women has been compromised and subsequent embryos displayed arrested development. The compromised quality may be either due to the poor or rich metabolic conditions such as imbalance or excession of lipids during oocyte development. Generally, lipids are mainly stored in the form of lipid droplets and are an important source of energy metabolism. Similarly, lipids are also essential signaling molecules involved in various biological cascades of oocyte maturation, growth and oocyte competence acquisition. To understand the role of lipids in controlling the oocyte development, we have comprehensively and concisely reviewed the literature and described the role of lipid metabolism in oocyte quality and maturation. Moreover, we have also presented a simplified model of fatty acid metabolism along with its implication on determining the oocyte quality and cryopreservation for fertilization.

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Deuterated stearic acid uptake and accumulation in lipid droplets of cat oocytes

Cited by 19 publications

References 42 publications

Simultaneous Dual-Band Hyperspectral Stimulated Raman Scattering Microscopy with Femtosecond Optical Parametric Oscillators

Simultaneous Dual-Band Hyperspectral Stimulated Raman Scattering Microscopy with Femtosecond Optical Parametric Oscillators

Analytical Techniques for Single-Cell Biochemical Assays of Lipids

Role of Lipid Metabolism and Signaling in Mammalian Oocyte Maturation, Quality, and Acquisition of Competence

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